Shedding and Interspecies Type Sero-reactivity of the Envelope Glycopolypeptide gp120 of the Human Immunodeficiency Virus Free

Abstract

Summary

Two glycopolypeptides with molecular weights 160000 and 120000 (gp120) are regularly recognized by human immunodeficiency virus (HIV)-specific antisera in lysates of cells persistently infected with HIV. In the present study, gp120 was characterized as the major envelope glycopolypeptide of HIV. Gp120 was identified as the external viral glycoprotein by radiosequencing and by its presence in purified virus. However gp120 was predominantly shed as a soluble protein into the culture fluid. Furthermore gp120 was precipitated by sera from horses infected with equine infectious anaemia virus (EIAV), but not by sera from uninfected animals. This may indicate conserved epitopes common to the envelopes of HIV and EIAV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-11-2533
1986-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/11/JV0670112533.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-11-2533&mimeType=html&fmt=ahah

References

  1. Allan J. S., Coligan J. E., Barin F., Mclane M. F., Sodroski J. G., Rosen C. A., Haseltine W. A., Lee T. H., Essex M. 1985; Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III. Science 228:1091–1094
    [Google Scholar]
  2. Barre-Sinoussl F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vezinet-Brun F., Rouzioux C., Rozenbaum W., Montagnier L. 1983; Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 220:868–871
    [Google Scholar]
  3. Bolognesi D. P., Langlois A. J., Schafer W. 1975; Polypeptides of mammalian oncornaviruses. IV. Structural components of murine leukemia virus released as soluble antigens in cell culture. Virology 68:550–555
    [Google Scholar]
  4. Burny A., Bruck C., Chantrenne H., Cleuter Y., Dekegel D., Ghysdael J., Kettman R., Leclerque M. J., Leunen J., Mammerickx M., Portetelle D. 1980; Bovine leukemia virus: molecular biology and immunology. In Viral Oncology pp 231–289 Edited by Klein G. New York: Raven Press;
    [Google Scholar]
  5. Chen I. S. Y., Quan S. G., Golde D. W. 1983; Human T-cell leukemia virus type-II transforms normal human lymphocytes. Proceedings of the National Academy of Sciences, U,. S,. A 80:7006–7009
    [Google Scholar]
  6. Chiu I. M., Yaniv A., Dahlberg J. E., Gazit A., Skuntz S. F., Tronick S. R., Aaronson S. A. 1985; Nucleotide sequence evidence for relationship of AIDS retrovirus to lentiviruses. Nature, London 317:366–368
    [Google Scholar]
  7. Daniel M. D., Letvin N. L., King N. W., Kannagi M., Sehgal P. K., Hunt R. D. 1985; Isolation of T-cell tropic HTLV-III like retrovirus from macaques. Science 228:1201–1204
    [Google Scholar]
  8. Evatt B. L., Gomperts D. E., McDougal J. S., Ramsey R. B. 1985; Coincidental appearance of LAV/HTLV-III antibodies in hemophiliacs and the onset of the AIDS epidemic. New England Journal of Medicine 312:483–486
    [Google Scholar]
  9. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske, Safai B., White G., Foster P., Markham P. D. 1984; Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science 224:500–503
    [Google Scholar]
  10. Gelderblom H. R., Reupke H., Pauli G. 1985; Loss of envelope antigens of HTLV-III/LA V, a factor in AIDS pathogenesis?. Lancet ii:1016–1017
    [Google Scholar]
  11. Gonda M. A., Wong-Staal F., Gallo R. C., Clements J. E., Narayan O., Gilden R. V. 1985; Sequence homology and morphologic similarity of HTLV-III and visna virus, a pathogenic lenti virus. Science 221:173–177
    [Google Scholar]
  12. Hunsmann G., Moennig V., Schafer W. 1975; Properties of mouse leukemia viruses. IX. Active and passive immunization of mice against Friend leukemia with isolated viral gp71 glycoprotein and its corresponding antiserum. Virology 66:327–329
    [Google Scholar]
  13. Hunsmann G., Schneider J., Schulz A. 1981; Immunoprevention of Friend virus-induced erythroleukemia by vaccination with viral envelope glycoprotein complexes. Virology 113:602–612
    [Google Scholar]
  14. Hunsmann G., Pedersen N. C., Thielen G. H., Bayer H. 1983; Active immunization with feline leukemia virus envelope glycoprotein suppresses growth of virus-induced feline sarcoma. Medical Microbiology and Immunology 171:233–241
    [Google Scholar]
  15. Hunsmann G., Schneider J., Bayer H., Jurkiewicz E., Yamamoto N. 1985; Structural and epidemiological features of primate lymphotropic retroviruses. In Retroviruses in Human Leukemia Lymphoma pp 109–118 Edited by Miwa M., Sugano H., Sugimura T., Weiss R. A. Tokyo: Japan Scientific Society Press;
