1887

Abstract

Summary

Antigenic characteristics of nine mumps virus strains were determined by immunofluorescence and radioimmunoprecipitation assay (RIPA) using a collection of 44 monoclonal antibodies. These antibodies were directed against five different structural components of mumps virus, the haemagglutinin-neuraminidase (HN), fusion (F), matrix (M), phospho- (P) and nucleocapsid (NP) proteins. The nine mumps virus strains could be divided into two groups according to their antigenic characteristics. One group included two strains isolated more than a decade ago and the Jeryl Lynn vaccine strain. These three strains reacted with a wider range of monoclonal antibodies than the second group of six recently isolated strains of different geographical origin. In the F, M and P proteins variations were only found in single antigenic determinants. In the HN and NP components, RIPA revealed variations in three and seven determinants respectively. The Jeryl Lynn vaccine strain showed a unique lack of reaction with one anti-HN antibody clone in the RIPA.

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1986-02-01
2023-02-06
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References

  1. Buynak E. B., Hilleman M. R. 1966; Live attenuated mumps virus vaccine. 1. Vaccine development. Proceedings of the Society for Experimental Biology and Medicine 123:768–775
    [Google Scholar]
  2. Cantell K. 1961; Mumps virus. Advances in Virus Research 8:123–164
    [Google Scholar]
  3. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  4. Kristensson K., Örvell C., Leestma J., Norrby E. 1983; Sendai virus infection in the brains of mice: distribution of viral antigens studied with monoclonal antibodies. Journal of Infectious Diseases 147:297–301
    [Google Scholar]
  5. McCarthy M., Johnson R. T. 1980; A Comparison of the structural polypeptides of five strains of mumps virus. Journal of General Virology 46:15–27
    [Google Scholar]
  6. McCarthy M., Jubelt B., Fay D. B., Johnson R. T. 1980; Comparative studies of five strains of mumps virus in vitro and in neonatal hamsters: evaluation of growth, cytopathogenicity and neurovirulence. Journal of Medical Virology 5:1–15
    [Google Scholar]
  7. Mufson M. A., Ӧrvell C., Rafnar B., Norrby E. 1985; Two distinct sybtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  8. Norrby E. 1962; Hemagglutination by measles virus. IV. A simple procedure for production of high potency antigen for hemagglutination inhibition (HI) tests. Proceedings of the Society for Experimental Biology and Medicine 111:814–818
    [Google Scholar]
  9. Norrby E. 1985; Variability of antigen epitopes of monotypic viruses. In Proceedings of the Fourth International Symposium on Rapid Methods and Automation in Microbiology and Immunology Edited by Habermehl K. O. Heidelberg: Springer-Verlag; (in press)
    [Google Scholar]
  10. Norrby E., Sheshberadaran H., Mccullough K., Carpenter W. C., Ӧrvell C. 1985; Is rinderpest virus the archevirus of the morbillivirus genus?. Intervirology 23:228–232
    [Google Scholar]
  11. Ӧrvell C. 1976; Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutination-inhibiting and neuraminidase-inhibiting antibodies. Acta pathologica et microbiologica scandinavica (Section B, Microbiology) 84:441–450
    [Google Scholar]
  12. Ӧrvell C. 1984; The reactions of monoclonal antibodies with structural proteins of mumps virus. Journal of Immunology 132:2622–2629
    [Google Scholar]
  13. Ӧrvell C., Norrby E. 1980; Immunological relationships between homologous structural polypeptides of measles and canine distemper virus. Journal of General Virology 50:231–245
    [Google Scholar]
  14. Ӧrvell C., Norrby E. 1985; Antigenic structure of paramyxoviruses. In Immunochemistry of Viruses. The Basis for Serodiagnosis and Vaccines pp. 241–264 Edited by van Regenmortel M. H. V. Neurath A. R. New York: Elsevier;
    [Google Scholar]
  15. Server A. C., Merz D. C., Waxham M. N., Wolinsky J. S. 1982; Differentiation of mumps virus strains with monoclonal antibody to the HN glycoprotein. Infection and Immunity 35:179–186
    [Google Scholar]
  16. Sheshberadaran H., Chen S. N., Norrby E. 1983; Monoclonal antibodies against five structural components of measles virus. I. Characterization of antigenic determinants on nine strains of measles virus. Virology 128:341–353
    [Google Scholar]
  17. Webster R. G., Laver W. G., Air G. M., Schild G. C. 1982; Molecular mechanisms of variation in influenza viruses. Nature, London 296:115–121
    [Google Scholar]
  18. Wolinsky J. S., Waxham M. N., Server A. C. 1985; Protective effects of glycoprotein-specific monoclonal antibodies on the course of experimental mumps virus meningoencephalitis. Journal of Virology 53:727–734
    [Google Scholar]
  19. Yewdell J. W., Gerhard W. 1981; Antigenic characterization of viruses by monoclonal antibodies. Annual Review of Microbiology 35:185–206
    [Google Scholar]
  20. Zollar L. M., Mufson M. A. 1970; Acute parotitis associated with parainfluenza 3 virus infection. American Journal of Diseases of Children 119:147–148
    [Google Scholar]
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