1887

Abstract

Summary

A virus-specific antigen was extracted from brains of rats and from MDCK cells infected with Borna disease (BD) virus and purified to homogeneity by immunoaffinity chromatography and HPLC. The antigen consists of two components which are almost equal in size (38000 mol. wt.), and it forms aggregates in its native form. The virus specificity of the two antigenic entities was confirmed by immunoblots with convalescent serum and monoclonal antibodies. Immunofluorescent staining with monoclonal antibodies and a hyperimmune serum prepared against the purified antigen showed the intranuclear fluorescence typical for BD virus-infected cells.

Keyword(s): antigen , BD virus and nuclear
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/content/journal/jgv/10.1099/0022-1317-67-2-235
1986-02-01
2024-12-07
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References

  1. Axen R., Ernback S. 1971; Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers. European Journal of Biochemistry 18:351–360
    [Google Scholar]
  2. Becht H., Malole B. 1975; Comparative evaluation of different fixation procedures and different coupling reagents for the demonstration of influenza virus-specific antibodies by indirect haemagglutination test. Medical Microbiology and Immunology 162:43–53
    [Google Scholar]
  3. Danner K. 1976; Labordiagnose der Borna’schen Krankheit. Über den Nachweis von Antikörpern im Zentralnervensystem. Zentralblatt für Veterinärmedizin B23:856–867
    [Google Scholar]
  4. Fazekas De St. Groth, S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:11–20
    [Google Scholar]
  5. Herzog S., Rott R. 1980; Replication of Borna disease virus in cell cultures. Medical Microbiology and Immunology 168:153–158
    [Google Scholar]
  6. Jensenius J. C., Andersen I., Hau J., Crone M., Koch C. 1981; Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG. Journal of Immunological Methods 46:63–68
    [Google Scholar]
  7. Kao M., Ludwig H., Gosztonyi G. 1984; Adaptation of Borna disease virus to the mouse. Journal of General Virology 65:1845–1849
    [Google Scholar]
  8. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Ludwig H., Becht H. 1977; Borna disease-a summary of our present knowledge. In Slow Virus Infections of the Central Nervous System pp. 75–83 Edited by Ter Meulen V. Katz M. New York, Heidelberg & Berlin: Springer-Verlag;
    [Google Scholar]
  11. Ludwig H., Becht H., Groh L. 1973; Borna disease (BD), a slow virus infection: biological properties of the virus. Medical Microbiology and Immunology 158:275–289
    [Google Scholar]
  12. Maher P., Molday R. S. 1977; Binding of concanavalin A to Ricinus communis agglutinin and its application in cell-surface labeling studies. FEBS Letters 84:391–395
    [Google Scholar]
  13. Narayan O., Herzog S., Frese K., Scheefers H., Rott R. 1983; Behavioral disease in rats caused by immunopathological responses to persistent Borna virus in the brain. Science 220:1401–1403
    [Google Scholar]
  14. Porath J., Sundberg L. 1972; High capacity chemoabsorbents for protein immobilisation. Nature New Biology 238:261–262
    [Google Scholar]
  15. Talbot P. J., Knobler R. L., Buchmeier M. J. 1984; Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus. Journal of Immunological Methods 73:177–188
    [Google Scholar]
  16. Towbin H., Staehelin T., Cordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gel to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  17. Von Sprockhoff H. 1954; Untersuchungen über die Komplement-Bindungsreaktion bei der Borna’ schen Krankheit. Zentralblatt fur Veterinarmedizin 1:494–530
    [Google Scholar]
  18. Wagner K., Ludwig H., Paulsen J. 1968; Fluoreszenzserologischer Nachweis von Borna-Virus Antigen. Berliner und Münchener tierärztliche Wochenschrift 81:395–396
    [Google Scholar]
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