1887

Abstract

Summary

We analysed the production of interferons (IFN)-alpha and -gamma during the generation of human influenza-virus specific cytotoxic T lymphocyte (CTL) responses using monoclonal antibodies in a specific radioimmunoassay. The results showed that the peripheral blood mononuclear cells (PBM) of all donors tested produced IFN-gamma and had influenza A virus-specific CTL activity after stimulation. The amount of IFN-gamma produced and the level of CTL activity were significantly correlated. The PBM of some donors also produced IFN-alpha. The level of IFN-gamma produced was low during the first few days and increased subsequently, but IFN-alpha, when it was detected, was produced on day 1. The kinetics of the increase in IFN-gamma correlated with the increase in CTL activity. We also observed an increased percentage of cells bearing interleukin-2 receptors, which may have been a response to the production of IFN-gamma. The T cells active in lysing influenza A virus-infected target cells and in producing IFN-gamma were determined after separating effector cells with monoclonal antibodies. The CTL effector cells were mainly in the T8 subset, but IFN-gamma-producing cells were found in both T4 and T8 subsets. These results suggest that influenza virus-specific T8 CTL produce IFN-gamma in response to virus, and that T4 cells which are not CTL effectors also produce IFN-gamma after restimulation with influenza A virus-infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-11-2325
1986-11-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/11/JV0670112325.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-11-2325&mimeType=html&fmt=ahah

