1887

Abstract

Summary

Infection of insect vector cells with the plant rhabdovirus potato yellow dwarf, serotype SYDV, was inhibited when the lysosomotropic substances chloroquine or ammonium chloride were present in the culture medium. Chloroquine inhibited infection totally at 1 m, whereas inhibition by NHCl was maximum at 14 m, where it was almost complete; lower concentrations of NHCl or, surprisingly, higher concentrations were less inhibitory. The number of cells infected at 25 m-NHCl was the same as that infected in untreated controls. Binding of SYDV to the surfaces of cells was unimpaired by chloroquine. Sensitivity of SYDV infection to the lysosomotropic substances was observed only if the substances were administered during 150 min after inoculation. The inhibitory effect was abolished when the substances were removed from the medium. These results suggest that SYDV infection of the vector cells occurs by adsorptive endocytosis and has a lysosomal phase.

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/content/journal/jgv/10.1099/0022-1317-67-12-2775
1986-12-01
2022-01-26
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References

  1. Adam G., Hsu H. T. 1984; Comparison of structural proteins from two potato yellow dwarf viruses. Journal of General Virology 65:991–994
    [Google Scholar]
  2. Adam G., Gaedigk K., Mundry K.-W. 1983; Alterations of a plant rhabdovirus during successive mechanical transfers. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 90:28–35
    [Google Scholar]
  3. Black L. M. 1941; Specific transmission of varieties of potato yellow dwarf virus by related insects. American Potato Journal 18:231–233
    [Google Scholar]
  4. Chiu R. J., Black L. M. 1967; Monolayer cultures of insect cell lines and their inoculation with a plant virus. Nature, London 215:1076–1078
    [Google Scholar]
  5. De Duve C., De Barsy T., Poole B., Trouet A., Tulkens P., Van Hoof F. 1974; Lysosomotropic agents. Biochemical Pharmacology 23:2495–2531
    [Google Scholar]
  6. Falk B. W., Weathers L. G. 1983; Comparisons of potato yellow dwarf virus serotypes. Phytopathology 73:81–85
    [Google Scholar]
  7. Gaedigk K., Adam G., Mundry K.-W. 1986; The spike protein of potato yellow dwarf virus and its functional role in the infection of insect vector cells. Journal of General Virology 67:2763–2773
    [Google Scholar]
  8. Geraldes A., Valdeira M. L. 1985; Effect of chloroquine on African swine fever virus infection. Journal of General Virology 66:1145–1148
    [Google Scholar]
  9. Helenius A., Marsh M., White J. 1982; Inhibition of Semliki Forest virus penetration by lysosomotropic weak bases. Journal of General Virology 58:47–61
    [Google Scholar]
  10. Hsu H. T., Black L. M. 1973; Inoculation of vector cell monolayers with potato yellow dwarf virus. Virology 52:187–198
    [Google Scholar]
  11. Hsu H. T., Nuss D. L., Adam G. 1983; Utilization of insect tissue culture in the study of the molecular biology of plant viruses. In Current Topics in Vector Research vol 1 pp. 189–214 Edited by Harris K. F. New York: Praeger Publishers;
    [Google Scholar]
  12. Huang R. T. C., Dietsch E., Rott R. 1985; Further studies on the role of neuraminidase and the mechanism of low pH dependence in influenza virus-induced membrane fusion. Journal of General Virology 66:295–301
    [Google Scholar]
  13. Liu H. Y., Black L. M. 1976; Improvements in establishing and growing leafhopper cell cultures. Proceedings of the American Phytopathological Society 3:234–235
    [Google Scholar]
  14. Marsh M., Helenius A. 1980; Adsorptive endocytosis of Semliki Forest virus. Journal of Molecular Biology 142:439–454
    [Google Scholar]
  15. Matlin K. S., Regoio H., Helenius A., Simons K. 1981; Infectious entry pathway of influenza virus in a canine kidney cell line. Journal of Cell Biology 91:601–613
    [Google Scholar]
  16. Matlin K. S., Reggio H., Helenius A., Simons K. 1982; Pathway of vesicular stomatitis virus entry leading to infection. Journal of Molecular Biology 156:609–631
    [Google Scholar]
  17. Miller D. K., Lenard J. 1980; Inhibition of vesicular stomatitis virus infection by spike glycoprotein. Evidence for an intracellular, G protein-requiring step. Journal of Cell Biology 84:430–437
    [Google Scholar]
  18. Ohkuma S., Poole B. 1978; Fluorescence probe measurement of the intralysosomal pH and the perturbation of pH by varying agents. Proceedings of the National Academy of Sciences, U.S.A. 75:3327–3331
    [Google Scholar]
  19. Superti F., Derer M., Tsiang H. 1984; Mechanism of rabies virus entry into CER cells. Journal of General Virology 65:781–789
    [Google Scholar]
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