1887

Abstract

Summary

The interaction between the flavivirus West Nile virus (WNV) and cells of the mouse macrophage-like cell line, P388D1, was assayed by transmission electron microscopy, by following the association of [S]methionine-labelled virus with cells, and by using a radiobinding assay with an I-labelled F(ab′) fragment of a monoclonal antibody directed against the major viral envelope surface glycoprotein. Using electron microscopy, both fusion and endocytosis were observed at pH 6.4, but at pH 8.0 only endocytosis was observed. When S-labelled WNV was bound to the P388D1 cell surface at 0 °C, less virus eluted on warming to 37 °C at mildly acidic than at alkaline or neutral pH values. The monoclonal antibody fragment had an increased affinity for cell surface viral E glycoprotein after prebound WNV was warmed at mildly acidic pH values. It is proposed that the warming of cell-virus mixtures at low pH results in fusion with a consequent reduction in elution of virus and an increase in the recognition of cell surface-expressed viral envelope glycoprotein by labelled antibody.

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1986-11-01
2021-10-20
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References

  1. Cammack N., Gould E. A. 1985; Conditions for haemolysis by flaviviruses and characterization of the haemolysin. Journal of General Virology 66:2291–2296
    [Google Scholar]
  2. Choppin P. W., Compans R. W. 1975; Replication of paramyxoviruses. In Comprehensive Virology vol 4 pp 95–178 Edited by H. Fraenkel-Conrat, Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  3. Dales S. 1973; Early events in cell-animal virus interactions. Bacteriological Reviews 37:103–135
    [Google Scholar]
  4. Edwards J., Mann E., Brown D. T. 1983; Conformational changes in Sindbis virus envelope proteins accompanying exposure to low pH. Journal of Virology 45:1090–1097
    [Google Scholar]
  5. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgGl, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  6. Gollins S. W., Porterfield J. S. 1984; Flavivirus infection enhancement in macrophages: radioactive and biological studies on the effect of antibody on viral fate. Journal of General Virology 65:1261–1272
    [Google Scholar]
  7. Gollins S. W., Porterfield J. S. 1985; Flavivirus infection enhancement in macrophages: an electron microscopic study of viral cellular entry. Journal of General Virology 66:1969–1982
    [Google Scholar]
  8. Gollins S. W., Porterfield J. S. 1986a; pH-dependent fusion between the flavivirus West Nile and liposomal model membranes. Journal of General Virology 67:157–166
    [Google Scholar]
  9. Gollins S. W., Porterfield J. S. 1986b; The uncoating and infectivity of the flavivirus West Nile on interaction with cells: effects of pH and ammonium chloride. Journal of General Virology 67:1941–1950
    [Google Scholar]
  10. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, I-ondon 194:495–496
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Jjondon 227:680–685
    [Google Scholar]
  12. Longberg-Holm K., Philipson L. 1974; Early interactions between animal viruses and cells. In Monographs in Virology vol 9 pp 1–149 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  13. Madrid A. T. de, Porterfield J. S. 1969; A simple micro-culture method for the study of group B arboviruses. Bulletin of the World Health Organization 40:113–121
    [Google Scholar]
  14. Marsh M. 1984; The entry of enveloped viruses into cells by endocytosis. Biochemical Journal 218:1–10
    [Google Scholar]
  15. Mason D. W., Williams A. F. 1980; The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochemical Journal 187:1–20
    [Google Scholar]
  16. Matlin K. S., Reggio H., Helenius A., Simons K. 1981; Infectious entry pathway of influenza virus in a canine kidney cell line. Journal of Cell Biology 91:601–613
    [Google Scholar]
  17. Matlin K. S., Reggio H., Helenius A., Simons K. 1982; Pathway of vesicular stomatitis virus entry leading to infection. Journal of Molecular Biology 156:609–631
    [Google Scholar]
  18. Nestorowicz A., Laver G., Jackson D. C. 1985; Antigenic determinants of influenza virus haemagglutinin. X. A comparison of the physical and antigenic properties of monomeric and trimeric forms. Journal of General Virology 66:1687–1695
    [Google Scholar]
  19. Parham P. 1983; On the fragmentation of monoclonal IgGl, IgG2a, and IgG2b from Balb/c mice. Journal of Immunology 131:2895–2902
    [Google Scholar]
  20. Peiris J. S. M., Porterfield J. S., Roehrig J. T. 1982; Monoclonal antibodies against the flavivirus West Nile. Journal of General Virology 58:283–289
    [Google Scholar]
  21. Porterfield J. S., Rowe C. E. 1960; Haemagglutination with arthropod-borne viruses and its inhibition by certain phospholipids. Virology 11:765–770
    [Google Scholar]
  22. Poste G., Pasternak C. A. 1978; Virus-induced cell fusion. In Cell Surface Reviews vol 5 pp 305–367 Edited by Poste G., Nicolson G. L. Amsterdam:: Elsevier/North-Holland;
    [Google Scholar]
  23. Vaananen P., Kaariainen L. 1980; Fusion and haemolysis of erythrocytes caused by three togaviruses: Semliki Forest, Sindbis and rubella. Journal of General Virology 46:467–475
    [Google Scholar]
  24. White J., Kartenbeck J., Helenius A. 1980; Fusion of Semliki Forest virus with the plasma membrane can be induced by low pH. Journal of Cell Biology 87:264–272
    [Google Scholar]
  25. White J., Kielian M., Helenius A. 1983; Membrane fusion proteins of enveloped animal viruses. Quarterly Reviews of Biophysics 16:151–195
    [Google Scholar]
  26. Yewdell J. W., Gerhard W., Bachi T. 1983; Monoclonal anti-hemagglutinin antibodies detect irreversible antigenic alterations that coincide with the acid activation of influenza virus A/PR/834-mediated hemolysis. Journal of Virology 48:239–248
    [Google Scholar]
  27. Yoshimura A., Kuroda K., Kawasaki K., Yamashina S., Maeda T., Ohnishi S. 1982; Infectious cell entry mechanism of influenza virus. Journal of Virology 43:284–293
    [Google Scholar]
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