When particles of rice dwarf virus were partially disrupted by treatment with EDTA, genome RNA was extruded. Electron microscope observations of these particles suggested that the genome RNA is packed within the virus particle as a supercoiled structure. Genome RNA, extracted using a newly devised two-step extraction method, appeared to consist of supercoiled segments of RNA complexed with protein.
DunnebackeT. H.,
KleinschmidtA. K.1967; Ribonucleic acid from reovirus as seen in protein monolayers by electron microscopy. Zeitschrift für Naturforschung 22b:159–164
LewandowskiL. I.,
KalmakoffJ.,
TanadaY.1969; Characterization of a ribonucleic acid polymerase activity associated with purified cytoplasmic polyhedrosis virus of the silkworm Bombyx mori
. Journal of Virology 4:857–865
MiuraK.,
Fujii-KawataI.,
SakakiT.,
FukeM.,
KawaseS.1968; Double-stranded ribonucleic acid from cytoplasmic polyhedrosis virus of the silkworm. Journal of Virology 2:1211–1222
SatoT.,
KyogokuY.,
HiguchiS.,
MitsuiY.,
HtakaY.,
TsuboiM.,
MiuraK.1966; A preliminary investigation on the molecular structure of rice dwarf virus ribonucleic acid. Journal of Molecular Biology 16:180–190
SharpP.A.,
SugdenB.,
SambrookJ.1973; Detection of two restriction endonuclease activities in Haemophilus parainfluenza using analytical agarose–ethidium bromide electrophoresis. Biochemistry 12:3055–3063
ShimotohnoK.,
MiuraK.1973a; Single-stranded RNA synthesis in vitro by the RNA polymerase associated with cytoplasmic polyhedrosis virus containing double-stranded RNA. Journal of Biochemistry 74:117–125
SugiuraM.,
MiuraK.1977; Transcription of double-stranded RNA by Escherichia coli DNA-dependent RNA polymerase. European Journal of Biochemistry 73:179–184
UyedaI.,
ShikataE.1984; Characterization of RNAs synthesized by the virion-associated transcriptase of rice dwarf virus in vitro
. Virus Research 1:527–532
VollenweiderH. J.,
SogoJ. M.,
KollerTH.1975; A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules. Proceedings of the National Academy of Sciences, U.S.A. 72:83–87