1887

Abstract

Summary

Five neutralizing monoclonal antibodies produced against human rotavirus (HRV) serotypes 1, 2, 3 and 4 and the simian rotavirus (SA11) were used to study 59 rotavirus isolates of human, simian and feline origin previously serotyped using polyclonal antisera. In neutralization tests, 19 of 26 HRV serotype 1 isolates, both strains of HRV serotype 2, 14 of 24 HRV serotype 3 isolates and all of seven serotype 4 isolates were neutralized by the homologous serotype-specific monoclonal antibodies. Use of the panel of monoclonal antibodies revealed antigenic differences between strains within serotypes 1 and 3 and, in the case of the serotype 3 strains, each variant had a unique RNA electropherotype. An enzyme immunoassay (EIA) which utilized the monoclonal antibodies essentially confirmed the neutralization results. Preliminary results show that direct serotyping in faecal extracts by EIA using these monoclonal antibodies is specific but lacks sensitivity.

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1986-11-01
2022-07-02
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References

  1. Beards G. M., Flewett T. H. 1984; Serological characterisation of human rotaviruses propagated in cell cultures. Archives of Virology 80:231–237
    [Google Scholar]
  2. Birch C. J., Rodger S. M., Marshall J. A., Gust I. D. 1983; Replication of human rotavirus in cell culture. Journal of Medical Virology 11:241–250
    [Google Scholar]
  3. Birch C. J., Heath R. L., Marshall J. A., Liu S., Gust I. D. 1985; Isolation of feline rotaviruses and their relationship to human and simian isolates by electropherotype and serotype. Journal of General Virology 66:2731–2735
    [Google Scholar]
  4. Coulson B. S., Fowler K. J., Bishop R. F., Cotton R. G. H. 1985; Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates. Journal of Virology 54:14–20
    [Google Scholar]
  5. Crainic R., Couillin P., Blondel B., Cabau N., Boue A., Horodniceanu F. 1983; Natural variation of poliovirus neutralization epitopes. Infection and Immunity 41:1217–1225
    [Google Scholar]
  6. Ericson B. L., Graham D. Y., Mason B. B., Estes M. K. 1982; Identification, synthesis and modifications of simian rotavirus SA11 polypeptides in infected cells. Journal of Virology 42:825–839
    [Google Scholar]
  7. Fonteyne J., Zissis G., Lambert J. P. 1978; Recurrent rotavirus gastroenteritis. Lancet i:983
    [Google Scholar]
  8. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  9. Gerna G., Battaglia M., Milenesi G., Passarani N., Percivalle E., Cattaneo E. 1984; Serotyping of Cell culture-adapted subgroup 2 human rotavirus strains by neutralization. Infection and Immunity 43:722–729
    [Google Scholar]
  10. Greenberg H. B., Flores I., Kalica A. R., Wyatt R. G., Jones R. 1983; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  11. Gust I. D., Pringle R. C., Barnes G. L., Davidson G. P., Bishop R. F. 1977; Complement fixing antibody response to rotavirus infection. Journal of Clinical Microbiology 5:125–130
    [Google Scholar]
  12. Hoshino Y., Wyatt R. G., Flores J., Midthun K., Kapikian A. Z. 1985a; Serotypic characterization of rotaviruses derived from asymptomatic neonatal infections. Journal of Clinical Microbiology 21:425–430
    [Google Scholar]
  13. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. 1985b; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A. 82:8701–8704
    [Google Scholar]
  14. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981; Genes of the human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  15. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D., Flores J., Chanock R. M. 1981; Antigenic characterisation of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infection and Immunity 33:415–425
    [Google Scholar]
  16. Kapikian A. Z., Wyatt R. G., Levine M. M., Yolken R. H., Vankirk D. H., Dolin R., Greenberg H. B., Chanock R. M. 1983; Oral administration of human rotavirus to volunteers: induction of illness and correlates of resistance. Journal of Infectious Diseases 147:95–106
    [Google Scholar]
  17. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  18. Lambert J. P., Marbehant P., Marissens D., Zissis G. 1984; Monoclonal antibodies directed against different antigenic determinants of rotavirus. Journal of Virology 51:47–51
    [Google Scholar]
  19. Lee P. W., Hayes E. C., Joklik W. K. 1981; Characterisation of anti-reovirus immunoglobulins secreted by cloned hybridoma cell lines. Virology 108:134–146
    [Google Scholar]
  20. Liu S., Birch C. J., Coulepis A., Gust I. D. 1984; Radioimmunofocus assay for detection and quantitation of human rotavirus. Journal of Clinical Microbiology 20:347–350
    [Google Scholar]
  21. Mason B. B., Graham D. Y., Estes M. K. 1983; Biochemical mapping of the simian rotavirus SA11 genome. Journal of Virology 46:413–423
    [Google Scholar]
  22. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. 1985; A candidate for a new serotype of human rotavirus. Journal of Virology 54:623–624
    [Google Scholar]
  23. Merril C. R., Goldman D., Van Keuren M. L. 1982; Simplified silver protein detection and image enhancement methods in polyacrylamide gels. Electrophoresis 3:17–23
    [Google Scholar]
  24. Offit P. A., Blavat G. 1986; Identification of the two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  25. Rodger S. M., Bishop R. F., Birch C., Mclean B., Holmes I. H. 1981; Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  26. Rodriguez W. J., Kim H. W., Brandt C. D., Yolken R. H., Arrobio J. O., Kapikian A. Z., Chanock R. M., Parrott R. H. 1978; Sequential enteric illness associated with different rotavirus serotypes. Lancet ii:37
    [Google Scholar]
  27. Sammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver-based colour staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135–141
    [Google Scholar]
  28. Sato K., Inaba Y., Shinogaki T., Fujii R., Matsumoto M. 1981; Isolation of human rotavirus in cell culture. Archives of Virology 69:155–160
    [Google Scholar]
  29. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. 1985; Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. Journal of Clinical Microbiology 22:286–291
    [Google Scholar]
  30. Taniguchi K., Urasawa S., Urasawa T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  31. Urasawa T., Urasawa S., Taniguchi T. 1981; Sequential passage of human rotavirus in MA-104 cells. Microbiology and Immunology 25:1025–1035
    [Google Scholar]
  32. Vesikari T., Isolauri E., D’Hondt E., Delem A., Andre F. E., Zissis G. 1984; Protection of infants against rotavirus diarrhoea by RIT-4237 attenuated bovine rotavirus strain vaccine. Lancet i:977–981
    [Google Scholar]
  33. Webster R. G., Brown L. E., Jackson D. C. 1983; Changes in the antigenicity of the hemagglutinin molecule of H3 influenza virus at acidic pH. Virology 126:587–599
    [Google Scholar]
  34. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. S. 1982; Definition of human rotavirus serotypes by plaque reduction assay. Infection and Immunity 37:110–115
    [Google Scholar]
  35. Wyatt R. G., James H. D-., Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983; Direct isolation in cell culture of human rotaviruses and their characterisation into four serotypes. Journal of Clinical Microbiology 18:310–317
    [Google Scholar]
  36. Yolken R. H., Wyatt R. G., Zissis G., Brandt C. D., Rodriguez W. J., Kim H. W., Parrott R. H., Uruttia J. J., Mata L., Greenberg H. B., Kapikian A. Z., Chanock R. M. 1978; Epidemiology of human rotavirus types 1 and 2 as studied by enzyme-linked immunosorbent assay. New England Journal of Medicine 299:1156–1161
    [Google Scholar]
  37. Zissis G., Lambert J. P. 1978; Different serotypes of human rotaviruses. Lancet i:38–40
    [Google Scholar]
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