Identification of Cross-reacting Glycoproteins of Four Herpesviruses by Western Blotting Free

Abstract

SUMMARY

Monospecific rabbit antisera against purified herpes simplex virus type 1 (HSV-1) gB, gC or gD antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (EHV-1)-infected cells was used in Western blotting to identify antigenically related proteins in cells infected with HSV-1, HSV-2, bovine mammillitis virus or EHV-1. Monomeric and oligomeric polypeptides related to HSV-1 gB were found in cells infected with each of the four herpesviruses. The gC antiserum was specific for HSV-1 and the gD antiserum reacted only with polypeptides in HSV-1- or HSV-2-infected cells. Neither gC nor gD antiserum revealed the existence of oligomeric forms of the glycoproteins with the single exception of HSV-1 strain KOS which had a heat-unstable high molecular weight form of gD. The EHV-1 antiserum reacted with high molecular weight polypeptides of the same mobility as the oligomeric form of gB in cells infected with each of the four herpesviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-9-2039
1985-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/9/JV0660092039.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-9-2039&mimeType=html&fmt=ahah

References

  1. Killington R. A., Randall R. E., Yeo J., Honess R. W., Halliburton I. W., Watson D. H. 1977; Observations on antigenic relatedness between viruses of the herpes simplex ‘neutroseron’. In Oncogenesis and Herpesviruses III pp 185–194 Edited by De Thé G., Flenle W. F. Rapp, Lyon: IARC;
    [Google Scholar]
  2. Little S. P., Joffre J. T., Courtney R. J., Schaffer P. A. 1981; A virion-associated glycoprotein essential for infectivity of herpes simplex virus type 1. Virology 115:149–160
    [Google Scholar]
  3. Roberts P. L., Duncan B. E., Raybould T. J. G., Watson D. H. 1985; Purification of herpes simplex virus glycoproteins B and C using monoclonal antibodies and their ability to protect mice against lethal challenge. Journal of General Virology 66:1073–1085
    [Google Scholar]
  4. Sarmiento M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. IV. Conformation of the virion glycoprotein designated VP7 (B2). Journal of Virology 29:1159–1167
    [Google Scholar]
  5. Sarmiento M., Haffey M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7 (B2) in virion infectivity. Journal of Virology 29:1149–1158
    [Google Scholar]
  6. Snowden B. W., Kinchington P. R., Powell K. L., Halliburton I. W. 1985; Antigenic and biochemical analysis of gB of herpes simplex virus type 1 and type 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1. Journal of General Virology 66:231–247
    [Google Scholar]
  7. Vaughan P. J., Purifoy D. J. M., Powell K. L. 1985; DNA-binding protein associated with herpes simplex virus DNA polymerase. Journal of Virology 53:501–508
    [Google Scholar]
  8. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-9-2039
Loading
/content/journal/jgv/10.1099/0022-1317-66-9-2039
Loading

Data & Media loading...

Most cited Most Cited RSS feed