1887

Abstract

Summary

Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.

Keyword(s): CTV , diagnosis and monoclonal antibodies
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/content/journal/jgv/10.1099/0022-1317-67-1-91
1986-01-01
2019-11-13
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-1-91
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