Regurgitant from leaf-feeding beetles ( and ) contains RNase activity equivalent to 0.1 to 1 mg/ml pancreatic RNase. When mixtures of pancreatic RNase at 0.1 to 1.0 mg/ml and viruses that are or are not transmissible by beetles, were inoculated to plants using the gross wounding technique, those containing viruses not transmissible by beetles were not infective. Pancreatic RNase at activities up to 25 times that found in beetle regurgitant did not prevent transmission of two beetle-transmissible viruses, whereas RNase at activities one-half that found in beetle regurgitant prevented or greatly inhibited transmission of two viruses not transmitted by beetles.

Keyword(s): beetle , RNase and transmission

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