1887

Abstract

Summary

Protoplasts from were infected with velvet tobacco mottle virus (VTMoV) using inocula containing 2 µg/ml poly--ornithine (PLO), 8 µg/ml VTMoV and 0.02 -potassium citrate pH 5.0. Typically about 47% of the inoculated protoplasts become infected. The amount of virus particles bound to inoculated protoplasts was increased by a decrease in pH, an increase in PLO concentration or an increase in virus concentration. The one-step growth curve of VTMoV in protoplasts showed that, although large amounts of virus were synthesized, the multiplication was slower than that published for other viruses. Alpha-amanitin (50 µg/ml) had no effect on the rate of accumulation of virus coat protein or on the synthesis of either RNA species of VTMoV when added to cultures immediately after inoculation.

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/content/journal/jgv/10.1099/0022-1317-67-12-2757
1986-12-01
2021-10-20
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