Murine Cytotoxic T Cells Specific to Respiratory Syncytial Virus Recognize Different Antigenic Subtypes of the Virus Free

Abstract

Summary

The aim of this study was to establish whether cytotoxic T cells (Tc), raised against respiratory syncytial virus (RSV) in the mouse, are specific to the strain of immunizing virus, or cross-reactive between virus strains. Several recent studies using monoclonal antibodies have begun to define the antigenic variation among strains of RSV. It is likely that a successful RSV vaccine will need to contain antigenic determinants from more than one subtype, but since only the highest levels of neutralizing antibody are able to give complete protection against RSV infection, a vaccine may also need to elicit a cellular immune response. We have recently described H-2-restricted, RSV-specific Tc following RSV infection in the mouse; we present here evidence that polyclonal RSV-specific Tc in the mouse recognize syngeneic target cells infected with every human strain of RSV tested, whatever the subtype. The only RSV strain that appeared not to be recognized was bovine RSV, which seems unable to infect mouse cells; however, bovine cells, infected with bovine RSV and fixed with glutaraldehyde, primed mice for Tc which recognized human strains of RSV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-4-623
1986-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/4/JV0670040623.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-4-623&mimeType=html&fmt=ahah

References

  1. Anderson L. J., Hierholzer I. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., McIntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Bangham C. R. M., Cannon M. J., Karzon D. T., Askonas B. A. 1985; Cytotoxic T-cell response to respiratory syncytial virus in mice. Journal of Virology 56:55–59
    [Google Scholar]
  3. Beem M. 1967; Repeated infections with respiratory syncytial virus. Journal of Immunology 98:1115–1122
    [Google Scholar]
  4. Coates H. V., Chanock R. M. 1962; Experimental infection with respiratory syncytial virus in several species of animals. American Journal of Hygiene 76:302–312
    [Google Scholar]
  5. Coates H. V., Kendrick L., Chanock R. M. 1963; Antigenic differences between two strains of respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 112:958–964
    [Google Scholar]
  6. Coates H. V., Along D. W., Chanock R. M. 1966; An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. American Journal of Epidemiology 83:299–312
    [Google Scholar]
  7. Doggett J. E., Taylor-Robinson D. 1965; Serological studies with respiratory syncytial virus. Archiv für die gesämte Virusforschung 15:601–608
    [Google Scholar]
  8. Downham M. A. P. S., Scott R., Sims D. G., Webb J. K. G., Gardner P. S. 1976; Breast-feeding protects against respiratory syncytial virus infections. British Medical Journal ii:274–276
    [Google Scholar]
  9. Fernie B. F., Ford E. C., Gerin J. L. 1981; The development of BALB/c cells persistently infected with respiratory syncytial virus: presence of ribonucleoprotein on the cell surface. Proceedings of the Society for Experimental Biology and Medicine 167:83–86
    [Google Scholar]
  10. Fishaut M., Tubergen D., McIntosh K. 1979; Prolonged fatal respiratory viral infections in children with disorders of cell-mediated immunity. Pediatric Research 13:447
    [Google Scholar]
  11. Gimenez H. B., Cash P., Melvin W. T. 1984; Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Journal of General Virology 65:963–971
    [Google Scholar]
  12. Glezen W. P., Paredes A., Allison J. E., Taber L. H., Frank A. L. 1981; Risk of respiratory syncytial virus for infants from low-income families in relationship to age, sex, ethnic group, and maternal antibody level. Journal of Pediatrics 98:705–715
    [Google Scholar]
  13. Henderson F. W., Collier A. M., Clyde W. A., Denny F. W. 1979; Respiratory-syncytial-virus infections, reinfections and immunity. New England Journal of Medicine 300:530–534
    [Google Scholar]
  14. Karzon D. T. 1983; The immune basis for hypersensitivity to viral vaccines. In Human Immunity to Viruses pp. 111–130 Edited by Ennis F. A. New York: Academic Press;
    [Google Scholar]
  15. McIntosh K., Fishaut J. M. 1980; Immunopathologic mechanisms in lower respiratory tract disease of infants due to respiratory syncytial virus. Progress in Medical Virology 26:94–118
    [Google Scholar]
  16. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  17. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington, D. C. II. Infection and disease with respect to age, immunologic status, race and sex. American Journal of Epidemiology 98:289–300
    [Google Scholar]
  18. Prince G. A., Horswood R. L., Chanock R. M. 1985; Quantitative aspects of passive immunity to respiratory syncytial virus in infant cotton rats. Journal of Virology 55:517–520
    [Google Scholar]
  19. Stott E. J., Bew M. H., Taylor G., Jebbett J., Collins A. P. 1984a; The characterisation and uses of monoclonal antibodies to respiratory syncytial virus. Developments in Biological Standardization 57:237–244
    [Google Scholar]
  20. Stott E. J., Thomas L. H., Taylor G., Collins A. P., Jebbett J., Crouch S. 1984b; A comparison of three vaccines against respiratory syncytial virus in calves. Journal of Hygiene 93:251–261
    [Google Scholar]
  21. Sun C. -S., Wyde P. R., Wilson S. Z., Knight V. 1983; Cell-mediated cytotoxic responses in lungs of cotton rats infected with respiratory syncytial virus. American Review of Respiratory Diseases 127:460–464
    [Google Scholar]
  22. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  23. Townsend A. R. M., Skehel J. J. 1984; The influenza A virus nucleoprotein gene controls the induction of both subtype specific and cross-reactive cytotoxic T cells. Journal of Experimental Medicine 160:552–563
    [Google Scholar]
  24. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
  25. Ward K. A., Everson J. S., Lambden P. R., Watt P. J. 1984; Antigenic and structural variation in the major nucleocapsid protein of respiratory syncytial virus. Journal of General Virology 65:1749–1757
    [Google Scholar]
  26. Zweerink H. J., Askonas B. A., Millican D., Courtneidge S. A., Skehel J. J. 1977; Cytotoxic T cells to type A influenza virus: viral haemagglutinin induces A-strain specificity while infected cells confer cross-reactive cytotoxicity. European Journal of Immunology 7630–635
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-4-623
Loading
/content/journal/jgv/10.1099/0022-1317-67-4-623
Loading

Data & Media loading...

Most cited Most Cited RSS feed