1887

Abstract

Summary

Immunoaffinity columns were prepared by covalently binding monoclonal antibodies specific for soybean mosaic virus (SMV) to an agarose support matrix. SMV, purified by binding and elution, contained no contaminating proteins detectable by SDS-polyacrylamide gel electrophoresis. Affinity-purified SMV was essentially identical to virus purified by standard procedures in its infectivity, u.v. absorbance spectrum ratio of / , sedimentation coefficient, electrophoretic pattern of coat protein, morphology and antigenicity. This method of purification is rapid, reproducible, and yields highly purified virus preparations. It may therefore be applicable in the purification of a wide variety of plant viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-345
1986-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020345.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-345&mimeType=html&fmt=ahah

References

  1. Akanuma Y., Kuzuya T., Hayashi M., Ide T., Kuzuya N. 1970; Immunological reactivity of insulin to Sepharose coupled with insulin-antibody: its use for extraction of insulin from serum. Biochemical and Biophysical Research Communications 38:947–953
    [Google Scholar]
  2. Bayer E. A., Skutelsky E., Wilchek M. 1979; The avidin-biotin complex in affinity cytochemistry. Methods in Enzymology 62:308–326
    [Google Scholar]
  3. Brakke M. K., Van Pelt N. 1970; Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Analytical Biochemistry 38:56–64
    [Google Scholar]
  4. Delgado-Sanchez S., Grogan R. G. 1966; Purification and properties of potato virus Y. Phytopathology 56:1397–1404
    [Google Scholar]
  5. Erickson R. P., Steers E. 1970; Isozymes of bacterial β-galactosidases: purification and characterization of a nonisoenzyme forming β-galactosidase of Aerobacter cloacae. Archives of Biochemistry and Biophysics 137:399–408
    [Google Scholar]
  6. Hewish D. R., Shukla D. D. 1983; Purification of barley yellow dwarf virus by gel filtration on Sephacryl S-1000 superfine. Journal of Virological Methods 7:223–228
    [Google Scholar]
  7. Hill J. H., Benner H. I. 1980; Properties of soybean mosaic virus and its isolated protein. Phytopathologische Zeitschrift 97:272–281
    [Google Scholar]
  8. Hill E. K., Hill J. H., Durand D. P. 1984; Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. Journal of General Virology 65:525–532
    [Google Scholar]
  9. Jackson B.-E., Jackson A. O., Brakke M. K. 1977; Pipetting calculations for linear-log gradients in Spinco rotors. In Isco Tables 7th edn. pp. 28–29 Edited by Hoffman G. Nebraska: Instrument Specialities Company;
    [Google Scholar]
  10. Kenyon A. J., Gander J. E., Lopez C., Good R. A. 1973; Isolation of Aleutian mink disease virus by affinity chromatography. Science 179:187–189
    [Google Scholar]
  11. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Mclaughlin M. R., Bryant G. R., Hill J. H., Benner H. I., Durand D. P. 1980; Isolation of specific antibody to plant viruses by acid sucrose density gradient centrifugation. Phytopathology 73:831–834
    [Google Scholar]
  14. Matthews R. E. F. 1981; Isolation. In Plant Virology 2nd edn pp 50–68 New York: Academic Press;
    [Google Scholar]
  15. Melchers F., Messer W. 1970; The activation of mutant β-galactosidase by specific antibodies. European Journal of Biochemistry 17:267–272
    [Google Scholar]
  16. Milbrath G. M., Soong M.-M. 1976; A local lesion assay for soybean mosaic virus using Phaseolus vulgaris L. cv. Top Crop. Phytopathologische Zeitschrift 87:255–259
    [Google Scholar]
  17. Murphy R. F., Buchanan K. D., Elmore D. T. 1973; Isolation of glucagon-like immunoreactivity of gut by affinity chromatography on anti-glucagon antibodies coupled to Sepharose 4B. Biochimica et biophysica acta 303:118–127
    [Google Scholar]
  18. Novick D., Eshhar Z., Rubinstein M. 1982; Monoclonal antibodies to human alpha-interferon and their use for affinity chromatography. Journal of Immunology 129:2244–2247
    [Google Scholar]
  19. Novick D., Eshhar Z., Gigi O., Marks Z., Revel M., Rubinstein M. 1983; Affinity chromatography of human fibroblast interferon (IFN-β1) by monoclonal antibody columns. Journal of General Virology 64:905–910
    [Google Scholar]
  20. Oroszlan S., Bova D., Gilden R. V. 1975; Isolation of mammalian type C RNA virus cross-reactive antigen and antibody by immuno-affinity chromatography. Immunochemistry 12:61–66
    [Google Scholar]
  21. Purcifull D. E. 1966; Some properties of tobacco etch virus and its alkaline degradation products. Virology 29:8–14
    [Google Scholar]
  22. Secher D., Burke D. C. 1980; A monoclonal antibody for large-scale purification of human leukocyte interferon. Nature, London 285:446–450
    [Google Scholar]
  23. Shapiro A. L., Vinuela E., Maizel J. V. JR 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS–polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  24. Sjoberg B. M., Holmgren A. 1973; Purification of thioredoxin from Escherichia coli and bacteriophage T4 by immunosorbent affinity chromatography. Biochimica et biophysica acta 315:176–180
    [Google Scholar]
  25. Sweet C., Stephen J., Smith H. 1974; Purification of influenza viruses using disulphide–linked immunosorbents derived from rabbit antibody. Immunochemistry 11:295–304
    [Google Scholar]
  26. Voller A., Bidwell D. E., Bartlett A. 1979 The Enzyme–labelled Immunosorbent Assay (ELISA) Alexandria: Dynatech Laboratories;
    [Google Scholar]
  27. Weintraub B. D. 1970; Concentration and purification of human chorionic somato–mammotropin by affinity chromatography: application to radioimmunoassay. Biochemical and Biophysical Research Communications 39:83–89
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-2-345
Loading
/content/journal/jgv/10.1099/0022-1317-67-2-345
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error