1887

Abstract

Summary

Translation of the two genomic RNAs of a British strain (SP5) of pea early browning virus (PEBV-B) in rabbit reticulocyte lysates produced three principal polypeptides of 165 × 10 (165K), 134K and 23K. The two largest polypeptides were produced by RNA-1 alone, and evidence is presented which suggests that the 165K polypeptide arises from the 134K product by a readthrough event. RNA-2 directed the translation of the 23K polypeptide, which was immunoprecipitated by anti-PEBV-B serum. A polypeptide of 24.3K, produced by translation of the genomic RNAs of the closely related broad bean yellow band virus, was also immunoprecipitated by anti-PEBV-B serum, confirming the serological relationship between the two viruses.

Keyword(s): PEBV , RNA and translation in vitro
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/content/journal/jgv/10.1099/0022-1317-67-10-2125
1986-10-01
2024-04-25
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References

  1. Bergh S. T., Koziel M. G., Huang S. C., Thomas R. A., Gilley D. P., Siegel A. 1985; The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain). Nucleic Acids Research 13:8507–8518
    [Google Scholar]
  2. Bisaro D. M., Siegel A. 1982; Subgenomic components to tobacco rattle virus in infected tissue. Virology 118:411–418
    [Google Scholar]
  3. Boccara M., Hamilton W. D. O., Baulcombe D. C. 1986; The organisation and interviral homologies of genes at the 3′ end of tobacco rattle virus RNA 1. EM BO Journal 5:223–229
    [Google Scholar]
  4. Bordier C., Crettol-Jarvinen A. 1979; Peptide mapping of heterogeneous protein samples. Journal of Biological Chemistry 254:2563–2567
    [Google Scholar]
  5. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  6. Cooper J. I., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  7. Cornelissen B. J. C., Linthorst H. J. M., Brederode F.Th., Bol J. F. 1986; Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2186
    [Google Scholar]
  8. Davies J. W. 1979; Translation of plant virus ribonucleic acids in extracts from eukaryotic cells. In Nucleic Acids in Plants vol 2 pp 111–149 Edited by Hall T. C., Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  9. Davies J. W., Aalbers A. M. J., Stuik E. J., Van Kammen A. 1977; Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ. FEBS Letters 77:265–269
    [Google Scholar]
  10. Forster R. L. S., Morris-Krsinich B. A. M. 1985; Synthesis and processing of the translational products of tobacco ringspot virus in rabbit reticulocyte lysates. Virology 144:516–519
    [Google Scholar]
  11. Franssen H., Moerman M., Rezelman G., Goldbach R. 1984; Evidence that the 32, 000 dalton protein encoded by bottom-component RNA of cowpea mosaic virus is a proteolytic processing enzyme. Journal of Virology 50:183–190
    [Google Scholar]
  12. Fritsch C., Mayo M. A., Hirth L. 1977; Further studies on the translation products of tobacco rattle virus RNA in vitro. Virology 77:722–732
    [Google Scholar]
  13. Harrison B. D. 1966; Further studies on a British form of pea early-browning virus. Annals of Applied Biology 57:121–129
    [Google Scholar]
  14. Harrison B. D., Robinson D. J. 1978; The tobraviruses. Advances in Virus Research 23:25–77
    [Google Scholar]
  15. Hughes G. 1985 The RNA genome of pea early browning virus Ph.D. thesis, University of Birmingham;
    [Google Scholar]
  16. Jen G., Thach R. E. 1982; Inhibition of host translation in encephalomyocarditis virus-infected L cells: a novel mechanism. Journal of Virology 43:250–261
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Mayo M. A., Fritsch C., Hirth L. 1976; Translation of tobacco rattle virus RNA in vitro using wheatgerm extracts. Virology 69:408–415
    [Google Scholar]
  19. Pelham H. R. B. 1978; Leaky UAG termination codon in tobacco mosaic virus RNA. Nature, London 272:469–471
    [Google Scholar]
  20. Pelham H. R. B. 1979; Translation of tobacco rattle virus RNAs in vitro: four proteins from three RNAs. Virology 97:256–265
    [Google Scholar]
  21. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  22. Robinson D. J., Harrison B. D. 1985a; Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses. Journal of General Virology 66:171–176
    [Google Scholar]
  23. Robinson D. J., Harrison B. D. 1985b; Evidence that broad bean yellow band virus is a new serotype of pea early-browning virus. Journal of General Virology 66:2003–2009
    [Google Scholar]
  24. Robinson D. J., Mayo M. A., Fritsch C., Jones A. T., Raschke J. H. 1983; Origin and messenger activity of two small RNA species found in particles of tobacco rattle virus strain SYM. Journal of General Virology 64:1591–1599
    [Google Scholar]
  25. Russo M., Gallitelli D., Vovlas C., Savino V. 1984; Properties of broad bean yellow band virus, a possible new tobravirus. Annals of Applied Biology 105:223–230
    [Google Scholar]
  26. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. 1978; A protein linked to the 5′ termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Research 5:4505–4522
    [Google Scholar]
  27. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:325–330
    [Google Scholar]
  28. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  29. Wodnar-Filipowicz A., Skrzeczowski L. J., Filipowicz W. 1980; Translation of potato virus X RNA into high molecular weight proteins. FEBS letters 109:151–155
    [Google Scholar]
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