The extent of homology between the translation products of the HZ2 strain of feline sarcoma virus (HZ2-FeSV) and the Abelson murine leukaemia virus (A-MuLV) was examined immunologically and biochemically. Antiserum prepared against the v-abl-encoded determinants of the A-MuLV polyprotein P120gag-abl was also found to precipitate specifically the 98K mol. wt. HZ2-FeSV protein (P98gag-abl). The basis for this immunological crossreactivity was indicated by the findings that the two proteins had at least six [35S]methionine-containing tryptic peptides and at least eight [35S]methionine-containing chymotryptic peptides in common. Each of the two proteins also had tryptic and chymotryptic peptides which were unique. Both proteins were associated with tyrosyl kinase activities which exhibited some similar biochemical properties in vitro. However, the HZ2-FeSV-associated activity was much more sensitive to competitive inhibition by nucleoside and deoxynucleoside diphosphates than was the A-MuLV-associated activity. These results suggest that, while the gag-abl translation products of these two independent isolates of transforming retrovirus are highly related structurally and functionally, the differences in structure contribute to differences in enzyme activity. Further comparative studies of these two proteins should play an important role in determining their roles in induction of two different types of malignancy: lymphosarcoma in the case of the A-MuLV protein and fibrosarcoma in the case of the HZ2-FeSV protein.
BarbacidM.,
LauverA. V.,
DevareS. G.1980; Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus. Journal of Virology 33:196–207
BesmerP.,
HardyW. D.Jr,
ZuckermanE. E.,
BergoldP.,
LedermanL.,
SnyderH. W.JR1983; The Hardy-Zuckerman 2-FeSV, a new feline retrovirus with oncogene homology to Abelson-MuLV. Nature, London 303:825–828
HardyW. D.Jr,
ZuckermanE. E.,
MarkovichR.,
BesmerP.,
SnyderH. W.JR1982; Isolation of feline sarcoma viruses from pet cats with multicentric fibrosarcomas. In Advances in Comparative Leukemia Virus Research 1981: pp 205–206 Edited by
YohnD.,
BlaskesleeJ. R.
New York: Elsevier/North-Holland;
HendersonI. C.,
LieberM. M.,
TodaroG. J.1974; Mink cell line Mvl Lu (CCL64). Focus formation and the generation of ‘nonproducer’ transformed cell lines with murine and feline sarcoma viruses. Virology 60:282–287
HunterT.,
SeftonB. M.1980; Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proceedings of the National Academy of Sciences, U. S. A 77:1311–1315
RabsteinL. S.,
GazdarA. F.,
ChopraH. C.,
AbelsonH. T.1971; Early morphological changes associated with infection by a murine nonthymic lymphatic tumor virus. Journal of the National Cancer Institute 46:481–491
SeftonB. M.,
HunterT.,
RaschkeW. C.1981; Evidence that the Abelson virus protein functions as a protein kinase that phosphorylates tyrosine. Proceedings of the National Academy of Sciences, U. S. A 78:1552–1556
SnyderH. W.JR,
SinghalM. C.1983; Association of the transforming proteins of the ST and GA strains of feline sarcoma virus and their in vitro-associated protein kinase activities with cellular membranes. Cancer Investigation 1:225–236
StephensonJ. R.,
KhanA. S.,
SliskiA. H.,
EssexM.1977; Feline oncornavirus-associated cell membrane antigen: evidence for animmunologically cross-reactive feline sarcoma virus-coded protein. Proceedings of the National Academy of Sciences, U. S. A 74:5608–5612
Vande VenW. J. M.,
ReynoldsF. H.JR,
StephensonJ. R.1980; The nonstructural components of polyproteins encoded by replication-defective mammalian transforming retroviruses are phosphorylated and have associated protein kinase activity. Virology 101:185–197
WitteO. N.,
RosenbergN.,
BaltimoreD.1979; Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus-encoded PI20 protein at the cell surface. Journal of Virology 31:776–784
WitteO. N.,
DasguptaA.,
BaltimoreD.1980; Abelson murine leukemia virus protein is phosphorylated in vitro to form phosphotyrosine. Nature, London 283:826–831