1887

Abstract

SUMMARY

Varicella-zoster virus (VZV)-infected cell surface proteins were investigated using extrinsic radiolabelling of the cell surface, immunoprecipitation of detergent-solubilized extract of the same cell surface and fractionation of the immunoprecipitates using SDS-PAGE. Glycosylated proteins were identified by their affinity for lectin. Six glycoproteins with apparent mol. wt. of 170K, 105K, 93K, 81K, 53K and 45K were identified. The 170K glycoprotein was shown to be disulphidelinked since under reducing conditions for SDS-PAGE it was cleaved to a protein of 63K mol. wt. The IgG responses to these glycoproteins during various clinical circumstances are described. In acute sera from all chickenpox patients and in the majority of acute shingles sera, antibodies reactive with glycoproteins could not be detected. In chickenpox convalescence, antibodies reactive with glycoproteins of mol. wt. 170K, 105K, 53K and 45K were identified, whilst during zoster convalescence antibodies to all six were prominent. Antibodies to the disulphide-linked glycoprotein persisted for many years following both the primary disease and its reactivation. Disseminated zoster was associated with significantly low levels of antibodies to these surface glycoproteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-8-1785
1985-08-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/8/JV0660081785.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-8-1785&mimeType=html&fmt=ahah

References

  1. Asano Y., Takahashi M. 1980; Studies on the polypeptides of varicella-zoster (V-Z) virus. Biken Journal 23:95–106
    [Google Scholar]
  2. Brunell P. A., Gershon A. A. 1973; Passive immunization against varicella-zoster infections and other modes of therapy. Journal of Infectious Diseases 127:415–423
    [Google Scholar]
  3. Brunell P. A., Ross A., Miller L. H., Kno B. 1969; Prevention of varicella by zoster immune globulin. New England Journal of Medicine 280:1191–1194
    [Google Scholar]
  4. Caunt A. E., Shaw D. G. 1969; Neutralization tests with varicella zoster virus. Journal of Hygiene 67:343–352
    [Google Scholar]
  5. Feldman S., Hughes W. T., Daniel C. B. 1975; Varicella in children with cancer. Pediatrics 56:388–397
    [Google Scholar]
  6. Gershon A. A., Steinberg S. P. 1981; Inactivation of varicella zoster virus in vitro: effect of leucocytes and specific antibody. Infection and Immunity 33:507–511
    [Google Scholar]
  7. Ghosh H. P. 1980; Synthesis and maturation of glycoproteins of enveloped animal viruses. Review of Infectious Diseases 2:26–39
    [Google Scholar]
  8. Goffinet D. R., Glatstein E. J., Merigan T. C. 1972; Herpes zoster-varicella infections and lymphoma. Annals of Internal Medicine 76:235–240
    [Google Scholar]
  9. Grose C. 1980; The synthesis of glycoproteins in human melanoma cells infected with varicella-zoster virus. Virology 101:1–9
    [Google Scholar]
  10. Grose C. 1983; Zoster in children with cancer: radioimmune profiles of sera before and after illness. Journal of Infectious Diseases 147:47–56
    [Google Scholar]
  11. Grose C., Friedrichs W. E. 1982; Immunoprecipitable polypeptides specified by varicella-zoster virus. Virology 118:86–95
    [Google Scholar]
  12. Grose C., Edwards D. P., Friedrichs W. E., Weigle K. A., McGuire W. L. 1983; Monoclonal antibodies against three major glycoproteins of varicella-zoster virus. Infection and Immunity 40:381–389
    [Google Scholar]
  13. Grose C., Edwards D. P., Weigle K. A., Friedrichs W. E., McGuire W. L. 1984; Varicella-zoster virus-specific gpl40: a highly immunogenic and disulphide-linked structural glycoprotein. Virology 132:138–146
    [Google Scholar]
  14. Heckels J. E. 1981; Structural comparison of Neisseria gonorrhoeae outer membrane proteins. Journal of Bacteriology 145:736–742
    [Google Scholar]
  15. Hicks J. T., Klutch M. J., Albrecht P., Frank M. M. 1976; Analysis of complement-dependent antibody mediated lysis of target cells acutely infected with measles. Journal of Immunology 117:208–215
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Larkin M., Ogilvie M. M., McGill J. I. 1983; Effect of low dose acyclovir (5 mg/kg) on virus shedding, interferon and humoral immunity in herpes zoster. Journal of Antimicrobial Chemotherapy 12:79–87
    [Google Scholar]
  18. Ogata M., Shigeta S. 1979; Appearance of immunoglobulin G Fc receptor in cultured human cells infected with varicella zoster virus. Infection and Immunity 26:770–774
    [Google Scholar]
  19. Russell W. C., Newman C., Williamson D. H. 1975; A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasma and viruses. Nature, London 253:461–462
    [Google Scholar]
  20. Shigeta S., Ogata M. 1979; Antibody dependent cellular protection against cell to cell spread of varicella-zoster virus. Abstract of the Annual Meeting of the American Society for Microbiology S (H) 51:304
    [Google Scholar]
  21. Shore S. L., Feorino P. M. 1981; Immunology of primary herpesvirus infections in humans. In The Human Herpesviruses: An Interdisciplinary Perspective pp 267–288 Edited by Nahmias A. J., Dowdle D. W., Schinazi R. F. New York: Elsevier;
    [Google Scholar]
  22. Sissons J. G. P., Oldstone M. B. A. 1980; Antibody mediated destruction of virus infected cells. Advances in Immunology 29:209–260
    [Google Scholar]
  23. Taylor-Robinson D. 1959; Chickenpox and herpes zoster. III. Tissue culture studies. British Journal of Experimental Pathology 40:521–532
    [Google Scholar]
  24. Weigle K. A., Grose C. 1983; Common expression of varicella-zoster viral glycoprotein antigens in vitro and in chickenpox and zoster vesicles. Journal of Infectious Diseases 148:630–638
    [Google Scholar]
  25. Weigle K. A., Grose C. 1984; Molecular dissection of the humoral immune response to individual varicella-zoster viral proteins during chickenpox, quiescence, reinfection and reactivation. Journal of Infectious Diseases 149:741–749
    [Google Scholar]
  26. Zweerink H. J., Neff B. J. 1981; Immune response after exposure to varicella zoster virus: characterisation of virus-specific antibodies and their corresponding antigens. Infection and Immunity 31:436–444
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-66-8-1785
Loading
/content/journal/jgv/10.1099/0022-1317-66-8-1785
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error