In particles of turnip yellow mosaic virus (TYMV), the interactions between proteins are particularly strong when compared to those between proteins in some other icosahedral viruses. Intact RNA is released from TYMV particles on freezing and this process has been studied by examining several parameters that influence cryodenaturation such as dehydration, pressure, aggregation and the presence of protective agents (glycerol and ammonium sulphate). Pressure of 1.5 × 10 Pa had no effect on virus particles whereas dehydration of a virus suspension had a drastic effect. Aggregate formation resulting from freezing of solutions containing high virus concentrations seems to be a prerequisite for RNA release; cryoprotective agents hindered RNA release.


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