RT Journal Article SR Electronic(1) A1 Jian-Guo, Wu A1 Wen-Jun, Lu A1 Po, TienYR 1986 T1 Multiplication of Velvet Tobacco Mottle Virus in Nicotiana clevelandii Protoplasts Is Resistant to α-Amanitin JF Journal of General Virology, VO 67 IS 12 SP 2757 OP 2762 DO https://doi.org/10.1099/0022-1317-67-12-2757 PB Microbiology Society, SN 1465-2099, AB Summary Protoplasts from Nicotiana clevelandii were infected with velvet tobacco mottle virus (VTMoV) using inocula containing 2 µg/ml poly-l-ornithine (PLO), 8 µg/ml VTMoV and 0.02 m-potassium citrate pH 5.0. Typically about 47% of the inoculated protoplasts become infected. The amount of virus particles bound to inoculated protoplasts was increased by a decrease in pH, an increase in PLO concentration or an increase in virus concentration. The one-step growth curve of VTMoV in N. clevelandii protoplasts showed that, although large amounts of virus were synthesized, the multiplication was slower than that published for other viruses. Alpha-amanitin (50 µg/ml) had no effect on the rate of accumulation of virus coat protein or on the synthesis of either RNA species of VTMoV when added to cultures immediately after inoculation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-12-2757