1887

Abstract

Summary

Five rat monoclonal antibodies (MAbs) specific for U.K. isolate B of barley yellow dwarf virus (BYDV) have been produced and tested. The reactions of the MAbs against a range of isolates of known vector specificity from the U.K., U.S.A. and Sweden were compared and a provisional serological correlation suggested. A survey of 90 U.K. field isolates showed that they were of the same serological groupings as the typed isolates, and that the panel of MAbs could be used to characterize field isolates of BYDV. Two of the MAbs were produced in large quantities in ascitic fluids; antibody partially purified from them and conjugated to alkaline phosphatase was used successfully in direct ELISA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-3-549
1986-03-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-3-549
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error