1887

Abstract

Summary

Both whole virus particles and isolated VP1 of foot-and-mouth disease virus type O1 induce neutralizing antibodies. Results obtained with pigs vaccinated with either isolated VP1 or intact particles and subsequently challenged show that neutralizing activity induced by intact virus correlates well with protection in pigs, whereas neutralizing activity induced by isolated VP1 confers little or no protection. Further evidence suggests that the epitope responsible for the induction of neutralizing antibodies by VP1 is located at the C-terminal end of the protein between residues 200 and 210.

Keyword(s): FMDV , neutralizing activity and VP1
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-289
1986-02-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020289.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-289&mimeType=html&fmt=ahah

References

  1. Bachrach H. L., Moore D. M., Mckercher P. D., Polatnick J. 1975; Immune and antibody responses to an isolated capsid protein of FMDV. Journal of Immunology 115:1636–1641
    [Google Scholar]
  2. Barteling S. J. 1974; Use of polyethylene glycol treated serum for the production of foot-and-mouth disease virus growing in BHK-suspended cell cultures. Bulletin. Office international des epizooties 81:1243–1254
    [Google Scholar]
  3. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against FMDV by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature. London 298:30–34
    [Google Scholar]
  4. Boothroyd J. C., Harris T. J. R., Rowlands D. J., Lowe P. A. 1982; The nucleotide sequence of cDNA coding for the structural proteins of foot-and-mouth disease virus. Gene 17:153–161
    [Google Scholar]
  5. Cartwright B., Morrell D. J., Brown F. 1982; Nature of the antibody response to the foot-and-mouth disease virus particle, its 12S protein subunit and the isolated immunizing polypeptide VP1. Journal of General Virology 63:375–381
    [Google Scholar]
  6. Cheung A., Delamarter J., Weiss S., Kupper H. 1983; Comparison of the major antigenic determinants of different serotypes of foot-and-mouth disease virus. Journal of Virology 48:451–459
    [Google Scholar]
  7. De leeuw P. W., Tiessink J. W. A., Van Bekkum I. G. 1979; The challenge of vaccinated pigs with foot-and- mouth disease virus. Zentralblatt für Veterinarmedizin, reihe B 26:98–109
    [Google Scholar]
  8. Geysen H. M., Meloen R. H., Barteling S. I. 1984; Peptide synthesis used to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, U.S.A 81:3998–4002
    [Google Scholar]
  9. Geysen H. M., Barteling S. J., Meloen R. H. 1985; Small peptides induce antibodies with a sequence and structural requirement for binding antigen comparable to antibodies raised against the native protein. Proceedings of the National Academy of Sciences, U.S.A 82:178–182
    [Google Scholar]
  10. Kleid D. G., Yansura D., Small B., Dowbenko D., Moore D. M., Grubman J. M., Morgan D. O., Robertson B. H., Bachrach H. L. 1981; Cloned viral protein vaccine for foot-and-mouth disease. Responses in cattle and swine. Science 214:1125–1128
    [Google Scholar]
  11. Kurz C., Forss S., Küpper H., Strohmaier K., Schaller H. 1981; Nucleotide sequence and corresponding amino acid sequence of the gene for the major antigen of foot-and-mouth disease virus. Nucleic Acids Research 9:1919–1931
    [Google Scholar]
  12. Laporte J. M., Grosclaude J., Wantyghem J., Bernard S., Rouze P. 1973; Neutralisation en culture cellulaire du pouviour infectieux du virus de la fièvre aphteuse par des sérums provenant de pores immunisés à l’aide d'une protéine virale purifiée. Comptes rendus hebdomadaires des séances de l’Académie des sciences, série D 276:3399–3402
    [Google Scholar]
  13. Mckercher P. D., Moore D. M., Morgan D. O., Robertson B. H., Callis J. J., Kleid D. G., Shire S., Yansura D., Small B. 1983; Genetically-engineered polypeptide antigen for foot-and-mouth disease: a dose response in cattle. Report of the 25th Session of the European Commission for the Control of Foot-and-Mouth Disease (FAO, Rome; 12–15 April 1983)
    [Google Scholar]
  14. Meloen R. H., Barteling S. J. 1986; Epitope mapping of the outer structural protein VP1 of three different serotypes of Foot-and-Mouth Disease virus. Virology (in press)
    [Google Scholar]
  15. Meloen R. H., Briaire J. 1980; A study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12S subunits with antisera against the structural proteins. Journal of General Virology 51:107–116
    [Google Scholar]
  16. Meloen R. H., Rowlands D. J., Brown F. 1979; Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polio viruses. Journal of General Virology 45:761–763
    [Google Scholar]
  17. Meloen R. H., Briaire J., Woortmeyer R. J., Van Zaane D. 1983; The main antigenic determinant detected by neutralizing monoclonal antibodies on the intact foot-and-mouth disease virus particle is absent from isolated VP1. Journal of General Virology 64:1193–1198
    [Google Scholar]
  18. Parry N. R., Ouldridge E. J., Barnett P. V., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1985; Identification of neutralizing epitopes of foot-and-mouth disease virus. In Vaccines 85 pp. 211–216 Edited by Lerner R. A. Chanock R. M. Brown F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Pereira H. 1977; International symposium on foot-and-mouth disease. Developments in Biological Standardization 35:167–174
    [Google Scholar]
  20. Rowlands D. J., Sangar D. V., Brown F. 1971; Relationship of the antigenic structure of foot-and-mouth disease virus to the process of infection. Journal of Virology 13:85–93
    [Google Scholar]
  21. Rueckert R. R. 1976; On the structure and morphogenesis of picornaviruses. In Comprehensive Virology vol 6 pp. 131–213 Edited by Fraenkel-Conrat H. Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  22. Strohmaier K., Adam K. H. 1974; Comparative electrophoretic studies of foot-and-mouth disease virus proteins. Journal of General Virology 22:105–114
    [Google Scholar]
  23. Strohmaier K., Franze R., Adam K. H. 1982; Location and characterization of the antigenic portion of FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  24. Wild T. F., Brown F. 1967; Nature of the inactivating action of trypsin on foot-and-mouth disease virus. Journal of General Virology 1:247–250
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-2-289
Loading
/content/journal/jgv/10.1099/0022-1317-67-2-289
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error