@article{mbs:/content/journal/jgv/10.1099/0022-1317-67-4-719, author = "McMichael, Andrew J. and Michie, Colin A. and Gotch, Frances M. and Smith, Geoffrey L. and Moss, Bernard", title = "Recognition of Influenza A Virus Nucleoprotein by Human Cytotoxic T Lymphocytes", journal= "Journal of General Virology", year = "1986", volume = "67", number = "4", pages = "719-726", doi = "https://doi.org/10.1099/0022-1317-67-4-719", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-4-719", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "CTL", keywords = "NP", keywords = "influenza A virus", abstract = "Summary A recombinant vaccinia virus (NP-VAC) containing cDNA corresponding to segment 5, the nucleoprotein (NP) gene of influenza A/PR/8/34 virus was used to examine the specificity of human influenza virus immune cytotoxic T lymphocytes (CTL). Effector cell preparations from two donors recognized autologous lymphocytes that had been infected with NP-VAC. Lysis was specific because cells infected with vaccinia virus were not killed and recognition was HLA-restricted. In one donor, the influenza virus-specific CTL response changed with time so that his effector cells no longer recognized autologous lymphocytes infected with NP-VAC. However, a component that was NP-specific remained because these CTL lysed the more sensitive autologous B lymphoblastoid cells that had been infected with NP-VAC. In four other donors, no NP-specific CTL response could be detected using autologous lymphocyte targets. Thus NP, an internal virus protein, is one antigen that is recognized by human influenza A virus-specific CTL, but it is likely that other individual virus components contribute to the total CTL response.", }