1887

Journal of General Virology header logo

Volume 67, Issue 12

Human Cytomegalovirus Persistent Infection in a Human Central Nervous System Cell Line: Production of a Variant Virus with Different Growth Characteristics Free

Abstract

Summary

The susceptibility of human central nervous system cell lines to human cytomegalovirus (HCMV) and the fate of infected cultures were studied. Significant amounts of infectious progeny virus were produced in 118MGC glioma and IMR-32 neuroblastoma, but not in KGC oligodendroglioma cells when the cultures were infected with wild-type virus (HCMVwt) at an m.o.i. of 10 p.f.u. per cell. Further passage of infected 118MGC cells resulted in the establishment of a long-term persistent infection. This infection, designated 118MGC/Towne, continuously produced infectious virus (HCMVpi) with titres ranging from 10 to 10 p.f.u./10 cells up to 360 days post-infection (corresponding to 50 subcultures). Since no temperature-sensitive mutants, defective interfering particles or interferon-like activity were found in the 118MGC/Towne cultures, maintenance of the persistent infection seemed to be due to a balance between the release of infectious virus and the growth of uninfected cells. The HCMVpi produced in long-term persistently infected cultures was shown to be different from the HCMVwt originally used to infect by the following characteristics: (i) HCMVpi replicated slowly and yielded lower amounts of progeny virus than HCMVwt; (ii) HCMVpi induced a 73000 mol. wt. immediate early protein that was not synthesized in HCMVwt-infected cells; (iii) HCMVpi had a different DNA structure from that of HCMVwt. These results suggest that HCMVpi is a slower growing variant of HCMVwt and probably plays an important role in the maintenance of the persistent infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CNS cell line , HCMV , persistent infection and variant virus
© Journal of General Virology 1986
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-12-2605
1986-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/12/JV0670122605.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-12-2605&mimeType=html&fmt=ahah

