1887

Abstract

Summary

The isolation of a collection of 44 morphologically T1-like phages is described. It is shown that these phages share some similarity with T1 in terms of cross-inactivation with anti-T1 serum, particle proteins and DNA packaging by the headful process. Virion DNA extracted from these phages was treated with T1 packaging extracts and the reaction mixtures were tested for the formation of infectious phage particles. The packaging efficiencies observed varied from about 1 to 100% of that of virion T1 DNA. Phage λ virion DNA was packaged with an efficiency of between 0.01 and 2% (5 × 10 to 3 × 10 p.f.u./µg DNA), the shorter deleted derivative λ L47 being packaged more efficiently than normal length λ CI857 DNA. Virion DNA from phages T3 and T7 was also packaged at an efficiency similar to that for λ. The packaging of T1 DNA requires the presence of the sequence which initiates headful packaging from a concatemeric precursor. The high efficiency of packaging DNA from some of the T1-like phages may indicate the presence of similar packaging sequences. However, in the case of λ L47, which is known not to contain such a sequence, the DNA packaging reaction must occur by a secondary pathway unrelated to the headful mechanism.

Keyword(s): DNA packaging , pac site and phage T1
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-333
1986-02-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020333.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-333&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages. New York: Interscience;
    [Google Scholar]
  2. Davison P. J., Ramsay N., Ritchie D. A. 1984; In vitro packaging of exogenous phage T1 DNA by extracts of phage-infected E. coli. FEMS Microbiology Letters 21:71–75
    [Google Scholar]
  3. Drexler H. 1970; Transduction by bacteriophage T1. Proceedings of the National Academy of Sciences, U.S.A 66:1083–1088
    [Google Scholar]
  4. Drexler H. 1984; Initiation by bacteriophage T1 of DNA packaging at a site between the P and Q genes of bacteriophage lambda. Journal of Virology 49:754–759
    [Google Scholar]
  5. Drexler H., Christensen J. R. 1979; Transduction of bacteriophage lambda by bacteriophage T1. Journal of Virology 30:543–550
    [Google Scholar]
  6. Dunn J. J., Studier F. W. 1983; Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. Journal of Molecular Biology 166:477–535
    [Google Scholar]
  7. Feiss M., Fisher R., Siegele D. A., Widner W. 1981 Bacteriophage Assembly pp. 213–222 New York: Alan R. Liss;
    [Google Scholar]
  8. Gill G. S., Machattie L. A. 1976; Limited permutations of the nucleotide sequence in bacteriophage T1 DNA. Journal of Molecular Biology 104:505–515
    [Google Scholar]
  9. Hausmann R. 1976; Bacteriophage T7 genetics. Current Topics in Microbiology and Immunology 75:77–110
    [Google Scholar]
  10. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39–56
    [Google Scholar]
  11. Hohn B. 1975; DNA as substrate for packaging into bacteriophage lambda, in vitro. Journal of Molecular Biology 98:93–106
    [Google Scholar]
  12. Korsten K. H., Tomkiewicz C., Hausmann R. 1979; The strategy of infection as a criterion for phylogenetic relationships of non-coli phages morphologically similar to phage T7. Journal of General Virology 43:57–73
    [Google Scholar]
  13. Liebeschuetz J., Davison P. J., Ritchie D. A. 1985; A coupled in vitro system for the formation and packaging of concatemeric phage T1 DNA. Molecular and General Genetics 200:451–457
    [Google Scholar]
  14. Loenen W. A. M., Brammar W. J. 1980; A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 20:249–259
    [Google Scholar]
  15. Machattie L. A., Gill S. 1977; DNA maturation by the ‘headful’ mode in bacteriophage T1. Journal of Molecular Biology 110:441–465
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Martin D. T. M., Adair C. A., Ritchie D. A. 1976; Polypeptides specified by bacteriophage T1. Journal of General Virology 33:309–319
    [Google Scholar]
  18. Mertens H., Hausmann R. 1982; Coliphage BA14: a new relative of phage T7. Journal of General Virology 62:331–341
    [Google Scholar]
  19. Ramsay N., Ritchie D. A. 1980; A physical map of the permuted genome of bacteriophage T1. Molecular and General Genetics 179:669–675
    [Google Scholar]
  20. Ramsay N., Ritchie D. A. 1982; Restriction endonuclease analysis of variants of phage T1: correlation of the physical and genetic maps. Virology 121:420–425
    [Google Scholar]
  21. Ramsay N., Ritchie D. A. 1983; Uncoupling of initiation site cleavage from subsequent headful cleavages in bacteriophage T1 DNA packaging. Nature, London 301:264–266
    [Google Scholar]
  22. Ritchie D. A., Joicey D. H. 1978; Formation of concatemeric DNA as an intermediate in the replication of bacteriophage T1 DNA molecules. Journal of General Virology 41:609–622
    [Google Scholar]
  23. Ritchie D. A., Davison R. J., Liebeschuetz J. 1984; In vitro replication, recombination and packaging of phage T1 DNA molecules. In Proceedings, 5 th International Symposium on Metabolism and Enzymology of Nucleic Acids pp. 249–257 Edited by Zelinka J., Balan J. Bratislava: Institute of Molecular Biology, Slovak Academy of Sciences;
    [Google Scholar]
  24. Streisinger G., Emrich J., Stahl M. N. 1967; Chromosome structure in phage T4. III. Terminal redundancy and length determination. Proceedings of the National Academy of Sciences, U.S.A. 57:292–295
    [Google Scholar]
  25. Strobel E., Behnisch W., Schmieger H. 1984; In vitro packaging of mature phage DNA by Salmonella phage P22. Virology 133:158–165
    [Google Scholar]
  26. Trautner T. A. 1960; Genetische und physiologische Beziehungen zwischen den Bakteriophagen T1 und D20. Zeitschrift fur Vererbungslehre 91317–324
    [Google Scholar]
  27. Tye B.-K., Huberman J. A., Botstein D. 1974; Non-random circular permutation of phage P22 DNA. Journal of Molecular Biology 85:501–532
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-2-333
Loading
/content/journal/jgv/10.1099/0022-1317-67-2-333
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error