1887

Abstract

SUMMARY

Evidence of multiple genotypes of bluetongue virus (BTV) serotype 11 simultaneously infecting individual cattle was demonstrated in a California sentinel herd naturally infected with two BTV serotypes (11 and 17). Monitoring of weekly virus isolates by PAGE demonstrated genome segment diversity among BTV serotype 11 isolates and an individual bull simultaneously infected with multiple electropherotypes of this serotype. No electrophoretic variations were apparent in BTV serotype 17 isolates. The results of this study indicate that multiple plaque clonings and comparative electrophoresis are required to analyse BTV field isolates accurately for the presence of mixed infections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-9-1901
1985-09-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/9/JV0660091901.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-9-1901&mimeType=html&fmt=ahah

References

  1. Barber T. L., Collisson E. W. 1983; Implications of a new bluetongue serotype for the U,. S. livestock industry. Proceedings, 87th Annual Meeting of the United States Animal Health Association90–104
    [Google Scholar]
  2. Barber T. L., Jochim M. M. 1975; Serotyping bluetongue and epizootic hemorrhagic disease virus strains. Proceedings, 18th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians149–162
    [Google Scholar]
  3. Beards G. M. 1982; Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Archives of Virology 74:65–70
    [Google Scholar]
  4. Bohl E. H., Theil K. W., Saif L. J. 1984; Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. Journal of Clinical Microbiology 19:105–111
    [Google Scholar]
  5. Clarke I. N., McCrae M. A. 1982; Structural analysis of electrophoretic variation in genome profiles of rotavirus field isolates. Infection and Immunity 36:492–497
    [Google Scholar]
  6. Espejo R. T., Puerto F. 1984; Shifts in the electrophoretic pattern on the RNA genome of rotaviruses under different electrophoretic conditions. Journal of Virological Methods 8:293–299
    [Google Scholar]
  7. Gorman B. M. 1979; Variation in orbiviruses. Journal of General Virology 44:1–15
    [Google Scholar]
  8. Gorman B. M., Taylor J. 1978; The RN A genome of Tilligerry virus. Australian Journal of Experimental Biology and Medical Science 56:359–367
    [Google Scholar]
  9. Gorman B. M., Walker P. J., Taylor J. 1977; Electrophoretic separation of double-stranded RNA genome segments from Warrego and Mitchell River viruses. Archives of Virology 54:153–158
    [Google Scholar]
  10. Gorman B. M., Taylor J., Walker P. J., Young P. R. 1978; The isolation of recombinants between related orbiviruses. Journal of General Virology 41:333–342
    [Google Scholar]
  11. Gorman B. M., Taylor J., Walker P. J., Davidson W. L., Brown F. 1981; Comparison of bluetongue type 20 with certain viruses of the bluetongue and Eubenangee serological groups of orbiviruses. Journal of General Virology 57:251–261
    [Google Scholar]
  12. Gorman B. M., Taylor J., Finnimore P. M., Bryant J. A., Sangar D. V., Brown F. 1982; A comparison of bluetongue viruses isolated in Australia with exotic bluetongue virus serotypes. In Arbovirus Research in Australia pp 101–109 Edited by St George T. D., Kay B. H. Brisbane: Division of Animal Health, Commonwealth Scientific and Industrial Research Organization and Queensland Institute of Medical Research;
    [Google Scholar]
  13. Gorman B. M., Taylor J., Walker P. J. 1983; Orbiviruses. In The Reoviridae pp 287–357 Edited by Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  14. GouveA V. S., Schnitzer T. J. 1982; Polymorphism of the migration of double-stranded RNA genome segments of avian reoviruses. Journal of Virology 43:465–471
    [Google Scholar]
  15. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. 1982; Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. Journal of Clinical Microbiology 16:473–477
    [Google Scholar]
  16. Hrdy D. B., Rosen L., Fields B. N. 1979; Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle and mice. Journal of Virology 31:104–111
    [Google Scholar]
  17. Huismans H., Bremer C. W. 1981; A comparison of an Australian bluetongue virus isolate (CSIR019) with other bluetongue virus serotypes by cross-hybridization and cross-immune precipitation. Onderstepoort Journal of Veterinary Research 48:59–67
    [Google Scholar]
  18. Kahlon J., Sugiyama K., Roy P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  19. Knudson D. L., Butterfield W. K., Shope R. E., Walton T. E., Campbell C. H. 1982; Electrophoretic comparison of the genomes of North American bluetongue viruses, one Australian bluetongue virus, and three other related orbiviruses. Veterinary Microbiology 7:285–293
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  21. Lourenco M. H., Nicolas J. C., Cohen J., Scherrer R., Bricout F. 1981; Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolated in France. Annals of Virology 132E:161–173
    [Google Scholar]
