1887

Abstract

SUMMARY

RNA extracted from the rod-shaped particles of Indian peanut clump virus (IPCV), strain L, consisted predominantly of two single-stranded species with mol. wt., estimated by gel electrophoresis of glyoxal-denatured samples, of 1.83 × 10 (RNA-1) and 1.35 × 10 (RNA-2). Both RNA-1 and RNA-2 were needed for lesion production in leaves of cv. Topcrop. Strong nucleotide sequence homologies were detected among three strains of IPCV by nucleic acid hybridization tests. Less strong homologies were found between these Indian isolates and peanut clump virus (PCV) from West Africa. These results, together with similarities in symptomatology, particle size and mode of natural spread, indicate that the Indian isolates are best considered to be strains of PCV. However, no relationship was detected by immunosorbent electron microscopy either between the three Indian strains or between these and the West African strain. Although PCV has properties typical of the proposed furovirus group, no serological relationship was detected between any of the four strains and beet necrotic yellow vein, potato mop-top or soil-borne wheat mosaic viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-9-2011
1985-09-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/9/JV0660092011.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-9-2011&mimeType=html&fmt=ahah

References

  1. Brandes J., Phillippe M. R., Thornberry H. H. 1964; Electron microscopy of particles associated with soil- borne wheat mosaic. Phytopathologische Zeitschrift 50:181–190
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U. S. A 75:4801–4805
    [Google Scholar]
  3. Brosius J., Dull T. J., Noller H. F. 1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences, U. S. A 77:201–204
    [Google Scholar]
  4. Carbon P., Ehresmann C., Ehresmann B., Ebel J. P. 1978; The sequence of Escherichia coli ribosomal 16S RNA determined by new rapid gel methods. FEBS Letters 94:152–156
    [Google Scholar]
  5. Dunn D. B., Hitchborn J. H. 1965; The use of bentonite in the purification of plant viruses. Virology 25:171–192
    [Google Scholar]
  6. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U. S. A 79:5818–5822
    [Google Scholar]
  7. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  8. Harrison B. D., Barker H. 1978; Protease-sensitive structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
    [Google Scholar]
  9. Harrison B. D., Robinson D. J. 1978; The tobraviruses. Advances in Virus Research 23:25–77
    [Google Scholar]
  10. Hsu Y. H., Brakke M. K. 1983; Translation of soil-borne wheat mosaic virus RNAs in rabbit reticulocyte lysate. Phytopathology 73:790
    [Google Scholar]
  11. Hsu Y. H., Brakke M. K. 1985; Sequence relationships among soil-borne wheat mosaic virus RNA species and terminal structures of RNA II. Journal of General Virology 66:915–919
    [Google Scholar]
  12. Kuwata S., Kubo S. 1984; Properties of two O/pWiwm-transmitted viruses: tobacco stunt and lettuce big vein. Sixth International Congress of Virology, Sendai, Japan, 1984 Abstract P4:5–2
    [Google Scholar]
  13. Mayo M. A., Reddy D. V. R. 1985; Translation products of RNA from Indian peanut clump virus. Journal of General Virology 66:1347–1351
    [Google Scholar]
  14. Murant A. F., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  15. Randles J. W., Harrison B. D., Roberts I. M. 1976; Nicotiana velutina mosaic virus: purification, properties and affinities with other rod-shaped viruses. Annals of Applied Biology 84:193–204
    [Google Scholar]
  16. Rao A. S., Brakke M. K. 1969; Relation of soil-borne wheat mosaic virus and its fungal vector. Polymyxa graminis. Phytopathology 59:581–587
    [Google Scholar]
  17. Reddy D. V. R., Rajeshwari R., Iizuka W., Lesemann D. E., Nolt B. L., Goto T. 1983; The occurrence of Indian peanut clump, a soil-borne virus disease of groundnuts (Arachis hypogaea) in India. Annals of Applied Biology 102:305–310
    [Google Scholar]
  18. Roberts I. M. 1980; A method for providing comparative counts of small particles in electron microscopy. Journal of Microscopy 118:241–245
    [Google Scholar]
  19. Roberts I. M., Harrison B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  20. Robinson D. J., Harrison B. D. 1985; Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses. Journal of General Virology 66:171–176
    [Google Scholar]
  21. Robinson D. J., Barker H., Harrison B. D., Mayo M. A. 1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
    [Google Scholar]
  22. Shirako Y., Brakke M. K. 1984; Two purified RNAs of soil-borne wheat mosaic virus are needed for infection. Journal of General Virology 65:119–127
    [Google Scholar]
  23. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  24. Thouvenel J. C., Fauquet C. 1981a; Further properties of peanut clump virus and studies on its natural transmission. Annals of Applied Biology 97:99–107
    [Google Scholar]
  25. Thouvenel J. C., Fauquet C. 1981b; Peanut clump virus. Commonwealth Mycological Institute I Association of Applied Biologists Descriptions of Plant Viruses no. 235
    [Google Scholar]
  26. Thouvenel J. C., Germani G., Pfeiffer P. 1974; Preuve de l’origine virale de rabougrissement ou “clump” de l’arachide en Haute-Volta et au Senegal. Comptes rendus hebdomadaires des seances de I’Academie des sciences, serie D 278:2847–2849
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-9-2011
Loading
/content/journal/jgv/10.1099/0022-1317-66-9-2011
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error