    [Google Scholar]
  16. Ihle J. N., Hanna M. G. Jr 1977; Natural immunity to endogenous oncornaviruses in mice. Contemporary Topics in Immunobiology 6:169–194
    [Google Scholar]
  17. Kanki P. J., Alroy J., Essex M. 1985; Isolation of T-lymphotropic retrovirus related to HTLV-III/LAV from wild caught African green monkeys. Science 230:951–954
    [Google Scholar]
  18. Levy J. A., Kramer S., Landis J., Shimabukuro J., Oshiro L. 1984; Isolation of lymphopathic retroviruses from San Francisco patients with AIDS. Science 225:840–842
    [Google Scholar]
  19. Lutley R., Petursson G., Palsson P. A., Georgsson G., Klein J., Nathanson N. 1983; Antigenic drift in visna: virus variation during long-term infection of Icelandic sheep. Journal of General Virology 64:1433–1440
    [Google Scholar]
  20. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki I., Shiraishi Y., Nagata K., Hinuma Y. 1981; Detection of type C viral particles in a cord T-cell line derived by cocultivation of normal human leukocytes and human leukemic T-cells. Nature, London 294:770–771
    [Google Scholar]
  21. Montagnier L., Dauguet C., Axler C., Chamaret S., Gruest J., Nugeyre M. T., Rey F., Barre-Sinoussi F., Chermann J. C. 1984; A new type of retrovirus isolated from patients presenting with lymphadenopathy and acquired immune deficiency syndrome: structural and antigenic relatedness with equine infectious anaemia virus. Annales de Virologie 135 E:119–134
    [Google Scholar]
  22. Montagnier L., Clavel F., Krust B., Chamaret S., Rey F., Barre-Sinoussi F., Chermann J. C. 1985; Identification and antigenicity of the major envelope glycoprotein of the lymphadenopathy-associated virus. Virology 144:283–298
    [Google Scholar]
  23. Montelaro R. C., Parekh B., Orrego A., Issel C. J. 1984; Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. Journal of Biological Chemistry 259:10539–10544
    [Google Scholar]
  24. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. 1984; Detection, isolation and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500
    [Google Scholar]
  25. Ratner L., Haseltine W., Patarca R., Livak K. J., Starcich B., Josephs S. F., Doran E. R., Rafalski J. A., Whitehorn E. A., Baumeister K., Ivanoff L., Petteway S. R. Jr, Pearson M. L., Lautenberger J. A., Papas T. S., Ghrayeb J., Chang N. T., Gallo R. C., Wong-Staal F. 1985; Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature, London 313:277–284
    [Google Scholar]
  26. Sanchez-Pescador R., Power M. D., Barr P. J., Steimer K. S., Stempien M. M., Brown-Shimer S. L., Gee W. W., Renard A., Randolph A., Levy J. A., Dina D., Luciw P. A. 1985; Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). Science 227:484–492
    [Google Scholar]
  27. Schneider J., Hunsmann G. 1978; Surface expression of murine leukemia virus structural polypeptides on host cells and the virion. International Journal of Cancer 22:204–213
    [Google Scholar]
  28. Schneider J., Yamamoto N., Hinuma Y., Hunsmann G. 1984; Sera from adult T-cell leukemia patients react with envelope and core polypeptides of adult T-cell leukemia virus. Virology 132:1–11
    [Google Scholar]
  29. Schneider J., Bayer H., Bienzle U., Hunsmann G. 1985; A glycopolypeptide (gp 100) is the main antigen detected by HTLV-III antisera. Medical Microbiology and Immunology 174:35–42
    [Google Scholar]
  30. Schneider J., Jurkiewicz E., Hayami M., Desrosiers R. C., Marx P., Hunsmann G. 1986; Serological and structural comparison of HIV, SIVmac, SIVagm, and SIVsm, four primate lentiviruses. Annales de l’Institut Pasteur (in press)
    [Google Scholar]
  31. Shih Y. T., Stokes P. E., Smythers G. W., Dhar R., Oroszlan S. 1982; Characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses. Journal of Biological Chemistry 257:11767–11773
    [Google Scholar]
  32. Thiel H. -J., Bergholz C., Beug H., Deinhardt F., Schwarz H., Schafer W. 1977; Isolation of the major glycoprotein (gp70) of simian sarcoma virus (SSV-l/SSAV-1) in preparative quantities. Zeitschrift für Naturforschung 32c:884–886
    [Google Scholar]
  33. Wain-Hobson S., Sonigo P., Danos O., Cole S., Alizon M. 1985; Nucleotide sequence of the AIDS virus, LAV. Cell 40:9–17
    [Google Scholar]
  34. Yamamoto N., Schneider J., Hinuma Y., Hunsmann G. 1982; Adult T-cell leukemia associated antigen (ATLA): detection of a glycoprotein in cell- and virus-free supernatant. Zeitschrift für Naturforschung 37C:731–732
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-11-2533
Loading
/content/journal/jgv/10.1099/0022-1317-67-11-2533
Loading

Data & Media loading...

Most cited Most Cited RSS feed