RT Journal Article SR Electronic(1) A1 Jambou, Robert C. A1 Elango, Narayanasamy A1 Venkatesan, Sundararajan A1 Collins, Peter L.YR 1986 T1 Complete Sequence of the Major Nucleocapsid Protein Gene of Human Parainfluenza Type 3 Virus: Comparison with Other Negative Strand Viruses JF Journal of General Virology, VO 67 IS 11 SP 2543 OP 2548 DO https://doi.org/10.1099/0022-1317-67-11-2543 PB Microbiology Society, SN 1465-2099, AB Summary The sequence of the major nucleocapsid protein (NP) mRNA and its encoded protein were deduced by sequencing a cDNA clone representing the complete mRNA. The cDNA sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic RNA by primer extension using synthetic oligonucleotides. The NP mRNA contains 1641 nucleotides exclusive of poly(A) and encodes an NP protein of 515 amino acids. Alignment of the human parainfluenza type 3 virus (PF3) NP protein sequence with that of Sendai virus showed that the two proteins shared considerable sequence identity (58.8%). Additional comparisons provided highly significant statistical evidence that the PF3 NP protein sequence is related to those of measles and canine distemper viruses, but there was no evidence of relatedness with the nucleocapsid proteins of respiratory syncytial virus, influenza B virus, or vesicular stomatitis virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-11-2543