1887

Abstract

SUMMARY

Purified preparations of peanut stunt virus-associated RNA 5 (PARNA 5) and cucumber mosaic virus-associated RNA 5 (CARNA 5) were used to inoculate cowpea protoplasts, alone or together with their respective helper viruses. After incubation of the protoplasts for up to 5 days, total nucleic acid was extracted, denatured, electrophoresed, transferred to nitrocellulose membranes and hybridized to cDNA probes specific for satellite and viral RNA. Satellite replication could be detected only in protoplasts infected with mixtures of satellite and viral RNA, not in those inoculated with satellite RNA alone. These results suggest that the dependence of cucumovirus satellite RNAs on their helpers is on the replication function rather than on the spreading and/or encapsidation functions of the helper virus.

Keyword(s): CARNA 5 , PARNA 5 and satellite RNAs
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-8-1839
1985-08-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/8/JV0660081839.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-8-1839&mimeType=html&fmt=ahah

References

  1. Jones A. T., Mayo M. A. 1984; Satellite nature of the viroid-like RNA-2 of Solanum nodiflorum mottle virus and the ability of other plant viruses to support the replication of viroid-like RN A molecules. Journal oj General Virology 65:1713–1721
    [Google Scholar]
  2. Kaper J. M., Tousignant M. E., Diaz-ruiz J. R., Tolin S. A. 1978; Peanut stunt virus-associated RNA 5: second tripartite genome virus with an associated satellite-like replicating RNA. Virology 88:166–170
    [Google Scholar]
  3. Linthorst H. J. M., Kaper J. M. 1984a; Circular satellite-RNA molecules in satellite of tobacco ringspot virus-infected tissue. Virology 137:206–210
    [Google Scholar]
  4. Linthorst H. J. M., Kaper J. M. 1984b; Replication of peanut stunt virus and its associated RNA 5 in cowpea protoplasts. Virology 139:317–329
    [Google Scholar]
  5. Murant A. F., Mayo M. A. 1982; Satellites of plant viruses. Annual Review of Phytopathology 20:49–70
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-66-8-1839
Loading
/content/journal/jgv/10.1099/0022-1317-66-8-1839
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error