1887

Abstract

SUMMARY

Direct biochemical evidence for the existence of mutations in five of the RNA segments of the A/Leningrad/134/57 cold-adapted 47th passage mutant as compared with its wild-type progenitor has been obtained using two techniques. T oligonucleotide mapping of total viral RNA as well as of individual RNA segments revealed changes in RNAs 4, 5 and 6. Analysis of S nuclease-treated RNA–RNA hybrids on polyacrylamide gels revealed changes in at least one of the polymerase genes as well as in RNAs 4, 5, 6 and 7. These findings provide a direct demonstration for the existence of multiple mutations in the cold-adapted mutant vaccine strain.

Keyword(s): influenza A virus , mutations and vaccine
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1985-08-01
2022-01-19
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References

  1. Alexandrova G. I., Garmashova L. M., Gollubev D. B., Poliak L. I., Medvedeva T. E., Polezhaev F. I., Polyakov Y. M., Rumovskiy V. I., Smorodintsev A. A. 1979; Experience in selection of safe thermosensitive recombinants of influenza virus. Voprosi Virusologii 4:342–346
    [Google Scholar]
  2. Alexandrova G. I., Polezhaev F. I., Budilovsky G. N., Garmashova L. M., Topuria N. A., Egorov A. Y., Romejko-Gurko Y. R., Koval T. A., Lisovskaya K. V., Klimov A. I., Ghendon Y. Z. 1984; Recombinant cold-adapted attenuated influenza A vaccines for use in children: reactogenicity and antigenic activity of cold-adapted recombinants and analysis of isolates from the vaccines. Infection and Immunity 44:734–739
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1976; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brand C., Palese P. 1980; Sequential passage of influenza virus in embryonated eggs or tissue culture: emergence of mutants. Virology 107:424–433
    [Google Scholar]
  5. Clements M. L., Betts R. F., Murphy B. R. 1984; Advantage of live attenuated cold adapted influenza A virus over inactivated vaccine for A/Wasbington/80(H3N2) wild-type virus infection. Lancet 1:705–708
    [Google Scholar]
  6. Cox N. J., Kendal A. P. 1984; Genetic stability of A/Ann Arbor/6/60 cold-mutant (temperature-sensitive) live influenza virus genes: analysis by oligonucleotide mapping of recombinant vaccines before and after replication in volunteers. Journal of Infectious Diseases 149:194–200
    [Google Scholar]
  7. Cox N. J., Konnecke I., Kendal A. P., Maassab H. F. 1981; Genetic and biochemical analysis of the A/Ann Arbor/6/60 cold-adapted mutant. In ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. XXII, Genetic Variation Among Influenza Viruses pp 639–652 Edited by Nayak D. P., Fox C. F. New York: Academic Press;
    [Google Scholar]
  8. Davenport F. M., Hennessy A. V., Maassab H. F., Minuse E., Clark L. C., Abrams G. D., Mitchell J. R. 1977; Pilot studies on recombinant cold-adapted live type A and B influenza virus vaccines. Journal of Infectious Diseases 136:17–25
    [Google Scholar]
  9. Ghendon Y., Klimov A., Blagoveshenskaya O., Genkina D. 1979; Investigation of recombinants of human influenza and fowl plague viruses. Journal of General Virology 43:183–191
    [Google Scholar]
  10. Ghendon Y. Z., Klimov A. L., Alexandrova G. I., Polezhaev F. I. 1981; Analysis of genome composition and reactogenicity of recombinants of cold-adapted and virulent virus strains. Journal of General Virology 53:215–224
    [Google Scholar]
  11. Ghendon Y. Z., Polezhaev F. I., Lisovskaya K. V., Medvedeva T. E., Alexandrova G. I., Klimov A. I. 1984; Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants. Infection and Immunity 44:730–733
    [Google Scholar]
