1887

Abstract

Summary

Infectivity assays of rice dwarf virus (RDV) were done by the fluorescent antibody focus counting technique on vector cell monolayers of the green rice leafhopper, . The focus count method was shown to be an accurate and quantitative method for determining RDV infectivities. The optimal pH value for inoculation was about 6.0 in a solution containing 0.1 -histidine HCl and 0.01 -MgCl. Below pH 5.5 and above 6.5, the infectivity of RDV dropped rapidly. The optimal adsorption period at 28 °C was dependent upon the RDV concentration. Optimal periods for adsorption with relative RDV concentrations of 10, 10 and 10 were about 60, 90 and 120 min, respectively. The period from virion adsorption to penetration into the cell was about 90 to 120 min. Infective progeny virions were first detected 12 h after the initial inoculation. From 12 to 20 h, the growth rate of the virus in the cells was exponential with a doubling time of about 96 min, and then from 20 to 28 h there was little or no further increase in infective virus. When the infectivities of the same inocula were compared by using the focus count and vector insect injection methods, the dilution endpoints were approx. 10 and 10, respectively. The focus count assay method was thus about 100 times more sensitive than vector injection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-10-2119
1986-10-01
2019-11-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-10-2119
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error