  22. Luedke A. J., Jochim M. M., Bowne J. G., Jones R. H. 1970; Observations on latent bluetongue virus infection in cattle. Journal of the American Veterinary Medical Association 156:1871–1879
    [Google Scholar]
  23. Martin S. A., Zweerink H. J. 1972; Isolation and characterization of two types of bluetongue virus particles. Virology 50:495–506
    [Google Scholar]
  24. Nicolas J. C., Pothier P., Cohen J., Lourenco M. H., Thompson R., Guimbaud P., Chenon A., Dauvergne M., Bricout F. 1984; Survey of human rotavirus propagation as studied by electrophoresis of genomic RNA. Journal of Infectious Diseases 149:688–693
    [Google Scholar]
  25. Osburn B. I., McGowan B., Heron B., Loomis E., Bushnell R., Stott J., Utterback W. 1981; Epizootiologic study of bluetongue: virologic and serologic results. American Journal of Veterinary Research 42:884–887
    [Google Scholar]
  26. Owen N. C., Dutolt R. M., Howell P. G. 1965; Bluetongue in cattle: typing of viruses isolated from cattle exposed to natural infections. Onderstepoort Journal of Veterinary Research 32:3–6
    [Google Scholar]
  27. Pedley S., Hundley F., Chrystie I., Mccrae M. A., Desselberger U. 1984; The genomes of rotaviruses isolated from chronically infected immunodeficient children. Journal of General Virology 65:1141–1150
    [Google Scholar]
  28. Pereira H. G., Azeredo R. S., Fialho A. M., Vidal M. N. P. 1984; Genomic heterogeneity of simian rotavirus SAIL. Journal of General Virology 65:815–818
    [Google Scholar]
  29. Ramig R. F., Cross R. K., Fields B. N. 1977; Genome RNAs and polypeptides of reovirus serotypes 1, 2 and 3. Journal of Virology 22:726–733
    [Google Scholar]
  30. Rodriguez W. J., Kim H. W., Brandt C. D., Gardner M. K., Parrott R. H. 1983; Use of electrophoresis of RNA from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. Journal of Infectious Diseases 148:34–40
    [Google Scholar]
  31. Sabara M., Deregt D., Babiuk L. A., Misra V. 1982; Genetic heterogeneity within individual bovine rotavirus isolates. Journal of Virology 44:813–822
    [Google Scholar]
  32. Sammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135–141
    [Google Scholar]
  33. Schroeder B. A., Street J. E., Kalmakoff J., Bellamy A. R. 1982; Sequence relationships between the genome segments of human and animal rotavirus strains. Journal of Virology 43:379–385
    [Google Scholar]
  34. Shatkin A. J., Sipe J. D., Loh P. 1968; Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2:986–991
    [Google Scholar]
  35. Spencer E. G., Avendano L. F., Garcia B. I. 1983; Analysis of human rotavirus mixed electropherotypes. Infection and Immunity 39:569–574
    [Google Scholar]
  36. Squire K. R. E., Chuang R. Y., Osburn B. I., Knudson D. L., Doi R. H. 1983a; Rapid methods for comparing the double-stranded RNA genome profiles of bluetongue virus. Veterinary Microbiology 8:543–553
    [Google Scholar]
  37. Squire K. R. E., Osburn B. I., Chuang R. Y., Doi R. H. 1983b; A survey of electropherotype relationships of bluetongue virus isolates from the western United States. Journal of General Virology 64:2103–2115
    [Google Scholar]
  38. Stott J. L., Barber T. L., Osburn B. I. 1978; Serotyping bluetongue virus: a comparison of plaque inhibition (disc) and plaque neutralization methods. Proceedings, 21st Annual Meeting of the American Association of Veterinary Diagnosticians399–410
    [Google Scholar]
  39. Stott J. L., Osburn B. I., Barber T. L. 1982; Recovery of dual serotypes of bluetongue virus from infected sheep and cattle. Veterinary Microbiology 7:197–207
    [Google Scholar]
  40. Stott J. L., Bushnell R. B., Loomis E. C., Jessup D., O’Rourke M., Oliver M. N., Osburn B. I., Walton T. E. 1983; Overview of a longitudinal field study of bluetongue virus infection in resident populations of Culicoides variipennis and a multispecies sentinel herd. In Double-stranded RNA Viruses pp 367–374 Edited by Compans R. W., Bishop D. H. L. New York: Elsevier;
    [Google Scholar]
  41. Sugiyama K., Bishop D. H. L., Roy P. 1981; Analyses of the genomes of bluetongue viruses recovered in the United States. Virology 114:210–217
    [Google Scholar]
  42. Sugiyama K., Bishop D. H. L., Roy P. 1982; Analyses of the genomes of bluetongue viruses recovered from different states of the United States and at different times. American Journal of Epidemiology 115:332–347
    [Google Scholar]
  43. Verwoerd D. W., Huismans H. 1972; Studies of the in vitro and the in vivo transcription of the bluetongue virus genome. Onderstepoort Journal of Veterinary Research 39:185–192
    [Google Scholar]
  44. Verwoerd D. W., Louw H., Oellerman R. A. 1970; Characterization of bluetongue virus ribonucleic acid. Journal of Virology 5:1–7
    [Google Scholar]
  45. WalkeR P. J., Mansbridge J. N., Gorman B. M. 1980; Genetic analysis of orbiviruses by using RNase T, oligonucleotide fingerprints. Journal of Virology 34:583–591
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-9-1901
Loading
/content/journal/jgv/10.1099/0022-1317-66-9-1901
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error