  12. Hay A. J., Bellamy A. R., Abraham G., Skehel J. J., Brand C. M., Webster R. G. 1977b; Procedures for characterizations of the genetic material of candidate vaccine strains. Developments in Biological Standardization 39:15–24
    [Google Scholar]
  13. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. 1977b; Transcription of the influenza virus genome. Virology 83:337–345
    [Google Scholar]
  14. Holland J. J., Spindler K., Horodyski F., Grabau E., Nichol S., Vandepol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  15. Hrabar A., Vodopija I., Andre F. E., Mitchell J. R., Maassab H. F., Hennessy A. V., Davenport F. M. 1977; A placebo-controlled, dose-response study of the reactogenicity and immunogenicity of a cold-adapted recombinant A/Victoria/3/75 (H3N2) live influenza virus candidate vaccine in healthy volunteers. Developments in Biological Standardization 39:53–60
    [Google Scholar]
  16. Kendal A. P., Maassab H. F., Alexandrova G. I., Ghendon Y. Z. 1981; Development of cold-adapted recombinant live, attenuated influenza A vaccines in the U.S.A. and U.S.S.R. Antiviral Research 1:339–365
    [Google Scholar]
  17. Lisovskaya K. V., Garmashova L. M., Medvedeva T. E. 1981; Analysis of ts mutations of cold-adapted influenza A/Leningrad/134/57 virus variants. Acta virologica 25:415–417
    [Google Scholar]
  18. Monath T. P., Kinney R. M., Schlesinger J. J., Brandriss M. W., Bres P. 1983; Ontogeny of yellow fever 17D vaccine: RNA oligonucleotide fingerprint and monoclonal antibody analyses of vaccines produced worldwide. Journal of General Virology 64:627–637
    [Google Scholar]
  19. Murphy B. R., Holley H. P. Jr, Berquist E. J., Levine M. M., Spring S. B., Maassab H. F., Kendal A. P., Chanock R. M. 1979; Cold-adapted variants of influenza A virus: evaluation in adult seronegative volunteers of A/Scotland/840/74 and A/Victoria/3/75 cold-adapted recombinants derived from the cold-adapted A/Ann Arbor/6/60 strains. Infection and Immunity 23:253–259
    [Google Scholar]
  20. Murphy B. R., Rennels M. B., Douglas R. G. Jr, Betts R. F., Couch R. B., Cate T. R. Jr, Chanock R. M., Kendal A. P., Maassab H. F., Suwanagool S., Sotman S. B., Cisneros L. A., Anthony W. C., Malin D. R., Leviné M. M. 1980; Evaluation of influenza A/Hong Kong/123/77 ts-IA2 and cold-adapted recombinant viruses in seronegative adult volunteers. Infection and Immunity 29:348–355
    [Google Scholar]
  21. Murphy B. R., Chanock R. M., Clements M. L., Anthony W. C., Sear A. J., Cisneros L. A., Rennels M. B., Miller E. H., Black R. E., Levine M. M., Betts R. F., Douglas R. G. Jr, Maassab H. F., Cox N. J., Kendal A. P. 1981; Evaluation of A/Alaska/6/77 (H3N2) cold-adapted recombinant viruses from A/Ann Arbor/6/60 cold-adapted donor virus in adult seronegative volunteers. Injection and Immunity 32:693–697
    [Google Scholar]
  22. Murphy B. R., Nelson D. L., Wright P. F. 1982; Secretory and systemic immunological response in children infected with live attenuated influenza A virus vaccine. Infection and Immunity 36:1102–1108
    [Google Scholar]
  23. Nottay B. K., Kew O. M., Hatch M. H., Heyward J. T., Obijeski J. F. 1980; Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans. Virology 107:405–423
    [Google Scholar]
  24. Polezhaev F. T., Garmashova L. M., Polyakov Y. M., Golubev D. B., Alexandrova G. I. 1978; Conditions for obtaining temperature-sensitive influenza virus recombinants. Acta virologica 22:263–269
    [Google Scholar]
  25. Thierry F., Spring S. B. 1981; Localization of the ts mutations of influenza A virus using complementation analysis and gel analysis of the RNA segments of recombinants. Virology 115:137–148
    [Google Scholar]
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