Monoclonal antibodies (MAbs) were elicited with inactivated, purified foot-and-mouth disease virus (FMDV) type O1 strain Brugge (140S) and with 12S protein subunits. Each MAb was tested for its capacity to bind to FMDV O1 Brugge 140S virions, 12S subunits and purified VP1 by radioimmunoassay (RIA) and to neutralize viral infectivity in mouse protection assays. Those MAbs which reacted only with 12S subunits in RIA did not neutralize infectious virus. One MAb, 12FE9.2.1, reacted with 140S, 12S and purified VP1 of FMDV O1 Brugge and neutralized infectious virus. Reactions with different biosynthetic FMDV type O1 Campos VP1 polypeptides localized the binding site of 12FE9.2.1 between amino acid residues 135 and 172. Eight MAbs reacted with both 140S virions and 12S subunits and neutralized infectious virus. Monoclonal antibodies that reacted only with 12S protein subunits or with 140S, 12S and VP1 did not compete in RIA with 140S/12S-reactive neutralizing MAbs for 12S binding sites. The ability of 140S/12S-reactive MAbs to compete for the 12S binding site defined by MAb 10GA4.2.2 was directly related to their capacity to neutralize infectious virus, suggesting that these MAbs were all affecting a single neutralization site. In mouse protection and plaque reduction neutralization assays, 10GA4.2.2 and 12FE9.2.1 were equally effective in neutralizing the homologous FMDV type O1 Brugge, and in cross-neutralization assays both exhibited high titres against four additional strains of type O1 FMDV.
BachrachH. L.1977; Foot-and-mouth disease virus: properties, molecular biology, and immunogenicity. In Beltsville Symposia in Agricultural Research. I. Virology in Agriculture pp 3–22 Edited by
RombergerJ. A.
Montclair, N.J.: Allanheld, Osmun & Co;
BachrachH. L.,
TrautmanR.,
BreeseS. S.1964; Chemical and physical properties of virtually pure foot-and-mouth disease virus. American Journal of Veterinary Research 25:333–342
BachrachH. L.,
MooreD. M.,
MckercherP. D.,
PolatnickJ.1975; Immune and antibody response to isolated capsid protein of foot-and-mouth disease virus. Journal of Immunology 115:1636–1641
BachrachH. L.,
MooreD. M.,
MckercherP. D.,
PolatnickJ.1977; An experimental subunit vaccine for foot-and-mouth disease. Developments in Biological Standardization 35:155–160
BahnemannH. G.1975; Binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production. Archives of Virology 47:47–54
BernardS.,
WantyghemJ.,
GrosclaudeJ.,
LaporteJ.1974; Chromatographic preparation of purified structural proteins from foot-and-mouth disease virus. Biochemical and Biophysical Research Communications 58:624–632
BittleJ. L.,
HoughtenR. A.,
AlexanderH.,
ShinnickT. M.,
SutcliffeJ. G.,
LernerR. A.,
RowlandsD. J.,
BrownF.1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 298:30–33
BrownF.,
SmaleC. J.1970; Demonstration of three specific sites on the surface of foot-and-mouth disease virus by antibody complexing. Journal of General Virology 1:115–127
CowanK. M.1968; Immunochemical studies of foot-and-mouth disease. IV. Preparation and evaluation of antisera specific for virus, virus protein subunit and the infection-associated antigen. Journal of Immunology 101:1183–1191
DimarchiR.,
BrookeG.,
GaleC.,
CracknellV.,
DoelT.,
MowatN.1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
HardyM. M.,
MooreD. M.1981; Neutralization of foot-and-mouth disease virus. I. Sensitization of the 140S virion by antibody also reactive with the 12S protein subunit. Journal of General Virology 55:415–427
HaresnapeJ. M.,
MccahonD.1983; Four independent antigenic determinants on the capsid polypeptides of aphthovirus. Journal of General Virology 64:2345–2355
KaadenO. R.,
AdamK.-H.,
StrohmaierK.1977; Induction of neutralizing antibodies and immunity in vaccinated guinea pigs by cyanogen bromide-peptides of VP3 of foot-and-mouth disease virus. Journal of General Virology 34:397–400
KleidD. G.,
DowbenkoD. J.,
BockL. A.,
HoatlinM. E.,
JacksonM. L.,
PatzerE. J.,
ShireS. J.,
WeddellG. N.,
YansuraD. G.,
MorganD. O.,
MckercherP. D.,
MooreD. M.1985; Production of recombinant vaccines from microorganisms: vaccine for foot-and-mouth disease virus. In Microbiology – 1985 pp 405–408 Edited by
LeiveL.
