1887

Abstract

Summary

Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and used in a double-antibody sandwich enzyme-linked immunosorbent assay for CTV. When tested against 23 strains of CTV, each MAb recognized all the strains with uniform reactions and low background.

Keyword(s): CTV , diagnosis and monoclonal antibodies
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1986-01-01
2024-03-19
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References

  1. Al Moudallal Z., Briand J. P., Van Regenmortel M. H. V. 1982; Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 1:1005–1010
    [Google Scholar]
  2. Bar-Joseph M., Malkinson M. 1980; Hen egg yolk as a source of antiviral antibodies with enzyme-linked immunosorbent assay (ELISA): a comparison of two plant viruses. Journal of Virological Methods 1:179–183
    [Google Scholar]
  3. Bar-Joseph M., Garnsey S. M., Gonsalves D., Moscovitz M., Purcifull D. E., Clark M. F., Loebenstein G. 1979; The use of enzyme-linked immunoassay for detection of citrus tristeza virus. Phytopathology 69:190–194
    [Google Scholar]
  4. Cambra M., Moreno P., Navarro L. 1979; Detectión rápida del virus de la tristeza de los cĺtricos (CTV) mediante la técnica inmunoenzimatica ELISA-sandwich. Anales. Instituto nacional de investigaciones agrarias, serie protección vegetal 12:115–125
    [Google Scholar]
  5. Cambra M., Hermoso De Mendoza A., Moreno P., Navarro L. 1981; Use of enzyme-linked immunosorbent assay (ELISA) for detection of citrus tristeza virus (CTV) in different aphid species. In Proceedings. International Society of Citriculture pp 444–448 Edited by the Editorial Committee (1981), International Citrus Congress Organizing Committee, Japan Chapter ISC; Okitsu, Shimizu, Shizuoka, Japan:
    [Google Scholar]
  6. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  7. Derrick K. S., Brlansky R. H. 1976; Assay for viruses and mycoplasmas using serologically specific electron microscopy. Virology 56:652–653
    [Google Scholar]
  8. Diaco R., Lister R. M., Durand D. P., Hill J. H. 1983; Production of monoclonal antibodies against three isolates of barley yellow dwarf virus. Phytopathology 73:788
    [Google Scholar]
  9. Dietzgen R. G., Sander E. 1982; Monoclonal antibodies against a plant virus. Archives of Virology 74:197–204
    [Google Scholar]
  10. Fishman S., Marcus R., Talpaz H., Bar-Joseph M., Oren Y., Salomon R., Zohar M. 1983; Epidemiological and economic models for spread and control of citrus tristeza virus disease. Phytoparasitica 11:39–49
    [Google Scholar]
  11. Flores R., Garro R., Conejero V., Primo E. 1974; Purificatión de las partículas nucleoprotéicas flexuosas asociadas a la tristeza y ulterior caracterizacion de su ácido nucléico. Revista de agroquímica v tecnología de alimentos 14:278–282
    [Google Scholar]
  12. Garnsey S. M., Gonsalves D., Purcifull D. E. 1978; Rapid diagnosis of citrus tristeza virus infections by sodium dodecyl sulphate-immunodiffusion procedures. Phytopathology 68:88–95
    [Google Scholar]
  13. Garnsey S. M., Christie R. G., Derrick K. S., Bar-Joseph M. 1980; Detection of citrus tristeza virus. II. Light and electron microscopy of inclusions and viral particles. In Proceedings. Eighth Conference of the International Organization of Citrus Virologists (IOCV) pp 9–15 Edited by Calavar C. E., Timmer L. W., Garnsey S. M. Riverside: University of California;
    [Google Scholar]
  14. Garnsey S. M., Bar-Joseph M., Lee R. F. 1981; Applications of serological indexing to develop control strategies for citrus tristeza virus. In Proceedings. International Society of Citriculture pp 448–452 Edited by the Editorial Committee (1981), International Citrus Congress Organizing Committee, Japan Chapter ISC; Okitsu, Shimizu, Shizuoka, Japan:
