1887

Abstract

Summary

Non-capsid poliovirus proteins of the P2 region in extracts of infected cells were solubilized by SDS, separated by PAGE, electroeluted from the gels and used to immunize mice. The sera obtained were rendered monospecific by extensive absorption with proteins from uninfected cell extracts, and the spleen cells of these animals were used to establish antibody-secreting hybridomas. The monoclonal and the monospecific antibodies recognized denatured antigen (proteins 2C, 2BC and P2) in ELISA, immunoblot and immunoprecipitation tests and also combined with the native proteins of the P2 region in immunoprecipitation. In addition, the antibodies could be used successfully for immunofluorescence and electron microscopic immunocytochemistry.

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1986-11-01
2022-06-27
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References

  1. Altman L. G., Schneider B. G., Papermaster D. S. 1984; Rapid embedding of tissue in Lowicryl K4M for immunoelectron microscopy. Journal of Histochemistry and Cytochemistry 32:1217–1223
    [Google Scholar]
  2. Armbruster B. L., Carlemalm E., Chiovetti R., Garavito R. M., Hobot J. A., Kellenberger E., Villiger W. 1982; Specimen preparation for electron microscopy using low temperature embedding resins. Journal of Microscopy 126:77–85
    [Google Scholar]
  3. Baron M. H., Baltimore D. 1982; Antibodies against a synthetic peptide of the poliovirus replicase protein: reaction with native, virus-encoded proteins and inhibition of virus-specific polymerase activities in vitro. Journal of Virology 43:969–978
    [Google Scholar]
  4. Bienz K., Egger D., Wolff D. A. 1973; Virus replication, cytopathology and lysosomal enzyme response of mitotic and interphase HEp-2 cells infected with poliovirus. Journal of Virology 11:565–574
    [Google Scholar]
  5. Bienz K., Egger D., Rasser Y., Bossart W. 1980; Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology 100:390–399
    [Google Scholar]
  6. Bienz K., Egger D., Easser Y., Bossart W. 1983; Intracellular distribution of poliovirus proteins and the induction of virus-specific cytoplasmic structures. Virology 131:39–48
    [Google Scholar]
  7. Bienz K., Egger D., Pasamontes L. 1986; Electron microscopic immunocytochemistry. Silver enhancement of colloidal gold marker allows double labeling with the same primary antibody. Journal of Histochemistry and Cytochemistry 34: (in press)
    [Google Scholar]
  8. Blondel B., Crainic R., Akacem O., Bruneau P., Girard M., Horodniceanu F. 1982; Evidence for common intertypic antigen determinants on poliovirus capsid polypeptides. Virology 123:461–463
    [Google Scholar]
  9. Carlemalm E., Garavito R.M., Villiger W. 1982; Resin development for electron microscopy and an analysis of embedding at low temperature. Journal of Microscopy 126:123–143
    [Google Scholar]
  10. Chow M., Baltimore D. 1982; Isolated poliovirus capsid protein VP1 induces a neutralizing response in rats. Proceedings of the National Academy of Sciences, U.S.A. 79:7518–7521
    [Google Scholar]
  11. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. 1982; Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. Journal of Virology 43:997–1005
    [Google Scholar]
  12. Emini E. A., Jameson B. A., Wimmer E. 1983; Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature, Jjjndon 304:699–703
    [Google Scholar]
  13. Emini E. A., Schleif W. A., Colonno R. J., Wimmer E. 1985; Antigenic conservation and divergence between the viral-specific proteins of poliovirus type 1 and various picornaviruses. Virology 140:13–20
    [Google Scholar]
  14. Ferguson M., Minor P. D., Magrath D. I., Qui Y. H., Spitz M., Schild G.C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  15. Flanegan J. B., Van Dyke T. A. 1979; Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro. Journal of Virology 32:155–161
    [Google Scholar]
  16. Hanecak R., Semler B. L., Anderson C.W., Wimmer E. 1982; Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage of glutamine-glycine pairs. Proceedings of the National Academy of Sciences, U.S.A. 79:3973–3977
    [Google Scholar]
  17. Hughes J. V., Stanton L. W. 1985; Isolation and immunizations with hepatitis A viral structural proteins: induction of antiprotein, antiviral, and neutralizing responses. Journal of Virology 55:395–401
    [Google Scholar]
  18. Koch F., Koch G. 1985 The Molecular Biology of Poliovirus Wien: Springer-Verlag;
    [Google Scholar]
  19. Kohler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  21. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  22. McLean I. W., Nakane P. K. 1974; Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. Journal of Histochemistry and Cytochemistry 22:1077–1083
    [Google Scholar]
  23. Mertens T., Pika U., Eggers H. J. 1983; Cross antigenicity among enteroviruses as revealed by immunoblot technique. Virology 129:431–442
    [Google Scholar]
  24. Nuss D. L., Oppermann H., Koch G. 1975; Selective blockage of initiation of host protein synthesis in RNA-virus-infected cells. Proceedings of the National Academy of Sciences, U.S.A. 72:1258–262
    [Google Scholar]
  25. Otto M., Snejdarkova M. 1981; A simple and rapid method for the quantitative isolation of proteins from polyacrylamide gels. Analytical Biochemistry 111:111–114
    [Google Scholar]
  26. Rueckert R. R., Wimmer E. 1984; Systematic nomenclature of picomavirus proteins. Journal of Virology 50:957–959
    [Google Scholar]
  27. Schneider C., Newman R. A., Sutherland D. R., Asser U., Greaves M. F. 1982; A One-Step purification of membrane proteins using a high efficiency immunomatrix. Journal of Biological Chemistry 257:10766–10769
    [Google Scholar]
  28. Semler B. L., Anderson C. W., Hanecak R., Dorner L. F., Wimmer E. 1982; A membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptide. Cell 28:405–412
    [Google Scholar]
  29. Semler B. L., Hanecak R., Dorner L. F., Anderson C. W., Wimmer E. 1983; Poliovirus RNA synthesis in vitro: structural elements and antibody inhibition. Virology 126:624–635
    [Google Scholar]
  30. Shulman M., Wilde C. D., Kohler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature, Ijtndon 276:269–270
    [Google Scholar]
  31. Tershak D. R. 1984; Association of poliovirus proteins with the endoplasmic reticulum. Journal of Virology 52:777–783
    [Google Scholar]
  32. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  33. Valnes K., Brandtzaeg P. 1985; Retardation of immunofluorescence fading during microscopy. Journal of Histochemistry and Cytochemistry 33:755–761
    [Google Scholar]
  34. Vrijsen R., Rombaut B., Boeye A. 1984; Intertypic cross-reaction of nonneutralizing monoclonal poliovirus antibodies. Journal of Virology 49:1002–1004
    [Google Scholar]
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