Acyclovir Efficiently Inhibits Oropharyngeal Excretion of Epstein-Barr Virus in Patients with Acute Infectious Mononucleosis Free

Abstract

Summary

Shedding of Epstein-Barr virus (EBV) into saliva was studied in 31 patients with verified acute infectious mononucleosis. The patients had been randomized for intravenous treatment with acyclovir (ACV) at 10 mg/kg body weight at 8 h intervals for 7 days, or placebo, in a double-blind trial. EBV in centrifuged throat washings was detected by transformation of umbilical cord lymphocytes and by immunofluorescence staining for EBV-associated nuclear antigen in fixed cell smears. Saliva samples were obtained before and during treatment, and after 4 weeks and 6 months, respectively. ACV effectively but transiently interrupted EBV production ( < 0.001), but virus shedding resumed at the initial level within 3 weeks of cessation of the treatment. Initially, 93.5% of the patients had detectable EBV in the saliva compared with 83% in the 4th week and 58% after 6 months.

Keyword(s): acyclovir , EBV and transformation
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1986-10-01
2024-03-29
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References

  1. Andersson J., Britton S., Ernberg I., Andersson U., Henle W., Sköldenberg B., Tisell A. 1986; Effect of acyclovir on infectious mononucleosis. A double blind, placebo-controlled study. Journal of Infectious Diseases 153:283–290
    [Google Scholar]
  2. Biron K. K., Elion G. B. 1980; In vitro susceptibility of varicella-zoster virus to acyclovir. Antimicrobial Agents and Chemotherapy 18:443–447
    [Google Scholar]
  3. Chang R., Golden H. 1971; Transformation of human leukocytes by washings from infectious mononucleosis patients. Nature, London 234:359–360
    [Google Scholar]
  4. Colby B., Shaw J., Elion G. B., Pagano J. 1979; Effect of acyclovir on Epstein-Barr virus DNA replication. Journal of Virology 34:560–568
    [Google Scholar]
  5. Colby B., Shaw J., Datta A., Pagano J. 1982; Replication of Epstein-Barr virus DNA in lymphoblastoid cells treated for extended periods with acyclovir. American Journal of Medicine 73:77–81
    [Google Scholar]
  6. Datta A. K., Colby B. M., Shaw I. E., Pagano J. S. 1980; Acyclovir inhibition of Epstein-Barr virus replication. Proceedings of the National Academy of Sciences, U.S.A 77:5163–5166
    [Google Scholar]
  7. De Miranda P., Blum M. R. 1983; Pharmakokinetics of acyclovir after intravenous and oral administration. Journal of Antimicrobial Chemotherapy 12: supplement B 29–37
    [Google Scholar]
  8. Elion G., Furman P., Fyfe J., De Miranda P., Beauchamp L., Schaffer H. 1977; Selectivity of action of an antiherpetic agent, 9-(2-hydroxy ethoxymethyl)guanine. Proceedings of the National Academy of Sciences, U.S.A. 74:5716–5720
    [Google Scholar]
  9. Furman P. A., St Clair M. H., Fyfe J. A., Rideout J. L., Keller P. M., Elion G. B. 1979; Inhibition of herpes simplex virus induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxy ethoxymethyl) guanine and its triphosphate. Journal of Virology 32:72–77
    [Google Scholar]
  10. Gerber P., Lucas S., Nonoyama M., Perlin E., Goldstein L. 1972; Oral excretion of Epstein-Barr virus by healthy subjects and patients with infectious mononucleosis. Lancet ii:988–989
    [Google Scholar]
  11. Greenspan J. S., Greenspan D., Lennette E., Abrams D. L., Conant M. A., Petersen V., Freese K. 1985; Replication of Epstein-Barr virus within the epithelial cells of oral “hairy” leukoplakia, an AIDS-associated lesion. New England Journal of Medicine 313:1564–1571
    [Google Scholar]
  12. Lin J., Smith C., Pagano J. 1984; Prolonged inhibitory effect of 9(l, 3-dihydroxy-2-propoxy methyl)guanine against replication of Epstein-Barr virus. Journal of Virology 50:50–55
    [Google Scholar]
  13. Miller G., Lipman M. 1973; Comparison of yield of virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus. Journal of Experimental Medicine 138:1398–1411
    [Google Scholar]
  14. Miller G., Niederman J., Andrews B. 1973; Prolonged oropharyngeal excretion of Epstein-Barr virus infectious mononucleosis. New England Journal of Medicine 288:229–232
    [Google Scholar]
  15. Niederman J., Miller G., Pearson G., Pagano I., Dowaliby J. 1976; Infectious mononucleosis: Epstein-Barr virus shedding in the saliva and the oropharynx. New England Journal of Medicine 294:1355–1359
    [Google Scholar]
  16. Pagano J. S., Datta A. K. 1982; Perspectives on interactions of acyclovir. American Journal of Medicine 73: supplement 1A 18–26
    [Google Scholar]
  17. Pagano J., Sixbey J., Lin J. 1983; Acyclovir and Epstein-Barr virus infection. Journal of Antimicrobial Chemotherapy 12: supplement B 113–121
    [Google Scholar]
  18. Reedman B., Klein G. 1973; Cellular localization of an Epstein-Barr virus associated complement fixing antigen in producer and non-producer lymphoblastoid cell lines. International Journal of Cancer 11:499–523
    [Google Scholar]
  19. Schaeffer H. J., Beauchamp L., De Miranda P., Elion G. B., Bauer D. J., Collins P. 1978; 9-(2-hydroxy ethoxymethyl)guanine activity against viruses of the herpes group. Nature, London 272:583–585
    [Google Scholar]
  20. Sixbey I., Nedrud J., Raab-Traub M., Hanes R., Pagano J. 1984; Epstein-Barr virus in oropharyngeal epithelial cells. New England Journal of Medicine 310:1225–1234
    [Google Scholar]
  21. Sullivan J., Medveczky P., Forman S., Baker S., Monroe J., Mulder C., Phil D. 1984; Epstein-Barr virus induced lymphoproliferation: implications for antiviral chemotherapy. New England Journal of Medicine 311:1163–1167
    [Google Scholar]
  22. Wolf H., Haus M., Wilmes E. 1984; Persistence of Epstein-Barr virus in the parotid gland. Journal of Virology 51:795–798
    [Google Scholar]
  23. Yao Q. Y., Rickinson A. B., Epstein M. A. 1985; A re-examination of the Epstein-Barr virus carrier state in healthy seropositive individuals. International Journal of Cancer 35:35–42
    [Google Scholar]
  24. Zerbini M., Ernberg L. 1983; Can Epstein-Barr virus infect and transform all the B-lymphocytes of human cord blood?. Journal of General Virology 64:539–547
    [Google Scholar]
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