1887

Abstract

SUMMARY

The episomal copies of Epstein-Barr virus (EBV) DNA are in a chromatin structure in the lymphoblastoid cell line B95-8. Nucleosomes on EBV DNA have the same spacing as those in cellular chromatin. Some of the EBV DNA is not in nucleosomes; this probably corresponds to DNA which is being packaged. Several DNase hypersensitive sites have been mapped on the EBV genome and patterns of CpG methylation are examined.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-9-1931
1985-09-01
2022-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/9/JV0660091931.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-9-1931&mimeType=html&fmt=ahah

References

  1. Baer B., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature, London 310:207–211
    [Google Scholar]
  2. Ciejeck E. M., Tsai M.-J., O’malley B. W. 1983; Actively transcribed genes are associated with the nuclear matrix. Nature, London 306:607–609
    [Google Scholar]
  3. Cook P. R., Lang J., Hayday A., Lania L., Fried M., Chiswell D. J., Wyke I. A. 1982; Active viral genes in transformed cells lie dose to the nuclear cage. EMBO Journal 1:447–452
    [Google Scholar]
  4. Diala E. S., Hoffman R. M. 1983; Epstein-Barr HR-1 virion DNA is very highly methylated. Journal of Virology 45:482–483
    [Google Scholar]
  5. Dyson P. J., Rabbitts T. H. 1985; Chromatin structure around the c-myc gene in Burkitt’s lymphoma cells with upstream and downstream translocation points. Proceedings of the National Academy of Sciences, U. S. A 82:1984–1988
    [Google Scholar]
  6. Dyson P. J., Cook P. R., Searle S., Wyke J. A. 1985; The chromatin structure of Rous sarcoma proviruses is changed by factors that act in trans in cell hybrids. EMBO Journal 4:413–420
    [Google Scholar]
  7. Edwards P. A. W., Smith C. M., Neville A. M., O’hare M. J. 1982; A human-human hybridoma system based on a fast growing mutant of the ARH-77 plasma cell leukaemia-derived line. European Journal of Immunology 12:641–648
    [Google Scholar]
  8. Elgin S. C. R. 1981; DNAase 1-hypersensitive sites of chromatin. Cell 27:413–415
    [Google Scholar]
  9. Farrell P. J., Bankier A., Seguin C., Deininger P., Barrell B. G. 1983a; Latent and lytic cycle promoters of Epstein-Barr virus. EMBO Journal 2:1331–1338
    [Google Scholar]
  10. Farrell P. J., Deininger P. L., Bankier A., Barrell B. 1983b; Homologous upstream sequences near Epstein-Barr virus promoters. Proceedings of the National Academy of Sciences, U. S. A 80:1656–1669
    [Google Scholar]
  11. Felsenfeld G., Mcghee J. 1982; Methylation and gene control. Nature, London 296:602–603
    [Google Scholar]
  12. Flint S. J., Weintraub H. M. 1977; An altered subunit configuration associated with the actively transcribed DNA of integrated adenovirus genes. Cell 12:783–794
    [Google Scholar]
  13. Griffin B. E., Karran L. 1984; Immortalisation of monkey epithelial cells by specific fragments of Epstein- Barr virus. Nature, London 309:78–82
    [Google Scholar]
  14. Laemmli U. K., Cheng S. M., Adolph K. W., Paulson J. R., Brown J. A., Baumbach W. R. 1977; Metaphase chromosome structure; the role of nonhistone chromosomal proteins. Cold Spring Harbor Symposia on Quantitative Biology 42:351–360
    [Google Scholar]
  15. Leinbach S. S., Summers W. C. 1980; The structure of herpes simplex virus type 1 DNA as probed by micrococcal nuclease digestion. Journal of General Virology 51:45–59
    [Google Scholar]
  16. Lindahl T., Adams A., Bjursell G., Bornkamm W., Kaschka-Dierich C. 1976; Covalently closed circular duplex DNA of EBV in a human lymphoid cell line. Journal of Molecular Biology 102:511–530
    [Google Scholar]
  17. Mcghee J. D., Wood W. L., Dolan M., Engel L. D., Felsenfeld G. 1981; A 200 base pair region at the 5′ end of the chicken adult jl globin gene is accessible to nuclease digestion. Cell 27:45–55
    [Google Scholar]
  18. Miller G., Shope T., Lisco H., Stitt D., Lipman M. 1972; Epstein-Barr virus: transformation, cytopathic changes and viral antigens in squirrel monkey and marmoset leukocytes. Proceedings of the National Academy of Sciences, U. S. A 69:383–387
    [Google Scholar]
  19. Mirkovitch J., Mirault M.-E., Laemmli U. K. 1984; Organisation of the higher order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39:223–232
    [Google Scholar]
  20. Nonoyama M., Pagano J. 1972; Separation of Epstein-Barr virus DNA from large chromosomal DNA in non virus producing cells. Nature New Biology 238:169–171
    [Google Scholar]
  21. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  22. Robinson S. I., Nelkin B. D., Vogelstein B. 1982; The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Cell 28:99–106
    [Google Scholar]
  23. Roizman B. (editor) 1982 The Herpesviruses vol 1: New York: Plenum Press;
    [Google Scholar]
  24. Saragosti S., Moyne G., Yaniv M. 1980; Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA. Cell 20:65–73
    [Google Scholar]
  25. Shaw J. E. 1985; The circular intracellular form of Epstein-Barr virus DNA is amplified by the virus associated DNA polymerase. Journal of Virology 53:1012–1015
    [Google Scholar]
  26. Shaw J. E., Levinger L. F., Carter C. W. 1979; Nucleosomal structure of Epstein-Barr virus DNA in transformed cell lines. Journal of Virology 29:657–665
    [Google Scholar]
  27. Siebenlist U., Hennighausen L., Battey J., Leder P. 1984; Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt’s lymphoma. Cell 37:381–391
    [Google Scholar]
  28. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  29. Summers W. C., Klein G. 1976; Inhibition of Epstein-Barr virus DNA synthesis and late gene expression by phosphonoacetic acid. Journal of Virology 18:151–155
    [Google Scholar]
  30. Varshavsky A. J., Sundin O., Bohn M. 1979; A stretch of late SV40 viral DNA about 400bp long which includes the origin of replication is specifically exposed in SV40 minichromosomes. Cell 16:453–466
    [Google Scholar]
  31. Zur Hausen H., Hecker E., O’neill F. J., Freese U. K. 1978; Persistent oncogenic herpesvirus induced by the tumour promoter TPA. Nature, London 212:373–375
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-9-1931
Loading
/content/journal/jgv/10.1099/0022-1317-66-9-1931
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error