The 368-Nucleotide Satellite of Cucumber Mosaic Virus Strain Y from Japan Does Not Cause Lethal Necrosis in Tomato Free

Abstract

Summary

Cucumber mosaic virus (CMV) strain Y from Japan was propagated in Xanthi tobacco. Virus isolated from four successive passages was analysed for its constituent RNAs by polyacrylamide gel electrophoresis (PAGE) under semi-denaturing and fully denaturing conditions. This revealed the initial presence of the 368-nucleotide satellite RNA Y-CARNA 5 (for Y-CMV-associated RNA 5) and the subsequent emergence of a small RNA in the 335-nucleotide size range, which we termed Yn-CARNA 5. The appearance of the smaller Yn-CARNA 5 correlated positively with the increasing tomato necrosis-inducing properties of tissue extracts from the later tobacco passages. After sucrose gradient ultracentrifugation of total CMV-Y RNA, the fraction containing Y-CARNA 5 and Yn-CARNA 5 was subjected to PAGE under semi-denaturing conditions, which provided highly purified gel-eluted preparations of the two CARNA 5 species. When tested for their tomato necrosis-inducing capability in the presence of helper virus genomic RNAs, Y-CARNA 5 was shown to be incapable of eliciting lethal necrosis in Rutgers tomato whereas Yn-CARNA 5 was highly necrogenic.

Keyword(s): CMV , satellite RNA and tomato necrosis
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/content/journal/jgv/10.1099/0022-1317-67-10-2241
1986-10-01
2024-03-29
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References

  1. Ahlquist P., Dasgupta R., Kaesberg P. 1981; Near identity of 3 RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23:183–189
    [Google Scholar]
  2. Avila-Rincon M. J., Collmer C. W., Kaper J. M. 1986; In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of CARNA 5 from cucumber mosaic virus strain S. Virology (in press)
    [Google Scholar]
  3. Clarke P., Lin H-C, Wilcox G. 1982; Ultraviolet imaging: a simple method for detecting nucleic acids in preparative gels. Analytical Biochemistry 124:88–91
    [Google Scholar]
  4. Collmer C. W., Kaper J. M. 1986; Infectious RNA transcripts from cloned cDNAs of cucumber mosaic viral satellites. Biochemical and Biophysical Research Communications 135:290–296
    [Google Scholar]
  5. Collmer C. W., Hadidi A., Kaper J. M. 1985; Nucleotide sequence of the satellite of peanut stunt virus reveals structural homologies with viroids and certain nuclear and mitochondrial introns. Proceedings of the National Academy of Sciences, U.S.A 82:3110–3114
    [Google Scholar]
  6. Garcia-Luque I., Kaper J. M., Diaz-Ruiz J. R., Rubio-Huertos M. 1984; Emergence and characterization of satellite RNAs associated with Spanish cucumber mosaic virus isolates. Journal of General Virology 65:539–547
    [Google Scholar]
  7. Hidaka S., Ishikawa K., Takanami Y., Kubo S., Miura K.-I. 1984; Complete nucleotide sequence of RNA 5 from cucumber mosaic virus (strain Y). FEBS Letters 174:38–42
    [Google Scholar]
  8. Jacquemond M., Lot H. 1981; L’arn satellite du virus de la mosaique du concombre. I. Comparaison de l’aptitude a induire la necrose de la tomate d’ARN satellites isoles de plusieurs souches du virus. Agronomie 1:927–932
    [Google Scholar]
  9. Kaper J. M. 1983; Perspective on CARNA 5, cucumber mosaic virus-dependent replicating RNAs capable of modifying disease expression. Plant Molecular Biology Reporter 1:49–54
    [Google Scholar]
  10. Kaper J. M. 1984; Plant disease regulation by virus-dependent satellite like replicating RNAs. In Control of Virus Diseases pp 317–343 Edited by Kurstak E., Marusyk R. G. New York & Basel: Marcel Dekker;
    [Google Scholar]
  11. Kaper J. M., Waterworth H. E. 1977; Cucumber mosaic virus associated RNA 5: causal agent for tomato necrosis. Science 196:429–431
    [Google Scholar]
  12. Kaper J. M., Tousignant M. E., Lot H. 1976; A low molecular weight RNA associated with a divided genome plant virus: defective or satellite RNA?. Biochemical and Biophysical Research Communications 72:1237–1243
    [Google Scholar]
  13. Kaper J. M., Tousignant M. E., Thompson S. M. 1981; Cucumber mosaic virus-associated RNA 5. VIII. Identification and partial characterization of CARNA 5 incapable of inducing tomato necrosis. Virology 114:526–533
    [Google Scholar]
  14. Marrou J., Duteil M. 1974; La necrose de la tomate. I. Reproduction des symptomes de la maladie par inoculation mecanique de plusieurs souches du virus de la mosaique du concombre (CMV). Annales de Phytopathologie 6:155–171
    [Google Scholar]
  15. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  16. Richards K. E., Jonard G., Jacquemond M., Lot H. 1978; Nucleotide sequence of cucumber mosaic virus-associated RNA 5. Virology 89:395–408
    [Google Scholar]
  17. Takanami Y. 1981; A striking change on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA. Virology 109:120–126
    [Google Scholar]
  18. Yoshida K., Goto T., Iizuka N. 1985; Attenuated isolates of cucumber mosaic virus produced by satellite RNA and cross protection between attenuated isolates and virulent ones. Annals of the Phytopathological Society of Japan 51:238–242
    [Google Scholar]
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