References

  1. Berenberg W., Nankervis G. 1970; Long-term follow-up of cytomegalic inclusions disease of infancy. Pediatrics 46:403–410
     [Google Scholar]
  2. Blanton R. A., Tevethia M. J. 1981; Immunoprecipitation of virus-specific immediate-early and early polypeptides from cells lytically infected with human cytomegalovirus strain AD169. Virology 112:262–273
     [Google Scholar]
  3. Dal Canto M. C., Rabinowitz S. G. 1981; Murine central nervous system infection by a viral temperature-sensitive mutant. American Journal of Pathology 102:412–426
     [Google Scholar]
  4. Dauenhauer S. A., Robinson R. A., O’callaghan D. J. 1982; Chronic production of defective-interfering particles by hamster embryo cultures of herpesvirus persistently infected and oncogenically transformed cells. Journal of General Virology 60:1–14
     [Google Scholar]
  5. Furukawa T. 1979; Persistent infection with human cytomegalovirus in a lymphoblastoid cell line. Virology 94:214–218
     [Google Scholar]
  6. Furukawa T. 1984; A variant of human cytomegalovirus derived from a persistently infected culture. Virology 137:191–194
     [Google Scholar]
  7. Gibson W. 1981; Immediate-early proteins of human cytomegalovirus strains AD169, Davis, and Towne differ in electrophoretic mobility. Virology 112:350–354
     [Google Scholar]
  8. Hansuaw J. B., Scheiner A. P., Moxley A. W., Gaev L., Abel V., Scheiner B. 1976; School failure and deafness after “silent” congenital cytomegalovirus infection. New England Journal of Medicine 295:468–470
     [Google Scholar]
  9. Ho M. 1982; Congenital and perinatal human cytomegalovirus infection. In Cytomegalovirus: Biology and Infection pp 139–141 New York & London: Plenum Press;
     [Google Scholar]
  10. Jeang K.-T., Gibson W. 1980; A cycloheximide-enhanced protein in cytomegalovirus-infected cells. Virology 107:362–374
     [Google Scholar]
  11. McCracken G. J. JR, Shinefield H. R., Cobb K., Rausen A. R., Dische M. R., Eichenwald H. F. 1969; Congenital cytomegalic inclusion disease. A longitudinal study of 20 patients. American Journal of Diseases of Children 117:522–539
     [Google Scholar]
  12. Miyake E. 1979; Establishment of human oligodendroglial cell line. Acta neuropathologica 46:51–55
     [Google Scholar]
  13. Mocarski E. S., Stinski M. F. 1979; Persistence of the cytomegalovirus genome in human cells. Journal of Virology 31:761–775
     [Google Scholar]
  14. Murray K., Murray N. E. 1975; Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli . Journal of Molecular Biology 115:249–255
     [Google Scholar]
  15. Ponten J., Macintyre E. H. 1968; Long term culture of normal and neoplastic human glia. Acta pathologica, microbiologica et immunologica scandinavica 74:465–486
     [Google Scholar]
  16. Preble O. T., Youngner J. S. 1975; Temperature-sensitive viruses and the etiology of chronic and inapparent infections. Journal of Infectious Diseases 131:467–473
     [Google Scholar]
  17. Ramirez M. L., Virmani M., Garon C., Rosenthal L. J. 1979; Defective virion of human cytomegalovirus. Virology 96:311–314
     [Google Scholar]
  18. Rapp F. 1980; Persistence and transmission of cytomegalovirus. In Comprehensive Virology vol 16 pp 191–232 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
     [Google Scholar]
  19. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
     [Google Scholar]
  20. Schneider J. F., Carp L. R., Belisle E. H., Rue C. E. 1972; The response of murine nerve tissue culture to murine cytomegalovirus. Acta neuropathologica 21:204–212
     [Google Scholar]
  21. Sekellick M. J., Marcus P. I. 1978; Persistent infection. I. Interferon-inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 85:175–186
     [Google Scholar]
  22. Sekellick M. J., Marcus P. I. 1979; Persistent infection. II. Interferon-inducing temperature-sensitive mutants as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 95:36–47
     [Google Scholar]
  23. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  24. Stenberg R. M., Stinski M. F. 1985; Autoregulation of the human cytomegalovirus major immediate early gene. Journal of Virology 56:676–682
     [Google Scholar]
  25. Stinski M. F., Mocarski E. S., Thomsen D. R. 1979; DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. Journal of Virology 31:231–239
     [Google Scholar]
  26. Talbot P., Almeida J. D. 1977; Human cytomegalovirus: purification of enveloped virions and dense bodies. Journal of General Virology 36:345–349
     [Google Scholar]
  27. Tanaka J., Yabuki Y., Hatano M. 1981; Evidence for early membrane antigens in cytomegalovirus-infected cells. Journal of General Virology 53:157–161
     [Google Scholar]
  28. Tanaka J., Ogura T., Kamiya S., Sato H., Yoshie T., Ogura H., Hatano M. 1984a; Enhanced replication of human cytomegalovirus in human fibroblasts treated with dexamethasone. Journal of General Virology 65:1759–1767
     [Google Scholar]
  29. Tanaka J., Ogura T., Kamiya S., Yoshie T., Yabuki Y., Hatano M. 1984b; Dexamethasone enhances human cytomegalovirus replication in human epithelial cell cultures. Virology 136:448–451
     [Google Scholar]
  30. Tanaka J., Kamiya S., Ogura T., Sato H., Ogura H., Hatano M. 1985; Effect of dimethyl sulfoxide on interaction of human cytomegalovirus with host cell: conversion of nonproductive state of cell to a productive state for virus replication. Virology 146:165–176
     [Google Scholar]
  31. Tumilowicz J. J., Nicholes W. W., Cholon J. J., Greene A. E. 1970; Definition of continuous human cell line derived from neuroblastoma. Cancer Research 30:2110–2118
     [Google Scholar]
  32. Wathen M. W., Stinski M. F. 1982; Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early, and late times after infection. Journal of Virology 41:462–477
     [Google Scholar]
  33. Wathen M. W., Thomsen D. R., Stinski M. F. 1981; Temporal regulation of human cytomegalovirus transcription at immediate early and early times after infection. Journal of Virology 38:446–459
     [Google Scholar]
  34. Wroblewska W., Wellish M. C., Wolinsky J. S., Gilden D. 1981; Comparison of human cytomegalovirus growth in MRC-5 human fibroblasts, brain, and choroid plexus cells. Journal of Medical Virology 8:245–256
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-12-2605
Loading
Human Cytomegalovirus Persistent Infection in a Human Central Nervous System Cell Line: Production of a Variant Virus with Different Growth Characteristics
J. Gen. Virol. 67, 2605 (1986); https://doi.org/10.1099/0022-1317-67-12-2605
/content/journal/jgv/10.1099/0022-1317-67-12-2605
/content/journal/jgv/10.1099/0022-1317-67-12-2605
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error