1887

Abstract

SUMMARY

Peripheral blood mononuclear cells (PBMC) derived from healthy individuals were stimulated with u.v.-inactivated Newcastle disease virus and the cell supernatants were assayed for both antiviral activity and alpha interferon (IFN-α) immunoreactivity. IFN-α concentrations determined by two immunoradiometric assays (IRMAs) based on monoclonal antibodies that recognize different IFN-α subtypes correlated well together ( = 0.96) and with interferon concentrations determined by the two bioassays ( = 0.82 to 0.89), but the agreement between the results of the two bioassays was not as close ( = 0.79). As judged by the agreement between determinations on duplicate inductions of the same PBMC, the IRMAs were considerably more precise than the bioassays. Despite the use of a common IFN standard there were marked differences in the absolute titres of IFN determined by the IRMAs and bioassays, highlighting the difficultires in standardizing assays for IFN-α. The IRMA results suggest that there are no major differences in the spectrum of IFN-α subtypes produced by healthy individuals under conditions of viral stimulation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-7-1621
1985-07-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/7/JV0660071621.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-7-1621&mimeType=html&fmt=ahah

References

  1. Allen G., Fantes K. H. 1980; A family of structural genes for human lymphoblastoid (leukocyte-type) interferon. Nature, London 287:408–411
    [Google Scholar]
  2. Georgiades J. A., Gumulka J., Cain B., Haywood M., Marks T., Wong D. S. 1983; Seasonal variations in human gamma production by cells induced with SEB and PHA. In The Biology of the Interferon System pp 401–406 Edited by DeMaeyer E., Schellekens H. Amsterdam, New York & London: Elsevier;
    [Google Scholar]
  3. Goeddel D. V., Leung D. W., Dull T. J., Gross M., Lawn R. M., McCandliss R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. 1981; The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature, London 290:20–26
    [Google Scholar]
  4. Gresser I., Bandu M. T., Brouty-Boye D., Tovey M. 1974; Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature, London 251:543–545
    [Google Scholar]
  5. Mogensen K., Daubas P., Gresser I., Sereni D., Varet B. 1981; Patient with circulating antibodies to α-interferon. Lancet ii:1227–1228
    [Google Scholar]
  6. Secher D. S., Burke D. C. 1980; A monoclonal antibody for large-scale purification of human leukocyte interferon. Nature, London 285:446–450
    [Google Scholar]
  7. Trown P. W., Kramer M. J., Dennin R. A., Connell E. V., Palleroni A. V. 1983; Antibodies to human leukocyte interferons in cancer patients. Lancet i:82–84
    [Google Scholar]
  8. Vallbracht A., Treuner J., Flehmig B., Joester K., Niethammer D. 1981; Interferon neutralizing antibodies in a patient treated with human fibroblast interferon. Nature, London 289:496–497
    [Google Scholar]
  9. Weck P. K., Apperson S., May L., Stebbing N. 1981; Comparison of the antiviral activities of various cloned human interferon-a subtypes in mammalian cell cultures. Journal of General Virology 57:233–237
    [Google Scholar]
  10. Wright J. F., Hunter W. M. 1983; The sucrose layering separation: a non-centrifugation system. In Immunoassay for Clinical Chemistry pp 170–177 Edited by Hunter W. M., Corrie J. E. T. Edinburgh: Churchill Livingstone;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-7-1621
Loading
/content/journal/jgv/10.1099/0022-1317-66-7-1621
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error