1887

Abstract

Summary

The transcription pattern of II-digested ø3T DNA is described. Eight II fragments that hybridized to transcription products were unequivocally identified. A further hybridizing region corresponding to a II triplet was also revealed, giving a total of nine to 11 II fragments. These represent 47 to 53% of the ø3T genome. Transcription was shown to initiate within II fragments B, G, C, H, I, F and J, implying that all of these contain at least one promoter. The relevance of these data to the construction of a cloning vector based on ø3T is discussed.

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/content/journal/jgv/10.1099/0022-1317-66-9-2029
1985-09-01
2019-11-14
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-9-2029
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