1887

Abstract

Summary

Berne virus possesses haemagglutinating activity which is inhibited by antisera that neutralize the infectivity of the virus. In decreasing order, human, rabbit and guinea-pig erythrocytes were agglutinated whereas agglutination was not observed with rat, goose, chicken or horse red blood cells. This pattern is different from that seen with the closely related Breda virus of cattle. Haemagglutinin was found to co-sediment with viral infectivity in sucrose density gradients. Transmission electron microscopy showed that intact virus particles form bridges between adjacent erythrocytes. The viral envelope was seen at a distance from the erythrocyte surface suggesting that the peplomers possess haemagglutinating activity. Haemagglutination was decreased in the presence of fetuin and gangliosides and also by pretreatment of the erythrocytes with periodate, suggesting that the virus binds to glycoproteins and/or glycolipids on the erythrocyte surface.

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/content/journal/jgv/10.1099/0022-1317-67-11-2485
1986-11-01
2024-10-14
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References

  1. Armstrong G. D., Paul R. W., Lee P. W. K. 1984; Studies on reovirus receptors of L cells: virus binding characteristics and comparison with reovirus receptors of erythrocytes. Virology 138:37–48
    [Google Scholar]
  2. Bächi T., Deas I. E., Howe C. 1977; Virus-erythrocyte membrane interactions. In Virus Infection and the Cell Surface pp 83–127 Edited by Poste G., Nicolson G. L. Amsterdam, New York & Oxford: North-Holland;
    [Google Scholar]
  3. Horzinek M. C., Weiss M. 1984; Toroviridae: a taxonomic proposal. Zentralblatt für Veterinärmedizin B 31:649–659
    [Google Scholar]
  4. Horzinek M. C., Weiss M., Ederveen J. 1984; Berne virus is not ‘coronavirus-like’. Journal of General Virology 65:645–649
    [Google Scholar]
  5. Horzinek M. C., Ederveen J., Weiss M. 1985; The nucleocapsid of Berne virus. Journal of General Virology 66:1287–1296
    [Google Scholar]
  6. Horzinek M. C., Ederveen J., Kaeffer B., De Boer D., Weiss M. 1986; The peplomers of Berne virus. Journal of General Virology 67:2475–2483
    [Google Scholar]
  7. Koopmans M., Ederveen J., Woode G. N., Horzinek M. C. 1986; The surface proteins of Breda virus. American Journal of Veterinary Research (in press)
    [Google Scholar]
  8. Weiss M., Steck F., Horzinek M. C. 1983; Purification and partial characterization of a new enveloped RNA virus (Berne virus). Journal of General Virology 64:1849–1858
    [Google Scholar]
  9. Weiss M., Steck F., Kaderli R., Horzinek M. C. 1984; Antibodies to Berne virus in horses and other animals. Veterinary Microbiology 9:523–531
    [Google Scholar]
  10. Woode G. N., Reed D. E., Runnels P. M., Herrig M. A., Hill H. T. 1982; Studies with an unclassified virus isolated from diarrheic calves. Veterinary Microbiology 7:221–240
    [Google Scholar]
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