Three clones of Daudi cells, DIF, DIF and DIF, have been isolated which possess specific interferon (IFN) receptors even though these cells are resistant to both the antiviral and antiproliferative actions of human IFN-α. Studies with I-labelled cloned human IFN-α subspecies showed that clone DIF bound IFN in a manner identical to that of the IFN-sensitive parental cells. The initial peak of binding observed 30 min after treatment of either Daudi or DIF cells at 37 °C with IFN-α or IFN-α was, however, either absent or much reduced when using DIF cells which are resistant to the antiproliferative action of IFN-α and -α. Similarly, no initial peak of binding was observed after treatment of DIF cells with IFN-α at 37 °C. In accord with its reduced biological activity, the binding of I-IFN-α to either Daudi or DIF cells was considerably lower than that of either of the other two IFN-α subspecies and followed the form of a hyperbola without an initial transitory peak of binding. It is suggested that the phenotype of IFN resistance of DIF and DIF cells may be related to a defect in receptor activation whereas the same phenotype of DIF cells would appear to be unrelated to IFN binding.

Keyword(s): binding , IFN (HuIFN-α) and receptors

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