1887

Abstract

Summary

Cellular forms of the DNA of the caulimovirus figwort mosaic virus (FMV), isolated from leaves and callus cells derived from FMV-infected leaf tissue, have been studied. One- and two-dimensional gel electrophoresis and blot hybridization experiments showed the presence of various truncated forms of FMV DNA reminiscent of the putative replication intermediates of cauliflower mosaic virus (CaMV) thought to be generated by a mechanism involving reverse transcription. FMV DNA was found to be qualitatively and quantitatively similar in leaf and callus tissue, suggesting that, in contrast to CaMV, FMV can multiply efficiently in actively proliferating cells cultured .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-10-2227
1986-10-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/10/JV0670102227.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-10-2227&mimeType=html&fmt=ahah

References

  1. Covey S. N. 1985; Organisation and expression of the cauliflower mosaic virus genome. In Molecular Plant Virology vol 2 pp 121–159 Edited by Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  2. Covey S. N., Turner D., Mulder G. 1983; A small DNA molecule containing covalently-linked ribonucleotides originates from the large intergenic region of the cauliflower mosaic virus genome. Nucleic Acids Research 11:251–263
    [Google Scholar]
  3. Donson J., Hull R. 1983; Physical mapping and molecular cloning of caulimovirus DNA. Journal of General Virology 64:2281–2288
    [Google Scholar]
  4. Hohn T., Hohn B., Pfeiffer P. 1985; Reverse transcription in CaMV. Trends in Biochemical Sciences 4:205–209
    [Google Scholar]
  5. Howell S. H. 1985; The molecular biology of plant DNA viruses. CRC Critical Plant Reviews in Plant Sciences 2:287–316
    [Google Scholar]
  6. Hull R., Donson J. 1982; Physical mapping of the DNAs of carnation etched ring and figwort mosaic viruses. Journal of General Virology 60:125–134
    [Google Scholar]
  7. Hull R., Covey S. N. 1983a; Replication of cauliflower mosaic virus DNA. Science Progress, Oxford 68:403–422
    [Google Scholar]
  8. Hull R., Vovey S. N. 1983b; Characterisation of cauliflower mosaic virus DNA forms isolated from infected turnip leaves. Nucleic Acids Research 11:1881–1895
    [Google Scholar]
  9. Marco Y., Howell S. H. 1984; Intracellular forms of viral DNA consistent with a model of reverse transcriptional replication of cauliflower mosaic virus genome. Nucleic Acids Research 12:2655–2660
    [Google Scholar]
  10. Marsh L., Kuzi A., Guilfoyle T. 1985; Identification and characterisation of cauliflower mosaic virus replication complexes - analogy to hepatitis B viruses. Virology 143:212–223
    [Google Scholar]
  11. Maule A. J. 1985; Partial characterisation of different classes of viral DNA, and kinetics of DNA synthesis in turnip protoplasts infected with cauliflower mosaic virus. Plant Molecular Biology 5:25–34
    [Google Scholar]
  12. Maule A. J., Hull R., Donson J. 1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6:215–224
    [Google Scholar]
  13. Murashige T., Skoog F. 1962; A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473–497
    [Google Scholar]
  14. Paszkowski J., Shinshi H., Koenig I., Lazar G. B., Hohn T., Mondak V., Potrykus I. 1983; Proliferation of cauliflower mosaic virus in protoplast derived clones of turnip (Brassica rapa). In Proceedings of the 6th International Protoplast Symposium pp 138–139 Edited by Potrykus I., Harms C. T., Hinnen A., Hutter R., King P. J., Shillito R. D. Basel: Birkauser Verlag;
    [Google Scholar]
  15. Rollo F., Covey S. N. 1985; Cauliflower mosaic virus DNA persists as supercoiled forms in cultured turnip cells. Journal of General Virology 66:603–608
    [Google Scholar]
  16. Shepherd R. J. 1979; DNA plant viruses. Annual Review of Plant Physiology 30:405–423
    [Google Scholar]
  17. Thomas C. M., Hull R., Bryant J. A., Maule A. J. 1985; Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (–) strand viral DNA and reverse transcription of primed RNA templates. Nucleic Acids Research 13:4557–4576
    [Google Scholar]
  18. Turner D. S., Covey S. N. 1984; A putative primer for the replication of cauliflower mosaic virus by reverse transcription is virion associated. FEBS Letters 165285–289
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-10-2227
Loading
/content/journal/jgv/10.1099/0022-1317-67-10-2227
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error