Protoplasts from Nicotiana clevelandii were infected with velvet tobacco mottle virus (VTMoV) using inocula containing 2 µg/ml poly-l-ornithine (PLO), 8 µg/ml VTMoV and 0.02 m-potassium citrate pH 5.0. Typically about 47% of the inoculated protoplasts become infected. The amount of virus particles bound to inoculated protoplasts was increased by a decrease in pH, an increase in PLO concentration or an increase in virus concentration. The one-step growth curve of VTMoV in N. clevelandii protoplasts showed that, although large amounts of virus were synthesized, the multiplication was slower than that published for other viruses. Alpha-amanitin (50 µg/ml) had no effect on the rate of accumulation of virus coat protein or on the synthesis of either RNA species of VTMoV when added to cultures immediately after inoculation.
ChuP. W. G.,
FranckiR. I. B.,
RandlesJ. W.1983; Detection, isolation, and characterization of high molecular weight double-stranded RNAs in plants infected with velvet tobacco mottle virus. Virology 126:480–492
ClarkM. F.,
AdamsA. N.1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
GouldA. R.1981; Studies on encapsidated viroid-like RNA. II. Purification and characterization of a viroid-like RNA associated with velvet tobacco mottle virus (VTMoV). Virology 108:123–133
GouldA. R.,
HattaT.1981; Studies on encapsidated viroid-like RNA. III. Comparative studies on RNAs isolated from velvet tobacco mottle virus (VTMoV) and solanum nodiflorum mottle virus (SNMV). Virology 109:137–147
GouldA. R.,
FranckiB. R. I.,
RandlesJ. W.1981; Studies on encapsidated viroid-like RNA. IV. Requirement for infectivity and specificity of two RNA components from velvet tobacco mottle virus. Virology 110:420–426
JonesA. T.,
MayoM. A.1983; Interaction of lucerne transient streak virus and the viroid-like RNA-2 of Solanum nodiflorum mottle virus. Journal of General Virology 64:1771–1774
JonesA. T.,
MayoM. A.,
DuncanG. H.1983; Satellite-like properties of small circular RNA molecules in particles of lucerne transient streak virus. Journal of General Virology 64:1167–1173
KassanisB.,
WhiteR. F.1974; A simplified method of obtaining tobacco protoplasts for infection with tobacco mosaic virus. Journal of General Virology 24:447–452
KiberstisP. A.,
ZimmernD.1984; Translational strategy of Solanum nodiflorum mottle virus RNA: synthesis of a coat protein precursor in vitro and in vivo
. Nucleic Acids Research 12:933–943
MatthewsR. E. F.1979; Classification and nomenclature of viruses. Third report of the International Committee on Taxonomy of Viruses. Intervirology 12:132–296
MühlbachH.-P.,
SangerH. L.1977; Multiplication of cucumber pale fruit viroid in inoculated tomato leaf protoplasts. Journal of Genera! Virology 35:377–386
QiuB. S.,
TienP.1982; Infection of barley protoplasts with barley stripe mosaic virus detected by immunoperoxidase and complementary DNA probe. Acta microbiologica sinica 22:321–326
RandlesJ. M.,
DaviesC.,
HattaT.,
GouldA. R.,
FranckiR. I. B.1981; Studies on encapsidated viroid-like RNA. I. Characterization of velvet tobacco mottle virus. Virology 108:111–122
SymonsR. H.,
HutchingC. J.,
KeeseP.,
VisvaderJ. E.,
McinnesJ.1984; On the mechanism of replication of viroids and virusoids. In Abstracts of The Sixth International Congress of Virology P47–13 p 74
TakebeI.,
OtsukiY.1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences, U.S.A. 64:843–848
WeinmannR.,
RoederG.R.1974; Role of DNA-dependent RNA polymerase III in the transcription of the tRNA and 5s RNA genes. Proceedings of the National Academy of Sciences, U.S.A. 71:1790–1794