1887

Abstract

Summary

Protoplasts derived from shoot cultures of potato cv. Cara, which carries immunity gene , supported only limited virus multiplication after inoculation with particles or RNA of isolate DX, a group 3 strain of potato virus X, as compared to similarly inoculated protoplasts of cultivars King Edward and Pentland Ivory which lack . The Cara protoplasts were, however, able to support extensive replication of the resistance-breaking strain HB. This strain-specific resistance did not appear to be mediated by a failure or inhibition of the uncoating mechanism or of virus assembly.

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1986-11-01
2021-10-25
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References

  1. Adams S. E., Jones R. A. C., Coutts R. H. A. 1985; Infection of protoplasts derived from potato shoot cultures with potato virus X. Journal of General Virology 66:1341–1346
    [Google Scholar]
  2. Barker H., Harrison B. D. 1984; Expression of genes for resistance to potato virus Y in potato plants and protoplasts. Annals of Applied Biology 105:539–545
    [Google Scholar]
  3. Cockerham G. 1955; Strains of potato virus X. Proceedings of the Second Conference on Potato Virus Diseases, Lisse-Wageningen 1954 pp 89–92
    [Google Scholar]
  4. Cockerham G. 1970; Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348
    [Google Scholar]
  5. Coutts R. H. A., Wood K. R. 1977; Inoculation of leaf mesophyll protoplasts from a resistant and a susceptible cucumber cultivar with cucumber mosaic virus. FEMS Microbiology Letters 1:121–124
    [Google Scholar]
  6. De Blas A. L., Cherwinski H. M. 1983; Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies. Analytical Biochemistry 133:214–219
    [Google Scholar]
  7. Foxe M. J., Prakash J. 1986; Expression of resistance to potato virus X in isolated potato protoplasts. Archives of Virology 88:167–174
    [Google Scholar]
  8. Furusawa I., Okuno T. 1978; Infection with BMV of mesophyll protoplasts isolated from five plant species. Journal of General Virology 40:489–491
    [Google Scholar]
  9. Gonda T. J., Symons R. H. 1979; Cucumber mosaic virus replication in cowpea protoplasts: time course of virus, coat protein and RNA synthesis. Journal of General Virology 45:723–736
    [Google Scholar]
  10. Harrison B. D. 1980; Active resistance of plants to viruses. In Active Defense Mechanisms in Plants pp 193–211 Edited by Wood R. K. S. New York: Plenum Press;
    [Google Scholar]
  11. Huber R., Rezelman G., Hibi T., Van Kammen A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  12. Jones R. A. C. 1985; Further studies on resistance breaking strains of potato virus X. Plant Pathology 34:182–189
    [Google Scholar]
  13. Kiefer M. C., Bruening G., Russell M. L. 1984; RNA and capsid accumulation in cowpea protoplasts that are resistant to cowpea mosaic virus strain SB. Virology 137:371–381
    [Google Scholar]
  14. Koike M., Hibi T., Yora K. 1977; Infection of cowpea mesophyll protoplasts with cucumber mosaic virus. Virology 83:413–416
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Maule A. J., Boulton M. I., Wood K. R. 1980; Resistance of cucumber protoplasts to cucumber mosaic virus: a comparative study. Journal of General Virology 51:271–279
    [Google Scholar]
  17. Moreira A., Jones R. A. C., Fribourg C. E. 1980; Properties of a resistance-breaking strain of potato virus X. Annals of Applied Biology 95:93–103
    [Google Scholar]
  18. Motoyoshi F., OSHIMA N. 1977; Expression of genetically controlled resistance to tobacco mosaic virus infection in isolated tomato leaf mesophyll protoplasts. Journal of General Virology 34:499–506
    [Google Scholar]
  19. Motoyoshi F., Oshima N. 1979; Standardization in inoculation procedure and effect of a resistance gene on infection of tomato protoplasts with tobacco mosaic virus RNA. Journal of General Virology 44:801–806
    [Google Scholar]
  20. Nassuth A. 1982; Alfalfa mosaic virus RNA replication in protoplasts. Ph,. D. thesis, University of Leiden
    [Google Scholar]
  21. Oldfield S. J., Coutts R. H. A. 1980; Isolation and infection of cowpea primary leaf protoplasts with tobacco necrosis virus. In Tissue Culture Methods for Plant Pathologists pp 87–93 Edited by Ingram D. S., Helgeson J. P. Oxford: Blackwells;
    [Google Scholar]
  22. Otsuki Y., Shimomura T., Takebe I. 1972; Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding varieties. Virology 50:45–50
    [Google Scholar]
  23. Sanderson J. L., Bruening G., Russell M. L. 1985; Possible molecular basis of immunity of cowpeas to cowpea mosaic virus. In UCLA: Cellular and Molecular Biology of Plant Stress pp 401–412 Edited by Kosuge T., Ray J. L. New York: Alan R. Liss;
    [Google Scholar]
  24. Sulzinski M. A., Zaitlin M. 1982; Tobacco mosaic virus replication in resistant and susceptible plants: in some resistant species virus is confined to a small number of initially infected cells. Virology 121:12–19
    [Google Scholar]
  25. Torrance L., Larkins A. P., Butcher G. W. 1986; Characterization of monoclonal antibodies against potato virus X and comparison of serotypes with resistance groups. Journal of General Virology 67:57–67
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences U.S.A. 76:4350–4354
    [Google Scholar]
  27. Widholm J. M. 1972; Use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
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