RT Journal Article SR Electronic(1) A1 Rosztóczy, I.YR 1986 T1 Study of the in vivo Priming Effect of Interferon in Mice JF Journal of General Virology, VO 67 IS 12 SP 2731 OP 2737 DO https://doi.org/10.1099/0022-1317-67-12-2731 PB Microbiology Society, SN 1465-2099, AB Summary Intramuscular injection of mice with 2000 IU/g partially purified murine interferon (IFN) alpha/beta 3 h before the induction of IFN by intraperitoneally administered 3 µg/g poly rI:rC enhanced early IFN production. The differences between the serum IFN levels of IFN-pretreated and control animals were about 10-fold during the first 2 to 3 h of in vivo IFN production. In later stages these differences tended to decrease, and from 8 h post-induction they disappeared. IFN exerted its in vivo priming activity equally after intravenous, intraperitoneal or intramuscular injection. A similar enhancement of IFN production was observed when it was induced by poly rI:rC administered either intraperitoneally or intramuscularly. The duration of IFN pretreatment influenced the establishment of the in vivo primed state. Following administration of 2000 IU/g murine IFN alpha/beta intramuscularly, maximal priming developed after 3 h, and no primed IFN response was detected when the inoculation of IFN preceded inducer administration by 12 h or more. The manifestation of in vivo priming was optimal when 1500 to 3000 IU/g pretreating doses of IFN were applied. Reduction of the amount of injected IFN below this level markedly decreased priming, indicating a time- and dose-dependent induction of priming in vivo., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-12-2731