@article{mbs:/content/journal/jgv/10.1099/0022-1317-67-11-2497, author = "Shiraki, Kimiyasu and Rapp, Fred", title = "Establishment of Herpes Simplex Virus Latency in vitro with Cycloheximide", journal= "Journal of General Virology", year = "1986", volume = "67", number = "11", pages = "2497-2500", doi = "https://doi.org/10.1099/0022-1317-67-11-2497", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-11-2497", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "cycloheximide", keywords = "latency", keywords = "HSV", abstract = "Summary Human embryonic lung cells were infected with herpes simplex virus (HSV), treated with 10 µg/ml or more of cycloheximide for 24 h, incubated at 37 °C, and then shifted to 40.5 °C for various periods of time (0 to 40 days) without cycloheximide treatment. No infectious virus was detected after freezing and thawing of the cultures; however, infectious virus was recovered after temperature shift-down to 37 °C or superinfection with human cytomegalovirus (HCMV). The time course for formation of infectious centres after temperature shift-down was examined with and without HCMV superinfection during incubation at 40.5 °C. Two patterns of latently infected cells were identified: one pattern showed spontaneous reactivation of virus after temperature shift-down, and the second showed reactivation of HSV after superinfection with HCMV. The first pattern showed a rapid decrease in the number of infectious centres with time, whereas the second maintained a steady reactivation rate up to 40 days at 40.5 °C. The same tendency was observed for infectious centre formation at 37 °C with and without HCMV superinfection in the HSV latency system established with (E)-5-(2-bromovinyl)-2′-deoxyuridine and interferon treatment.", }