1887

Abstract

SUMMARY

Mycoviruses from (ATCC 28706) (AoV) and (ATCC 14586) (PsV) were isometric, had diameters of 30 to 32 nm and 32 to 34 nm respectively, and resolved into six bands in CsCl at buoyant densities of 1.275 to 1.357 and 1.285 to 1.390 g/cm, respectively. All properties of PsV were the same as previous reports except an additional RNA segment with a mol. wt. of 0.51 × 10 and a reverse in the ratio of the major and minor capsid polypeptides. The AoV complex contained nine segments of dsRNA with mol. wt. ranging from 1.12 × 10 to 0.38 × 10, and five thermomelting transition points between 76 and 88°C. It contained capsid polypeptides with mol. wt. of 60000, 51000 and 41000. One dsRNA segment (mol. wt. 1.10 × 10) and one capsid polypeptide (mol. wt. 51000) were common to AoV and PsV-S. Serological tests and virus electrophoresis indicated that at least two serologically distinct viruses were present in . In double-diffusion serological tests one serological component of AoV and PsV reacted with antiserum of the other virus complex, indicating a serological relationship. A P-5′-end-labelled probe of AoV RNA hybridized with PsV RNA and a labelled PsV probe hybridized with AoV RNA. It was concluded that one viral component of the AoV complex is related to the PsV-S component of the PsV complex.

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1985-09-01
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References

  1. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., Hammelweit F., Ratti G., Sharpe T. J., Planterose D. N. 1970; Antiviral activity of double stranded RNA from a virus isolated from Aspergillus foetidus. Nature London 227:505–507
    [Google Scholar]
  2. Bozarth R. F., Chow C. C. 1966; Pea enation mosaic virus: purification and properties. Contributions. Boyce Thompson Institute for Plant Research 23:301–309
    [Google Scholar]
  3. Bozarth R. F., Harley E. H. 1976; The electrophoretic mobility of double-stranded RNA in polyacrylamide gels as a function of molecular weight. Biochimica et biophysica acta 432:329–335
    [Google Scholar]
  4. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  5. Bozarth R. F., Wood H. A., Goenaga A. 1972; Virus-like particles from a culture of Diplocarpon rosae. Phytopathology 62:493
    [Google Scholar]
  6. Bozarth R. F., Koltin Y., Weissman M. B., Parker R. L., Dalton R. E., Steinlauf R. 1981; The molecular weight and packaging of dsRNAs in the mycovirus from Ustilago maydis killer strains. Virology 113:492–502
    [Google Scholar]
  7. Buck K. W., Girvan R. F. 1977; Comparison of the biophysical and biochemical properties of Penicillium cyaneo-fulvum virus and Penicillium chrysogenum virus. Journal of General Virology 34:145–154
    [Google Scholar]
  8. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  9. Buck K. W., Kempson-Jones G. F. 1974; Capsid polypeptides of two viruses isolated from Penicillium stoloniferum. Journal of General Virology 22:44–445
    [Google Scholar]
  10. Buck K. W., Ratti G. 1975; Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. Journal of General Virology 27:211–224
    [Google Scholar]
  11. Buck K. W., Girvan R. F., Ratti G. 1973; Two serologically distinct double-stranded ribonucleic acid viruses isolated from Aspergillus niger. Transactions, Biochemical Society 1:1138–1140
    [Google Scholar]
  12. Chater K. F., Morgan D. H. 1974; Ribonucleic acid synthesis by isolated viruses of Penicillium stoloniferum. Journal of General Virology 24:307–317
    [Google Scholar]
  13. Diener T. O., Schneider I. R. 1968; Virus degradation and nucleic acid release in single-phase phenol system. Archives of Biochemistry and Biophysics 124:401–412
    [Google Scholar]
  14. Dische Z. 1955; Color reaction of nucleic acid components. In The Nucleic Acids vol 1: pp 285–305 Edited by Chargaff E., Davidson J. N. New York: Academic Press;
    [Google Scholar]
  15. Field L. J., Bruenn J. A., Chang T. 1983; Two Ustilago maydis viral dsRNAs of different size code for the same product. Nucleic Acids Research 11:2765–2778
    [Google Scholar]
  16. Geelen J. L. M. C., Rezelman G., Van Kammen A. 1973; The infectivity of the two electrophoretic forms of cowpea mosaic virus. Virology 51:279–286
    [Google Scholar]
  17. Howley P. M., Israel M. K., Law M. F., Martin M. A. 1979; A rapid method for detecting and mapping homology between heterologous DNAs. Journal of Biological Chemistry 254:4876–4883
    [Google Scholar]
  18. Jordan R. L., Dodds J. A. 1983; Hybridization of 5′-end-labelled RNA to plant viral RNA in agarose and acrylamide gels. Plant Molecular Biology Report 1:31–37
    [Google Scholar]
  19. Kong T. 1979 Biophysical and biochemical characteristics of viruslike particles in Aspergillus ochraceous Master’s thesis Indiana State University;
    [Google Scholar]
  20. Lhoas P. 1971; Transmission of double-stranded RNA viruses to a strain of Penicillium stoloniferum through heterokaryosis. Nature, London 230:248–249
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning pp 122:173–177 200–201 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Ratti G., Buck K. W. 1972; Virus particles in Aspergillus foetidus: a multicomponent system. Journal of General Virology 14:165–175
    [Google Scholar]
  23. Stark W. M., Kleinschmidt W. J., Probst G. W. 1963; Statolon production by submerged culture fermentation. Patent No 3:108–047
    [Google Scholar]
  24. Thiele D. J., Hannig E. M., Leibowitz M. J. 1984; Multiple L double-stranded RNA species of Saccharomyces cerevisiae: evidence for separate encapsidation. Molecular and Cellular Biology 4:92–100
    [Google Scholar]
  25. Weber K., Pringle C. R., Osborn N. 1972; Measurement of molecular weight by electrophoresis on SDS–acrylamide gel. Methods in Enzymology 16:3–27
    [Google Scholar]
  26. Wood H. A., Bozarth R. F. 1972; Properties of virus-like particles of Penicillium chrysogenum: one doublestranded RNA molecule per particle. Virology 47:604–609
    [Google Scholar]
  27. Wood H. A., Bozarth R. F., Mislivec P. B. 1971; Virus-like particles associated with an isolate of Penicillium breci-compactum. Virology 44:592–598
    [Google Scholar]
  28. Wood H. A., Bozarth R. F., Adler J., Mackenzie D. W. 1973; Proteinaceous virus-like particles from an isolate of Aspergillus fiavus. Journal of Virology 13:532–534
    [Google Scholar]
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