Virus particles were isolated from hypertrophied salivary glands of the tsetse fly, Glossina pallidipes collected near Mombasa, Kenya. Purified virus particles were rodshaped, 57 nm wide by 700 to 1300 nm long. Particle lengths fell into two size classes, with ‘short’ particles averaging 869 nm and ‘long’ particles 1175 nm. The virus particles morphologically resembled elongated baculovirus nucleocapsids although, unlike baculoviruses, no fully enveloped virions were found in purified preparations. The particles contained double-stranded DNA which appeared to be linear when analysed by electrophoresis in agarose gels, ethidium bromide-caesium chloride gradient centrifugation or electron microscopy (EM). There was some evidence for the DNA being heterogeneous in size from EM studies and from the observation that restriction enzyme analysis failed to provide a clear profile of DNA fragments. Protein from purified virions contained at least 12 polypeptides with a major component of 39000 mol. wt. These results suggest that the virus cannot be placed in any of the existing taxonomic groupings of DNA viruses.
AmargierA.,
LyonJ.-P.,
VagoC.,
MeynadierG.,
VeyrunesJ.-C.1979; Mise en evidence et purification d’un virus dans la proliferation monstreuse glandulaire d’insectes. Etude sur Merodon equestris F. (Diptere, Syrphidae). Comptes rendus hebdomadaires des seances de l’Academic des sciences, série D 289:481–484
BudH. M.,
KellyD. C.1977; The DNA contained by nuclear polyhedrosis viruses isolated from four Spodoptera spp. (Lepidoptera, Noctuidae); genome size and configuration assessed by electron microscopy. Journal of General Virology 37:135–143
ChallierA.,
LaveissièreC.1973; Un nouveau piège pour la capture des glossines (Glossina: Diptera, Muscidae): description et essais sur le terrain. Cahiers ORSTOM: Série Entomologie Medicate et Parasitologie 11:251–262
GonzalezJ. M.Jr,
DulmageH. T.,
CarltonB. C.1981; Correlation between specific plasmids and δ- endotoxin production in Bacillus thuringiensis. Plasmid 5:351–365
JaensonT. G. T.1978; Virus-like rods associated with salivary gland hyperplasia in tsetse, Glossina pallidipes. Transactions of the Royal Society of Tropical Medicine and Hygiene 72:234–238
JenniL.1973; Virus–like particles in a strain of G. morsitans centralis, Machado 1970. Transactions of the Royal Society of Tropical Medicine and Hygiene 67:295
LowryO. H.,
RosebroughN. J.,
FarrA. L.,
RandallR. J.1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
OdindoM. O.1982; Incidence of salivary gland hypertrophy in field populations of the tsetse Glossina pallidipes on the South Kenyan coast. Insect Science and its Application 3:59–64
OdindoM. O.,
SabwaD. M.,
AmutallaP. A.,
OtienoW. A.1981; Preliminary tests on the transmission of virus-like particles to the tsetse Glossina pallidipes. Insect Science and its Application 2:219–221
OtienoL. H.,
KokwaroE. D.,
ChimtawiM.,
OnyangoP.1980; Prevalence of enlarged salivary glands in wild populations of Glossina pallidipes in Kenya, with a note on the ultrastructure of the affected organ. Journal of Invertebrate Pathology 36:113–118
ShatkinA. J.1969; Colorimetric reactions for DNA, RNA, and protein determinations. In Fundamental Techniques in Virology pp. 231–237 Edited by
HabelK.,
SalzmanN. P.
New York: Academic Press;
WhitnallA. B. M.1934; The trypanosome infections of Glossina pallidipes in the Umfolosi game reserve, Zululand. Onderstepoort Journal of Veterinary Science and Animal Industry 11:7–21