Washington, D.C.: American Society for Microbiology;
KurzC.,
ForssS.,
KupperH.,
StrohmaierK.,
SchallerH.1981; Nucleotide sequence and corresponding amino-acid sequence of the gene for the major antigen of foot-and-mouth disease virus. Nucleic Acids Research 9:1919–1931
LaporteJ.,
GrosclaudeJ.,
WantyghemJ.,
BernardS.,
RouzeP.1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fièvre aphteuse par des serums provenant de pores immunisés a l’aide d’une proteine virale purifiée. Comptes rendus hebdomadaires des seances de I’Academie des sciences, série D 276:3399–3402
MckercherP. D.,
MooreD. M.,
MorganD. O.,
RobertsonB. J.,
CallisJ. J.,
KleidD. G.,
ShireS. J.,
YansuraD. G.,
DowbenkoD.,
SmallB.1985; Dose-response evaluation of a genetically engineered foot-and-mouth disease virus polypeptide immunogen in cattle. American Journal of Veterinary Research 46:587–590
MakoffA. J.,
PaynterC. A.,
RowlandsD. J.,
BoothroydJ. C.1982; Comparison of the amino acid sequence of the major immunogen from three serotypes of foot and mouth disease virus. Nucleic Acids Research 10:8285–8295
MeloenR. H.,
BriaireJ.,
WoortmeyerR. J.,
Van ZaaneD.1983; The main antigenic determinant detected by neutralizing monoclonal antibodies on the intact foot-and-mouth disease virus particle is absent from isolated VP1. Journal of General Virology 64:1193–1198
MorganD. O.,
MooreD. M.,
MckercherP. D.1980; Vaccination against foot-and-mouth disease. In New Developments with Human and Veterinary Vaccines pp 169–178 Edited by
MizrahiA.,
HertmanI.,
KlingbergM. A.,
KohnA.
New York: Alan R. Liss;
MorganD. O.,
RobertsonB. H.,
MooreD. M.,
TimponeC. A.,
MckercherP. D.1984; Aphthoviruses: control of foot-and-mouth disease with genetic engineering vaccines. In Control of Virus Diseases pp 135–145 Edited by
KurstakE.,
MarusykR. G.
New York: Marcel Dekker;
OuldridgeE. J.,
BarnettP. V.,
ParryN. R.,
SyredA.,
HeadM.,
RweyemamuM. M.1984; Demonstration of neutralizing and non-neutralizing epitopes on the trypsin-sensitive site of foot-and-mouth disease virus. Journal of General Virology 65:203–207
PolatnickJ.,
BachrachH. L.1977; Growth of BHK cells for the production and purification of foot-and-mouth disease virus. In Cell Culture and Its Applications pp 603–615 Edited by
ActonR. T.
New York: Academic Press;
RobertsonB. H.,
MooreD. M.,
GrubmanM. J.,
KleidD. G.1983; Identification of an exposed region of the immunogenic capsid polypeptide VP1 on foot-and-mouth disease virus. Journal of Virology 46:311–316
RobertsonB. H.,
MorganD. O.,
MooreD. M.1984; Location of neutralizing epitopes defined by monoclonal antibodies generated against the outer capsid polypeptide, VP1, of foot-and-mouth disease virus A12. Virus Research 1:489–500
StrohmaierK.,
FranzeR.,
AdamK.-h.1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
SwaneyL. M.,
BishopF. K.,
HouseC. A.1982; A new continuous bovine kidney cell line susceptible to foot-and-mouth disease virus. Abstracts, Annual Meeting of the American Society for Microbiology p 256 Washington, D.C.: American Society for Microbiology;
Vande WoudeG. F.,
SwaneyJ. B.,
BachrachH. L.1972; Chemical and physical properties of foot-and-mouth disease virus: a comparison with Maus-Elberfeld virus. Biochemical and Biophysical Research Communications 48:1222–1229
WagnerG. G.,
CardJ. L.,
CowanK. M.1970; Immunochemical studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease virus concentrated by polyethylene glycol precipitation. Archiv fur die gesamte Virusforschung 30:343–352