    [Google Scholar]
  15. Gugerli P., Fries P. 1983; Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. Journal of General Virology 64:2471–2477
    [Google Scholar]
  16. Halk E. L., Hsu H. T., Aebig J., Franke J. 1984; Production of monoclonal antibodies against three ilarviruses and alfalfa mosaic virus and their use in serotyping. Phytopathology 74:367–372
    [Google Scholar]
  17. Hermoso De Mendoza A., Ballester J. F., Pina J. A. 1984; Transmission of citrus tristeza virus by aphids (Homoptera, Aphididae) in Spain. In Proceedings. Ninth Conference of the International Organization of Citrus Virologists (IOCV)23–27 Edited by Garnsey M. S., Timmer L. W., Dodds A. Riverside: University of California;
    [Google Scholar]
  18. Hsu H. T., Aebig J., Rochow W. F. 1984; Differences among monoclonal antibodies to barley yellow dwarf viruses. Phytopathology 74:600–605
    [Google Scholar]
  19. Kearney I. F., Radbruch A., Lesegang B., Rajewsky K. 1979; A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell line. Journal of Immunology 123:1548–1550
    [Google Scholar]
  20. Köhler G., Milstein C. 1975; Continuous cultures of fused cell secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  21. Kronvall G., Greig H. M., Williams R. C. JR 1970; Protein A reactivity with mouse immunoglobulins. Journal of Immunology 105:1116–1122
    [Google Scholar]
  22. Lee R. F., Timmen L. W., Purcifull D. E., Garnsey S. M. 1981; Comparison of radioimmunosorbent assay and enzyme-linked immunosorbent assay for detection of rickettsia-like bacteria and citrus tristeza virus. Phytopathology 71:889
    [Google Scholar]
  23. Moreno P., Cambra M., Navarro L., Fernandez-Montes J., Pina J. A., Ballester J. F., Juarez J. 1980; A survey of citrus tristeza virus (CTV) in the area of Sevilla (Spain) using the ELISA method. In Proceedings. Fifth Congress of the Mediterranean Phytopathological Union (Patras, Greece) pp 41–42 Edited by Panagopoulos G. C., Stathis P., Christias C.
    [Google Scholar]
  24. Müller G. W., Garnsey S. M. 1984; Susceptibility of citrus varieties, species, citrus relatives and non-rutaceous plants to slashcut mechanical inoculation with citrus tristeza virus (CTV). In Proceedings. Ninth Conference of the International Organization of Citrus Virologists (IOCV) pp 33–44 Edited by Garnsey M. S., Timmer L. W., Dodds A. Riverside: University of California;
    [Google Scholar]
  25. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. 1979; The isolation of hybrid cell lines producing monoclonal antibodies against p 15 (E) protein of murine leukemia virus. Virology 93:111–116
    [Google Scholar]
  26. Ouchterlony O., Nilsson L. A. 1978; Immunodiffusion and immunoelectrophoresis. In Handbook of Experimental Immunology pp 19.1–19.44 Edited by Weir D. M. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  27. Sanz A., Barreno B. G., Nogal M., Vinuela E., Enjuanes L. 1985a; Monoclonal antibodies specific for African swine fever virus proteins. Journal of Virology 54:199–206
    [Google Scholar]
  28. Sanz A., Perez De San Roman C., Miguet J. G., Cortes E., Casal L., Cambra M., Vela C. 1985b; Production and characterization of monoclonal antibodies to potato virus Y (PVY) and potato virus X (PVX): application to diagnosis. VI. Reunićn de la Sociedad Espanola de Virologla (Valencia, Espana)
    [Google Scholar]
  29. Tsuchizaki T., Sasaki A., Saito Y. 1978; Purification of citrus tristeza virus diseased fruits and the detection of the virus in citrus tissues by fluorescent antibody techniques. Phytopathology 68:139–142
    [Google Scholar]
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