%0 Journal Article %A Vela, C. %A Cambra, M. %A Cortés, E. %A Moreno, P. %A Miguet, J. G. %A Pérez De San Román, C. %A Sanz, A. %T Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis %D 1986 %J Journal of General Virology, %V 67 %N 1 %P 91-96 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-67-1-91 %K CTV %K diagnosis %K monoclonal antibodies %I Microbiology Society, %X Summary Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-1-91