1887

Abstract

Summary

Hybridomas secreting monoclonal antibodies against three isolates of barley yellow dwarf virus (BYDV) were established. Two monoclonal antibody preparations were generated against the MAV isolate, one against RPV and six against P-PAV. None of the monoclonal antibody preparations reacted with healthy host components. Reactions of monoclonal antibodies, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicated that all three virus isolates share a common epitope. BYDV particles dissociated when incubated in carbonate-bicarbonate coating buffer at pH 9.6 but could be stabilized by prior dialysis against 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized and dissociated particles of homologous and heterologous BYDV isolates. However, conjugated polyclonal antisera were incapable of binding to dissociated particles or to stabilized particles of heterologous isolates. Experiments with monoclonal antibodies in a competition ELISA indicated the presence of at least two epitopes on the coat protein of P-PAV.

Keyword(s): BYDV , ELISA and monoclonal antibodies
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-353
1986-02-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020353.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-353&mimeType=html&fmt=ahah

References

  1. Al Moudallal Z., Briand J. P., Van Regenmortel M. H. V. 1982; Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 1:1005–1010
    [Google Scholar]
  2. Bayer E. A., Skutelsky E., Wilchek M. 1979; The avidin-biotin complex in affinity cytochemistry. Methods in Enymology 62:308–326
    [Google Scholar]
  3. Briand J. P., Ae Moudallal Z., Van Regenmortel M. H. V. 1982; Serological differentiation of tobamoviruses by means of monoclonal antibodies. Journal of Virological Methods 5:293–300
    [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 32:475–483
    [Google Scholar]
  5. Diaco R., Lister R. M., Durand D. P., Hill J. H. 1983; Production of monoclonal antibodies against three isolates of barley yellow dwarf virus. Phytopathology 73:788
    [Google Scholar]
  6. Diaco R., Hill J. H., Lister R. M., Durand D. P. 1984; Use of monoclonal antibodies in serologically specific electron microscopy of barley yellow dwarf virus. Phytopathology 74:860
    [Google Scholar]
  7. Diaco R., Lister R. M., Hill J. H., Durand D. P. 1986; Detection of homologous and heterologous barley yellow dwarf virus isolates with monoclonal antibodies in serologically specific electron microscopy. Phytopathology (in press)
    [Google Scholar]
  8. Galfré G., Milstein C. 1981; Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73:3–46
    [Google Scholar]
  9. Gugerli P., Fries P. 1983; Use of monoclonal antibodies to potato virus Y and their use for virus detection. Journal of General Virology 64:2471–2477
    [Google Scholar]
  10. Hammond J., Lister R. M., Foster J. E. 1983; Purification, identity and some properties of an isolate of barley yellow dwarf virus from Indiana. Journal oj General Virology 64:667–676
    [Google Scholar]
  11. Hill E. K., Hill J. H., Durand D. P. 1984; Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. Journal of General Virology 65:525–532
    [Google Scholar]
  12. Hill J. H., Bryant G. R., Durand D. P. 1981; Detection of plant virus by using purified IgG in ELISA. Journal of Virological Methods 3:27–35
    [Google Scholar]
  13. Howard J. C., Butcher G. W., Galfre G., Milstein C. 1978; Monoclonal anti-rat MHC (H-l) alloantibodies. Current Topics in Microbiology and Immunology 81:54–60
    [Google Scholar]
  14. Howard J. C., Butcher G. W., Galfre G., Milstein C.P. 1979; Monoclonal antibodies as tools to analyze the serological and genetic complexities of major transplantation antigens. Immunological Reviews 47:139–174
    [Google Scholar]
  15. Hsu H. T., Aebig J., Rochow W. F., Lawson R. H. 1983; Isolation of hybridomas secreting antibodies reactive to RPV and MAV isolates of barley yellow dwarf and carnation etched ring virus. Phytopathology 73:790
    [Google Scholar]
  16. Hsu H. T., Aebig J., Rochow W. F. 1984; Differences among monoclonal antibodies to barley yellow dwarf viruses. Phytopathology 74:600–605
    [Google Scholar]
  17. Johnson R. A., Rochow W. F. 1972; An isolate of barley yellow dwarf virus transmitted specifically by Schizaphis graminum. Phytopathology 62:921–925
    [Google Scholar]
  18. Koenig R. 1978; ELISA in the study of homologous and heterologous reactions of plant viruses. Journal of General Virology 40:309–318
    [Google Scholar]
  19. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  20. Lister R. M., Rochow W. F. 1979; Detection of barley yellow dwarf virus by enzyme-linked immunosorbent assay. Phytopathology 69:649–654
    [Google Scholar]
  21. Rochow W. F. 1969; Biological properties of four isolates of barley yellow dwarf virus. Phytopathology 59:1580–1589
    [Google Scholar]
  22. Rochow W. F. 1970; Barley yellow dwarf virus. Commonwealth Mycological Institute I Association of Applied Biologists Descriptions of Plant Viruses No 32
    [Google Scholar]
  23. Rochow W. F., Carmichael L. E. 1979; Specificity among barley yellow dwarf viruses in enzyme immunosorbent assays. Virology 95:415–420
    [Google Scholar]
  24. Rochow W. F., Israel H. W. 1977; Luteovirus (barley yellow dwarf virus) group. In The Atlas of Insect and Plant Viruses pp. 363–369 Edited by Maramorosch K. New York: Academic Press;
    [Google Scholar]
  25. Van Deusen R. A., Whetstone C. A. 1981; Practical aspects of producing and using anti-viral monoclonal antibodies as diagnostic reagents. Annual Proceedings of the American Association of Veterinary Laboratory Diagnosticians 24:211–228
    [Google Scholar]
  26. Van Regenmortel M. H. V. 1966; Plant virus serology. Advances in Virus Research 12:207–271
    [Google Scholar]
  27. Voller A., Bidwell D. E., Bartlett A. 1979; The enzyme linked immunosorbent assay (ELISA). Alexandria: Dynatech Laboratories;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-2-353
Loading
/content/journal/jgv/10.1099/0022-1317-67-2-353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error