1887

Abstract

Summary

The organization of proviruses derived from infecting transforming viruses rescued from hamster tumour cells was studied. Southern blot analysis indicated that the provirus from the F6 cell line was organized as long terminal repeat (LTR)--LTR, and S1 mapping experiments suggested that it was probably derived by reverse transcription of mRNA followed by integration. In the E6 cell line, the provirus unit was arranged as LTR-Δ -LTR, indicating a recombination event between the rescued transforming virus and the helper virus. These results suggest that transforming defective viruses containing only the gene can be rescued from non-permissive mammalian cells.

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1986-11-01
2024-12-07
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References

  1. Adkins B., Hunter T. 1981; Identification of a packaged cellular mRNA in virions of Rous sarcoma virus. Journal of Virology 39:471–480
    [Google Scholar]
  2. Boettiger D. 1974; Reversion and induction of Rous sarcoma virus expression in virus-transformed baby hamster kidney cells. Virology 62:522–529
    [Google Scholar]
  3. Geryk J., Sainerova H., Sovova V., Svoboda J. 1984; Characterization of cryptovirogenic, virus-productive and helper-dependent virogenic hamster tumour cell lines. Folia biologica 30:152–154
    [Google Scholar]
  4. Guntaka R. V., Prasad R. Y., Mitsialis S. A., Katz R. A. 1980; Modification of avian sarcoma proviral DNA sequences in nonpermissive XC cells but not in permissive chick cells. Journal of Virology 34:569–572
    [Google Scholar]
  5. Hajkova V., Kristek J., Geryk J., Svoboda J. 1982; Characterization of a mutant of the Prague strain of Rous sarcoma virus inducing fusiform transformation of avian fibroblasts in vitro. Folia biologica 28:160–176
    [Google Scholar]
  6. Hughes S. H., Shank P. R., Spector D. H., Kung H. J., Bishop J. M., Varmus H. E., Vogt P. K., Breitman M. L. 1978; Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites. Cell 15:1397–1410
    [Google Scholar]
  7. Katz R. A., Omer C. A., Weis J. H., Mitsialis S. A., Faras A. J., Guntaka R. V. 1982; Restriction endonuclease and nucleotide sequence analyses of molecularly cloned unintegrated avian tumor virus DNA: structure of large terminal repeats in circle junctions. Journal of Virology 42:346–351
    [Google Scholar]
  8. Martin G. S., Radke K., Hughes S., Quintrell N., Bishop J. M., Varmus H. E. 1979; Mutants of Rous sarcoma virus with extensive deletions of the viral genome. Virology 96:530–546
    [Google Scholar]
  9. Martin G. S., Lee W. H., Duesberg P. H. 1980; Generation of nondefective Rous sarcoma virus by asymmetric recombination between deletion mutants. Journal of Virology 36:591–594
    [Google Scholar]
  10. Mason W. S., Hsu T. W., Yeater C., Sabran J. L., Mark G. E., Kaji A., Taylor J. M. 1979; Avian sarcoma virus-transformed quail clones defective in the production of focus-forming virus. Journal of Virology 30:132–140
    [Google Scholar]
  11. Mitsialis S. A., Caplan S., Guntaka R. V. 1983; An upstream regulatory domain of avian tumor virus long terminal repeat is required for expression of a procaryotic neomycin gene in eukaryotic cells. Molecular and Cellular Biology 3:1975–1984
    [Google Scholar]
  12. Murphy H. M. 1977; A new replication-defective variant of the Bryan high-titer strain of Rous sarcoma virus. Virology 77:705–721
    [Google Scholar]
  13. Popovic M., Svoboda J., Suni J., Vaheri A., Ponten J. 1977; Expression of viral protein p27 in avian sarcoma virus transformed mammalian cells and helper-dependent rescue of Rous sarcoma virus. International Journal of Cancer 19:834–842
    [Google Scholar]
  14. Pugatsch T., Stacey D. W. 1983; Identification of a sequence likely to be required for avian retroviral packaging. Virology 128:505–511
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Schwartz D. E., Tizard R., Gilbert W. 1983; Nucleotide sequence of Rous sarcoma virus. Cell 32:853–860
    [Google Scholar]
  17. Stacey D. W. 1980; Expression of a subgenomic retroviral messenger RNA. Cell 21:811–820
    [Google Scholar]
  18. Steimer K. S., Boettiger D. 1977; Complementation rescue of Rous sarcoma virus from transformed mammalian cells by polyethylene glycol-mediated cell fusion. Journal of Virology 23:133–141
    [Google Scholar]
  19. Svoboda J. 1981; Helper virus-dependent RSV rescue from two lines of hamster cells transformed in vivo by XC RSV. International Journal of Cancer 27:857–864
    [Google Scholar]
  20. Svoboda J., Dourmashkin R. 1969; Rescue of Rous sarcoma virus from virogenic mammalian cells associated with chicken cells and treated with Sendai virus. Journal of General Virology 4:523–529
    [Google Scholar]
  21. Svoboda J., Hlozanek T. 1970; Role of cell association in virus infection and virus rescue. Advances in Cancer Research 13:217–269
    [Google Scholar]
  22. Svoboda J., Hlozanek T., Machala O. 1968; Rescue of Rous sarcoma virus in mixed cultures of virogenic mammalian and chicken cells, treated and untreated with Sendai virus and detected by focus assay. Journal of General Virology 2:461–464
    [Google Scholar]
  23. Svoboda J., Machala O., Donner L., Sovova V. 1971; Comparative study of RSV rescue from RSV-transformed mammalian cells. International Journal of Cancer 8:391–400
    [Google Scholar]
  24. Svoboda J., Lhotak V., Geryk J., Saule S., Raes M. B., Stehelin D. 1983; Characterization of exogenous proviral sequences in hamster tumour cell lines transformed by Rous sarcoma virus rescued from XC cells. Virology 128:195–209
    [Google Scholar]
  25. Wang L. H., Stacey D. W. 1982; Participation of subgenomic retroviral mRNAs in recombination. Journal of Virology 41:919–930
    [Google Scholar]
  26. Wang L. H., Beckson M., Anderson S. M., Hanafusa H. 1984; Identification of the viral sequence required for the generation of recovered avian sarcoma viruses and characterization of a series of replication-defective recovered avian sarcoma viruses. Journal of Virology 49:881–891
    [Google Scholar]
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