Molecular Cloning of cDNA Encoding gp68 of Adult T-Cell Leukaemia-associated Antigen: Evidence for Expression of the pX IV Region of Human T-Cell Leukaemia Virus
A 2.3 kb cDNA was cloned from human T-cell leukaemia virus [HTLV(MT-2)] virion RNA using a vector system, as plasmid pHTLV 707. The restriction endonuclease map of pHTLV 707 revealed that the insert contained the 5′ half of the env gene and a portion of the pX region of HTLV, corresponding to the subgenomic RNA derived from 32S defective HTLV. Nucleotide sequence analysis of pHTLV 707 indicated that the clone contained an open reading frame for a 60K mol. wt. protein including the upstream and entire pX IV region. A rabbit antibody raised against a synthetic decapeptide deduced from the nucleotide sequence at the carboxyl terminus of the pX IV region immunoprecipitated gp68, and also 80K and 40K proteins.
HaseltineW. A.,
SodroskiJ.,
PatarcaR.,
BriggsD.,
PerkinsD.,
Wong-StaalF.1984; Structure of 3′ terminal region of type II human T lymphotropic virus: evidence for new coding region. Science 225:419–421
KiyokawaT.,
SeikiM.,
ImagawaK.,
ShimizuF.,
YoshidaM.1984; Identification of a protein (p40x) encoded by a unique sequence pX of human T-cell leukemia virus type I. Gann 75:747–751
KobayashiN.,
KoyanagiY.,
YamamotoN.,
HinumaY.,
SatoH.,
OkochiK.,
HatanakaM.1984c; 28, 000-dalton polypeptide (p28) of adult T-cell leukemia-associated antigen encoded by 24S mRNA of human T-cell leukemia virus has an associated protein kinase activity. Journal of Biological Chemistry 259:11162–11164
KoyanagiY.,
HinumaY.,
SchneiderJ.,
ChosaT.,
HunsmannG.,
KobayashiN.,
HatanakaM.,
YamamotoN.1984; Expression of HTLV-specific polypeptides in various human T-cell lines. Medical Microbiology and Immunology 173:127–140
LeeT. H.,
CouganJ. E.,
SodroskiJ. G.,
HaseltineW. A.,
SalahuddinS. Z.,
Wong-StaalF.,
GalloR. C.,
EssexM.1984; Antigens encoded by the 3′-terminal region of human T-cell leukemia virus: evidence for a functional gene. Science 226:57–61
MiwaM.,
ShimotohnoK.,
HoshinoH.,
FujinoM.,
SugimuraT.1984; Detection of pX proteins in human T-cell leukemia virus (HTLV)-infected cells by using antibody against peptide deduced from sequences of X-IV DNA of HTLV-I and Xc DNA of HTLV-II proviruses. Gann 75:752–755
MiyoshiI.,
KubonishiI.,
YoshimotoS.,
ShiraishiY.1981; A novel T-cell line derived from normal human cord leukocytes by co-culturing with human leukemic T-cells. Gann 72:978–981
SchneiderJ.,
YamamotoN.,
HinumaY.,
HunsmannG.1984; Sera from adult T-cell leukemia patients react with envelope and core polypeptides of adult T-cell leukemia virus. Virology 132:1–11
SeikiM.,
HattoriS.,
HirayamaY.,
YoshidaM.1983; Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proceedings of the National Academy of Sciences, U. S. A 80:3618–3622
ShimotohnoK.,
GoldeD. W.,
MiwaM.,
SagimuraT.,
ChenI. S. Y.1984; Nucleotide sequence analysis of the long terminal repeat of human T-cell leukemia virus type II. Proceedings of the National Academy of Sciences, U. S. A 81:1079–1083
SlamonD. J.,
ShimotohnoK.,
ClineM. I.,
GoldeD. W.,
ChenI. S. Y.1984; Identification of the putative transforming protein of the human T-cell leukemia viruses HTLV-I and HTLV-II. Science 226:61–65
SodroskiJ. G.,
RosenC. A.,
HaseltineW. A.1984; Trans-acting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells. Science 225:381–385
SugamuraK.,
FujiiM.,
KannagiM.,
SakitaniM.,
TakeuchiM.,
HinumaY.1984; Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T-cell leukemia virus. International Journal of Cancer 34:221–228
YamamotoN.,
SchneiderJ.,
KoyanagiY.,
HinumaY.,
HunsmannG.1983; Adult T-cell leukemia (ATL) virus-specific antibodies in ATL patients and healthy virus carriers. International Journal of Cancer 32:281–287
Molecular Cloning of cDNA Encoding gp68 of Adult T-Cell Leukaemia-associated Antigen: Evidence for Expression of the pX IV Region of Human T-Cell Leukaemia Virus