References

  1. Andrew M. E., Churilla A. M., Malek T. R., Braciale V. L., Braciale T. J. 1985; Activation of virus specific CTL clones: antigen-dependent regulation of interleukin 2 receptor expression. Journal of Immunology 134:920–925
    [Google Scholar]
  2. Balkwill F. R., Griffin B. E., Band H. A., Beverley P. C. L. 1983; Immune human lymphocytes produce an acid-labile alpha-interferon. Journal of Experimental Medicine 57:1059–1063
    [Google Scholar]
  3. Becker S. 1985; Interferon-gamma accelerates immune proliferation via its effect on monocyte HLA-DR expression. Cellular Immunology 91:301–307
    [Google Scholar]
  4. Biddison W. D., Sharrow S. O., Shearer G. M. 1981; T cell subpopulations required for the human cytotoxic T lymphocyte response to influenza virus: evidence for T cell help. Journal of Immunology 127:487–191
    [Google Scholar]
  5. Burlington D. B., Djeu J. Y., Wells M. A., Kiley S. C., Quinnan G. V. Jr 1984; Large granular lymphocytes provide an accessory function in the in vitro development of influenza A virus-specific cytotoxic T cells. Journal of Immunology 132:3154–3158
    [Google Scholar]
  6. Chonmaitree T., Roberts N. J. Jr, Douglas R. G. JR, Hall C. B., Simons R. L. 1981; Interferon production by human mononuclear leukocytes: differences between respiratory syncytial virus and influenza viruses. Infection and Immunity 32:300–303
    [Google Scholar]
  7. Czarniecki C. W., Fennie C. W., Powers D. B., Estell D. A. 1984; Synergistic antiviral and antiproliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. Journal of Virology 49:490–496
    [Google Scholar]
  8. Depper J. M., Leonard W. I., Robb R. J., Waldmann T. A., Greene W. C. 1983; Blockade of the interleukin-2 receptor by anti-TAC antibody: inhibition of human lymphocyte activation. Journal of Immunology 131:690–696
    [Google Scholar]
  9. Djeu J. Y., Stocks N., Zoon K., Stanton G. L., Timonen T., Herberman R. B. 1982; Positive self regulation of cytotoxicity in human natural killer cells by production of interferon upon exposure to influenza and herpes viruses. Journal of Experimental Medicine 156:1222–1234
    [Google Scholar]
  10. Ennis F. A., Meager A. 1981; Immune interferon produced to high levels by antigenic stimulation of human lymphocytes with influenza virus. Journal of Experimental Medicine 154:1279–1289
    [Google Scholar]
  11. Exley T., Parti S., Barwick S., Meager A. 1984; A comparison of the neutralizing properties of monoclonal and polyclonal antibodies to human interferon alpha. Journal of General Virology 65:2277–2280
    [Google Scholar]
  12. Farrar W. L., Johnson H. M., Farrar J. J. 1981; Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2. Journal of Immunology 126:1120–1125
    [Google Scholar]
  13. Fleischer B. 1982; Cloned lines of human cytotoxic T lymphocytes with specificity for influenza virus. Journal of Immunology 129:1731–1735
    [Google Scholar]
  14. Fleischmann W. R. JR, Georgiades J. A., Osborne L. C., Johnson H. M. 1979; Potentiation of interferon activity by mixed preparation of fibroblast and immune interferon. Infection and Immunity 26:248–253
    [Google Scholar]
  15. Johnson H. M., Farrar W. L. 1983; The role of a gamma interferon-like lymphokine in the activation of T cells for expression of interleukin 2 receptors. Cellular Immunology 75:154–159
    [Google Scholar]
  16. Kaplan D. R., Griffith R., Braciale V. L., Braciale T. J. 1984; Influenza virus-specific human cytotoxic T cell clones: heterogeneity in antigenic specificity and restriction by class II MHC products. Cellular Immunology 88:193–206
    [Google Scholar]
  17. Kelley V. E., Fiers W., Strom T. B. 1984; Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines. Journal of Immunology 132:240–245
    [Google Scholar]
  18. Klein J. R., Raulet D. H., Pasternack M. S., Bevan M. J. 1982; Cytotoxic T lymphocytes produce immune interferon in response to antigen or mitogen. Journal of Experimental Medicine 155:1198–1203
    [Google Scholar]
  19. Le J., Prensky W., Yip Y. K., Chang Z., Hoffman T., Stevenson H. C., Balazs I., Sadlik J. R., Vilcek J. 1983; Activation of human monocyte cytotoxicity by natural and recombinant immune interferon. Journal of Immunology 131:2821–2826
    [Google Scholar]
  20. McMichael A. J., Askonas B. A. 1978; Influenza virus-specific cytotoxic T cells in man: induction and properties of the cytotoxic cell. European Journal of Immunology 8:705–711
    [Google Scholar]
  21. McMichael A. J., Gotch F. M., Hildreth J. E. K. 1982; Lysis of allogeneic human lymphocytes by nonspecifically activated T-like cells. European Journal of Immunology 12:1002–1005
    [Google Scholar]
  22. McMichael A. J., Gotch F. M., Noble G. R., Beare P. A. S. 1983; Cytotoxic T-cell immunity to influenza. New England Journal of Medicine 309:13–17
    [Google Scholar]
  23. Mannel D. N., Falk W. 1983; Interferon-gamma is required in activation of macrophages for tumor cytotoxicity. Cellular Immunology 79:396–402
    [Google Scholar]
  24. Martinez-Maza O., Andersson U., Andersson J., Britton S., Deley M. 1984; Spontaneous production of interferon-gamma in adult and newborn humans. Journal of Immunology 132:251–255
    [Google Scholar]
  25. Meager A., Parti S., Barwick A., Spragg J., O’hagan K. 1984; Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. Journal of Interferon Research 4:619–625
    [Google Scholar]
  26. Morris A. G., Lin Y-L., Askonas B. A. 1982; Immune interferon release when a cloned cytotoxic T-cell line meets its correct influenza-infected target cells. Nature, London 295:150–152
    [Google Scholar]
  27. Roberts N. J. JR, Douglas R. G. JR, Simons R. M., Diamond M. E. 1979; Virus-induced interferon production by human macrophages. Journal of Immunology 123:365–369
    [Google Scholar]
  28. Secher D. S. 1981; Immunoradiometric assay of human leukocyte interferon using monoclonal antibody. Nature, London 290:501–503
    [Google Scholar]
  29. Shaw S., Biddison W. E. 1979; HLA-linked genetic control of the specificity of human cytotoxic T-cell responses to influenza virus. Journal of Experimental Medicine 149:565–575
    [Google Scholar]
  30. Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. 1982; Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. Journal of Experimental Medicine 156:1780–1793
    [Google Scholar]
  31. Trinchieri G., Perussia B. 1985; Immune interferon: a pleiotropic lymphokine with multiple effects. Immunology Today 6:131–136
    [Google Scholar]
  32. Wells M. A., Albrecht P., Ennis F. A. 1981a; Recovery from a viral respiratory infection. I. Influenza pneumonia in normal and T-deficient mice. Journal of Immunology 126:1036–1041
    [Google Scholar]
  33. Wells M. A., Ennis F. A., Albrecht P. 1981b; Recovery from a viral respiratory infection. II. Passive transfer of immune spleen cells to mice with influenza pneumonia. Journal of Immunology 126:1042–1046
    [Google Scholar]
  34. Wysocki L. J., Sato V. L. 1978; ‘Panning’ for lymphocytes: a method for cell selection. Proceedings of the National Academy of Sciences, U.S.A. 75:2844–2848
    [Google Scholar]
  35. Yap K. L., Ada G. L., McKenzie I. F. C. 1978; Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus. Nature London 273:238–23
    [Google Scholar]
  36. Zerial A., Hovanessian A. G., Stefanos S., Huygen K., Werner G. H., Falcoff E. 1982; Synergistic activities of type I (alpha, beta) and type II (gamma) murine interferons. Antiviral Research 2:227–239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-11-2325
Loading
/content/journal/jgv/10.1099/0022-1317-67-11-2325
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error